Lipid fluorination enables phase separation from fluid phospholipid bilayers

S. J. Webb, K. Greenway, M. Bayati, Laurent Alain Claude Trembleau

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To probe the effect of lipid fluorination on the formation of lipid domains in phospholipid bilayers, several new fluorinated and non-fluorinated synthetic lipids were synthesised, and the extent of phase separation of these lipids from phospholipid bilayers of different compositions was determined. At membrane concentrations as low as 1% mol/mol, both fluorinated and non-.uorinated lipids were observed to phase separate from a gel-phase ( solid ordered) phospholipid matrix, but bilayers in a liquid disordered state caused no phase separation; if the gel-phase samples were heated above the transition temperature, then phase separation was lost. We found incorporation of perfluoroalkyl groups into the lipid enhanced phase separation, to such an extent that phase separation was observed from cholesterol containing bilayers in the liquid ordered phase.

Original languageEnglish
Pages (from-to)2399-2407
Number of pages8
JournalOrganic & Biomolecular Chemistry
Volume4
DOIs
Publication statusPublished - May 2006

Keywords

  • FLUORESCENCE CORRELATION SPECTROSCOPY
  • CELL BIOLOGY
  • MEMBRANES
  • CHOLESTEROL
  • PHOSPHATIDYLCHOLINE
  • DOMAINS
  • BINDING
  • RAFTS
  • MICROSCOPY
  • MODEL

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