Links between plant and rhizoplane bacterial communities in grassland soils characterized using molecular techniques.

N. Nunan, T. J. Daniell, B. K. Singh, Artemis Loukia Papert, J. W. McNicol, James Ivor Prosser

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most closely with plants through utilization of root exudates. The aim of this study was to test the hypothesis that plant species was a major driver for bacterial rhizoplane community composition on individual plant roots. DNA extracted from individual roots was used to determine plant identity, by analysis of the plastid tRNA leucine (trnL) UAA gene intron, and plant-related bacterial communities. Bacterial communities were characterized by analysis of PCR-amplified 16S rRNA genes using two fingerprinting methods: terminal restriction fragment length polymorphisms (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Links between plant and bacterial rhizoplane communities could not be detected by visual examination of T-RFLP patterns or DGGE banding profiles. Statistical analysis of fingerprint patterns did not reveal a relationship between bacterial community composition and plant species but did demonstrate an influence of plant community composition. The data also indicated that topography and other, uncharacterized, environmental factors are important in driving bacterial community composition in grassland soils. T-RFLP had greater potential resolving power than DGGE, but findings from the two methods were not significantly different.

Original languageEnglish
Pages (from-to)6784-6792
Number of pages8
JournalApplied and Environmental Microbiology
Volume71
DOIs
Publication statusPublished - 2005

Keywords

  • GRADIENT GEL-ELECTROPHORESIS
  • POLYMERASE-CHAIN-REACTION
  • 16S RIBOSOMAL-RNA
  • MICROBIAL COMMUNITIES
  • UPLAND GRASSLAND
  • LOLIUM-PERENNE
  • SPATIAL-DISTRIBUTION
  • TRIFOLIUM-REPENS
  • BULK SOIL
  • RHIZOSPHERE

Cite this

Links between plant and rhizoplane bacterial communities in grassland soils characterized using molecular techniques. / Nunan, N.; Daniell, T. J.; Singh, B. K.; Papert, Artemis Loukia; McNicol, J. W.; Prosser, James Ivor.

In: Applied and Environmental Microbiology, Vol. 71, 2005, p. 6784-6792.

Research output: Contribution to journalArticle

Nunan, N. ; Daniell, T. J. ; Singh, B. K. ; Papert, Artemis Loukia ; McNicol, J. W. ; Prosser, James Ivor. / Links between plant and rhizoplane bacterial communities in grassland soils characterized using molecular techniques. In: Applied and Environmental Microbiology. 2005 ; Vol. 71. pp. 6784-6792.
@article{27ff211b7a174030b91e351a008047c2,
title = "Links between plant and rhizoplane bacterial communities in grassland soils characterized using molecular techniques.",
abstract = "Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed {"}bulk{"} rhizosphere soil, rather than rhizoplane communities, which interact most closely with plants through utilization of root exudates. The aim of this study was to test the hypothesis that plant species was a major driver for bacterial rhizoplane community composition on individual plant roots. DNA extracted from individual roots was used to determine plant identity, by analysis of the plastid tRNA leucine (trnL) UAA gene intron, and plant-related bacterial communities. Bacterial communities were characterized by analysis of PCR-amplified 16S rRNA genes using two fingerprinting methods: terminal restriction fragment length polymorphisms (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Links between plant and bacterial rhizoplane communities could not be detected by visual examination of T-RFLP patterns or DGGE banding profiles. Statistical analysis of fingerprint patterns did not reveal a relationship between bacterial community composition and plant species but did demonstrate an influence of plant community composition. The data also indicated that topography and other, uncharacterized, environmental factors are important in driving bacterial community composition in grassland soils. T-RFLP had greater potential resolving power than DGGE, but findings from the two methods were not significantly different.",
keywords = "GRADIENT GEL-ELECTROPHORESIS, POLYMERASE-CHAIN-REACTION, 16S RIBOSOMAL-RNA, MICROBIAL COMMUNITIES, UPLAND GRASSLAND, LOLIUM-PERENNE, SPATIAL-DISTRIBUTION, TRIFOLIUM-REPENS, BULK SOIL, RHIZOSPHERE",
author = "N. Nunan and Daniell, {T. J.} and Singh, {B. K.} and Papert, {Artemis Loukia} and McNicol, {J. W.} and Prosser, {James Ivor}",
year = "2005",
doi = "10.1128/AEM.71.11.6784-6792.2005",
language = "English",
volume = "71",
pages = "6784--6792",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - Links between plant and rhizoplane bacterial communities in grassland soils characterized using molecular techniques.

AU - Nunan, N.

AU - Daniell, T. J.

AU - Singh, B. K.

AU - Papert, Artemis Loukia

AU - McNicol, J. W.

AU - Prosser, James Ivor

PY - 2005

Y1 - 2005

N2 - Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most closely with plants through utilization of root exudates. The aim of this study was to test the hypothesis that plant species was a major driver for bacterial rhizoplane community composition on individual plant roots. DNA extracted from individual roots was used to determine plant identity, by analysis of the plastid tRNA leucine (trnL) UAA gene intron, and plant-related bacterial communities. Bacterial communities were characterized by analysis of PCR-amplified 16S rRNA genes using two fingerprinting methods: terminal restriction fragment length polymorphisms (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Links between plant and bacterial rhizoplane communities could not be detected by visual examination of T-RFLP patterns or DGGE banding profiles. Statistical analysis of fingerprint patterns did not reveal a relationship between bacterial community composition and plant species but did demonstrate an influence of plant community composition. The data also indicated that topography and other, uncharacterized, environmental factors are important in driving bacterial community composition in grassland soils. T-RFLP had greater potential resolving power than DGGE, but findings from the two methods were not significantly different.

AB - Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most closely with plants through utilization of root exudates. The aim of this study was to test the hypothesis that plant species was a major driver for bacterial rhizoplane community composition on individual plant roots. DNA extracted from individual roots was used to determine plant identity, by analysis of the plastid tRNA leucine (trnL) UAA gene intron, and plant-related bacterial communities. Bacterial communities were characterized by analysis of PCR-amplified 16S rRNA genes using two fingerprinting methods: terminal restriction fragment length polymorphisms (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Links between plant and bacterial rhizoplane communities could not be detected by visual examination of T-RFLP patterns or DGGE banding profiles. Statistical analysis of fingerprint patterns did not reveal a relationship between bacterial community composition and plant species but did demonstrate an influence of plant community composition. The data also indicated that topography and other, uncharacterized, environmental factors are important in driving bacterial community composition in grassland soils. T-RFLP had greater potential resolving power than DGGE, but findings from the two methods were not significantly different.

KW - GRADIENT GEL-ELECTROPHORESIS

KW - POLYMERASE-CHAIN-REACTION

KW - 16S RIBOSOMAL-RNA

KW - MICROBIAL COMMUNITIES

KW - UPLAND GRASSLAND

KW - LOLIUM-PERENNE

KW - SPATIAL-DISTRIBUTION

KW - TRIFOLIUM-REPENS

KW - BULK SOIL

KW - RHIZOSPHERE

U2 - 10.1128/AEM.71.11.6784-6792.2005

DO - 10.1128/AEM.71.11.6784-6792.2005

M3 - Article

VL - 71

SP - 6784

EP - 6792

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

ER -