Abstract
| Original language | English |
|---|---|
| Article number | 000257 |
| Number of pages | 11 |
| Journal | Microbial Genomics |
| Volume | 5 |
| Issue number | 2 |
| Early online date | 18 Feb 2019 |
| DOIs | |
| Publication status | Published - Feb 2019 |
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Keywords
- phylogeny
- human
- Listeria monocytogenes
- whole-genome sequencing
- food
- genetic diversity
- Human
- Whole-genome sequencing
- Genetic diversity
- Phylogeny
- Food
- STRAINS
- ALIGNMENT
- RESISTANCE
- SURVEILLANCE
- RISK-ASSESSMENT
ASJC Scopus subject areas
- Medicine(all)
Cite this
LiSEQ – whole-genome sequencing of a cross-sectional survey of Listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe. / Painset, Anaïs; Björkman, Jonas T.; Kiil, Kristoffer; Guillier, Laurent; Mariet, Jean-François; Félix, Benjamin; Amar, Corinne; Rotariu, Ovidiu; Roussel, Sophie; Perez-Reche, Francisco; Brisse, Sylvain; Moura, Alexandra; Lecuit, Marc; Forbes, Ken; Strachan, Norval; Grant, Kathie; Møller-Nielsen, Eva; Dallman, Timothy J. (Corresponding Author).
In: Microbial Genomics, Vol. 5, No. 2, 000257, 02.2019.Research output: Contribution to journal › Article
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TY - JOUR
T1 - LiSEQ – whole-genome sequencing of a cross-sectional survey of Listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe
AU - Painset, Anaïs
AU - Björkman, Jonas T.
AU - Kiil, Kristoffer
AU - Guillier, Laurent
AU - Mariet, Jean-François
AU - Félix, Benjamin
AU - Amar, Corinne
AU - Rotariu, Ovidiu
AU - Roussel, Sophie
AU - Perez-Reche, Francisco
AU - Brisse, Sylvain
AU - Moura, Alexandra
AU - Lecuit, Marc
AU - Forbes, Ken
AU - Strachan, Norval
AU - Grant, Kathie
AU - Møller-Nielsen, Eva
AU - Dallman, Timothy J.
N1 - Funding information This work was funded by EFSA, contract number C/EFSA/BIOCONTAM/2014/01-CT 1 on “Closing gaps for performing a risk assessment on Listeria monocytogenes in ready-to-eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis’, EFSA-Q-2014-00 026. Acknowledgements A. P., T. D. and K. G. are affiliated to the National Institute for Health Research – Health Protection Research Unit (NIHR HPRU) in Gastrointestinal Infections at University of Liverpool in partnership with Public Health England, in collaboration with the University of East Anglia, the University of Oxford and the Quadram Institute. A. P., T. D. and K. G. are based at Public Health England. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, the Department of Health or Public Health England.
PY - 2019/2
Y1 - 2019/2
N2 - We present the LiSEQ ( Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.
AB - We present the LiSEQ ( Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.
KW - phylogeny
KW - human
KW - Listeria monocytogenes
KW - whole-genome sequencing
KW - food
KW - genetic diversity
KW - Human
KW - Whole-genome sequencing
KW - Genetic diversity
KW - Phylogeny
KW - Food
KW - STRAINS
KW - ALIGNMENT
KW - RESISTANCE
KW - SURVEILLANCE
KW - RISK-ASSESSMENT
UR - http://www.scopus.com/inward/record.url?scp=85062427972&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/liseq-wholegenome-sequencing-crosssectional-survey-listeria-monocytogenes-readytoeat-foods-human-cli
U2 - 10.1099/mgen.0.000257
DO - 10.1099/mgen.0.000257
M3 - Article
VL - 5
JO - Microbial Genomics
JF - Microbial Genomics
SN - 2057-5858
IS - 2
M1 - 000257
ER -