Local and systemic antibody response in mice immunised intranasally with native and detergent-extracted outer membrane vesicles from Neisseria meningitidis

T. Guthrie, Simon Yuk Chun Wong, B. Liang, L. Hyland, S. Hou, E. A. Hoiby, S. R. Andersen

Research output: Contribution to journalArticle

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Abstract

The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associated lymphoid tissue (NALT) after three or four weekly doses. The diffuse NALT (D-NALT) contained ca. 10-fold more NOW-specific AFCs than those observed in the mediastinal lymph node, spleen, and bone marrow. AFCs observed in the D-NALT were primarily immunoglobulin A positive (IgA(+)) and were maintained for at least 1 month. In contrast, the organized NALT (O-NALT) contained low numbers of AFCs, and the response was relatively short-lived. In other lymphoid tissues, AFCs producing various IgG subclasses and IgM were present with IgG2b-producing AFCs being dominant or codominant with IgA or IgG2a. In serum and in all of the tissues examined, with the exception of the NALT, NOMVs clearly induced a stronger antibody response and a broader range of antibody isotypes than DOMVs. The development of NOMV-specific AFCs in spleen and bone marrow after intranasal immunization was slow compared to intravenous immunization but, once established, the intranasally elicited responses increased steadily for at least 75 days. NOMV-pecific antibodies induced via several routes of immunization had high bactericidal activities in serum. Our results indicated that intranasally administered OMVs induced strong local and systemic antibody responses in mice that were relatively long-lived.

Original languageEnglish
Pages (from-to)2528-2537
Number of pages9
JournalInfection and Immunity
Volume72
DOIs
Publication statusPublished - 2004

Keywords

  • CHAIN LIPOPOLYSACCHARIDE MUTANTS
  • RESPIRATORY VIRUS-INFECTION
  • NASAL LYMPHOID-TISSUE
  • GROWTH-FACTOR-BETA
  • BACTERICIDAL ACTIVITY
  • SECRETING CELLS
  • B-CELLS
  • EPITHELIAL-CELLS
  • NATURAL IMMUNITY
  • IMMUNOGLOBULIN-A

Cite this

Local and systemic antibody response in mice immunised intranasally with native and detergent-extracted outer membrane vesicles from Neisseria meningitidis. / Guthrie, T.; Wong, Simon Yuk Chun; Liang, B.; Hyland, L.; Hou, S.; Hoiby, E. A.; Andersen, S. R.

In: Infection and Immunity, Vol. 72, 2004, p. 2528-2537.

Research output: Contribution to journalArticle

Guthrie, T. ; Wong, Simon Yuk Chun ; Liang, B. ; Hyland, L. ; Hou, S. ; Hoiby, E. A. ; Andersen, S. R. / Local and systemic antibody response in mice immunised intranasally with native and detergent-extracted outer membrane vesicles from Neisseria meningitidis. In: Infection and Immunity. 2004 ; Vol. 72. pp. 2528-2537.
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abstract = "The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associated lymphoid tissue (NALT) after three or four weekly doses. The diffuse NALT (D-NALT) contained ca. 10-fold more NOW-specific AFCs than those observed in the mediastinal lymph node, spleen, and bone marrow. AFCs observed in the D-NALT were primarily immunoglobulin A positive (IgA(+)) and were maintained for at least 1 month. In contrast, the organized NALT (O-NALT) contained low numbers of AFCs, and the response was relatively short-lived. In other lymphoid tissues, AFCs producing various IgG subclasses and IgM were present with IgG2b-producing AFCs being dominant or codominant with IgA or IgG2a. In serum and in all of the tissues examined, with the exception of the NALT, NOMVs clearly induced a stronger antibody response and a broader range of antibody isotypes than DOMVs. The development of NOMV-specific AFCs in spleen and bone marrow after intranasal immunization was slow compared to intravenous immunization but, once established, the intranasally elicited responses increased steadily for at least 75 days. NOMV-pecific antibodies induced via several routes of immunization had high bactericidal activities in serum. Our results indicated that intranasally administered OMVs induced strong local and systemic antibody responses in mice that were relatively long-lived.",
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T1 - Local and systemic antibody response in mice immunised intranasally with native and detergent-extracted outer membrane vesicles from Neisseria meningitidis

AU - Guthrie, T.

AU - Wong, Simon Yuk Chun

AU - Liang, B.

AU - Hyland, L.

AU - Hou, S.

AU - Hoiby, E. A.

AU - Andersen, S. R.

PY - 2004

Y1 - 2004

N2 - The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associated lymphoid tissue (NALT) after three or four weekly doses. The diffuse NALT (D-NALT) contained ca. 10-fold more NOW-specific AFCs than those observed in the mediastinal lymph node, spleen, and bone marrow. AFCs observed in the D-NALT were primarily immunoglobulin A positive (IgA(+)) and were maintained for at least 1 month. In contrast, the organized NALT (O-NALT) contained low numbers of AFCs, and the response was relatively short-lived. In other lymphoid tissues, AFCs producing various IgG subclasses and IgM were present with IgG2b-producing AFCs being dominant or codominant with IgA or IgG2a. In serum and in all of the tissues examined, with the exception of the NALT, NOMVs clearly induced a stronger antibody response and a broader range of antibody isotypes than DOMVs. The development of NOMV-specific AFCs in spleen and bone marrow after intranasal immunization was slow compared to intravenous immunization but, once established, the intranasally elicited responses increased steadily for at least 75 days. NOMV-pecific antibodies induced via several routes of immunization had high bactericidal activities in serum. Our results indicated that intranasally administered OMVs induced strong local and systemic antibody responses in mice that were relatively long-lived.

AB - The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associated lymphoid tissue (NALT) after three or four weekly doses. The diffuse NALT (D-NALT) contained ca. 10-fold more NOW-specific AFCs than those observed in the mediastinal lymph node, spleen, and bone marrow. AFCs observed in the D-NALT were primarily immunoglobulin A positive (IgA(+)) and were maintained for at least 1 month. In contrast, the organized NALT (O-NALT) contained low numbers of AFCs, and the response was relatively short-lived. In other lymphoid tissues, AFCs producing various IgG subclasses and IgM were present with IgG2b-producing AFCs being dominant or codominant with IgA or IgG2a. In serum and in all of the tissues examined, with the exception of the NALT, NOMVs clearly induced a stronger antibody response and a broader range of antibody isotypes than DOMVs. The development of NOMV-specific AFCs in spleen and bone marrow after intranasal immunization was slow compared to intravenous immunization but, once established, the intranasally elicited responses increased steadily for at least 75 days. NOMV-pecific antibodies induced via several routes of immunization had high bactericidal activities in serum. Our results indicated that intranasally administered OMVs induced strong local and systemic antibody responses in mice that were relatively long-lived.

KW - CHAIN LIPOPOLYSACCHARIDE MUTANTS

KW - RESPIRATORY VIRUS-INFECTION

KW - NASAL LYMPHOID-TISSUE

KW - GROWTH-FACTOR-BETA

KW - BACTERICIDAL ACTIVITY

KW - SECRETING CELLS

KW - B-CELLS

KW - EPITHELIAL-CELLS

KW - NATURAL IMMUNITY

KW - IMMUNOGLOBULIN-A

U2 - 10.1128/IAI.72.5.2528-2537.2004

DO - 10.1128/IAI.72.5.2528-2537.2004

M3 - Article

VL - 72

SP - 2528

EP - 2537

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

ER -