Localisation of cannabinoid CB1 receptor-immunoreactivity in the guinea-pig and rat myenteric plexus

Angela Alice Coutts, A. J. Irving, K. Mackie, Roger Guy Pertwee, Sharon Anavi-Goffer

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Activation of cannabinoid CB, receptors inhibits gastrointestinal motility, propulsion, and transit, whereas selective antagonism of these receptors has the opposite effects, suggesting the presence of endocannabinoid tone. Supporting evidence for presynaptic CB, receptors on myenteric neurons has been found in vitro. In this study, selective CB, receptor antibodies and neuronal markers were used to identify and characterise myenteric neurons expressing cannabinoid receptors. Whole mounts of rat and guinea pig myenteric preparations were dually labelled with antibodies against the CB, receptor and choline acetyltransferase, neurofilament proteins, calbindin, calretinin, synapsin I, microtubule-associated protein-2, calcitonin gene-related peptide, or substance P. The pattern of CB, receptor labelling and the neurochemical classification of CB, receptor-positive cells were markedly influenced by the species and fixation procedure. Virtually all choline acetyltransferase-immunoreactive myenteric neurons expressed CB, receptors in ganglia from both species. Subpopulations of neurons identified with calbindin, calretinin, and microtubule-associated protein-2 did not express CB, receptors. A few calcitonin gene-related peptide- and substance P-positive somata coexpressed CB, receptor immunoreactivity but showed little colocalisation on individual fibres. There was a close association between CB, receptor immunoreactivity and fibres labelled for synaptic protein, suggesting a role in the modulation of transmitter release. Functional responses to cannabinoids in the presence of hexamethonium suggest further that CB, receptors occur on excitatory motoneurons. In conclusion, CB, receptors are expressed on a variety of cholinergic sensory, interneuronal, and motor neurons in myenteric ganglia. (C) 2002 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)410-422
Number of pages12
JournalJournal of Comparative Neurology
Volume448
Issue number4
DOIs
Publication statusPublished - Jul 2002

Keywords

  • ileum
  • cholinergic
  • neurofilament
  • hexamethonium
  • calretinin
  • synapsin I
  • primary afferent neurons
  • enteric nervous-system
  • immunohistochemical distribution
  • neurochemical classification
  • peripheral-nerve
  • small-intestine
  • localization
  • transmission
  • inhibition

Cite this

Localisation of cannabinoid CB1 receptor-immunoreactivity in the guinea-pig and rat myenteric plexus. / Coutts, Angela Alice; Irving, A. J.; Mackie, K.; Pertwee, Roger Guy; Anavi-Goffer, Sharon.

In: Journal of Comparative Neurology, Vol. 448, No. 4, 07.2002, p. 410-422.

Research output: Contribution to journalArticle

Coutts, Angela Alice ; Irving, A. J. ; Mackie, K. ; Pertwee, Roger Guy ; Anavi-Goffer, Sharon. / Localisation of cannabinoid CB1 receptor-immunoreactivity in the guinea-pig and rat myenteric plexus. In: Journal of Comparative Neurology. 2002 ; Vol. 448, No. 4. pp. 410-422.
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T1 - Localisation of cannabinoid CB1 receptor-immunoreactivity in the guinea-pig and rat myenteric plexus

AU - Coutts, Angela Alice

AU - Irving, A. J.

AU - Mackie, K.

AU - Pertwee, Roger Guy

AU - Anavi-Goffer, Sharon

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N2 - Activation of cannabinoid CB, receptors inhibits gastrointestinal motility, propulsion, and transit, whereas selective antagonism of these receptors has the opposite effects, suggesting the presence of endocannabinoid tone. Supporting evidence for presynaptic CB, receptors on myenteric neurons has been found in vitro. In this study, selective CB, receptor antibodies and neuronal markers were used to identify and characterise myenteric neurons expressing cannabinoid receptors. Whole mounts of rat and guinea pig myenteric preparations were dually labelled with antibodies against the CB, receptor and choline acetyltransferase, neurofilament proteins, calbindin, calretinin, synapsin I, microtubule-associated protein-2, calcitonin gene-related peptide, or substance P. The pattern of CB, receptor labelling and the neurochemical classification of CB, receptor-positive cells were markedly influenced by the species and fixation procedure. Virtually all choline acetyltransferase-immunoreactive myenteric neurons expressed CB, receptors in ganglia from both species. Subpopulations of neurons identified with calbindin, calretinin, and microtubule-associated protein-2 did not express CB, receptors. A few calcitonin gene-related peptide- and substance P-positive somata coexpressed CB, receptor immunoreactivity but showed little colocalisation on individual fibres. There was a close association between CB, receptor immunoreactivity and fibres labelled for synaptic protein, suggesting a role in the modulation of transmitter release. Functional responses to cannabinoids in the presence of hexamethonium suggest further that CB, receptors occur on excitatory motoneurons. In conclusion, CB, receptors are expressed on a variety of cholinergic sensory, interneuronal, and motor neurons in myenteric ganglia. (C) 2002 Wiley-Liss, Inc.

AB - Activation of cannabinoid CB, receptors inhibits gastrointestinal motility, propulsion, and transit, whereas selective antagonism of these receptors has the opposite effects, suggesting the presence of endocannabinoid tone. Supporting evidence for presynaptic CB, receptors on myenteric neurons has been found in vitro. In this study, selective CB, receptor antibodies and neuronal markers were used to identify and characterise myenteric neurons expressing cannabinoid receptors. Whole mounts of rat and guinea pig myenteric preparations were dually labelled with antibodies against the CB, receptor and choline acetyltransferase, neurofilament proteins, calbindin, calretinin, synapsin I, microtubule-associated protein-2, calcitonin gene-related peptide, or substance P. The pattern of CB, receptor labelling and the neurochemical classification of CB, receptor-positive cells were markedly influenced by the species and fixation procedure. Virtually all choline acetyltransferase-immunoreactive myenteric neurons expressed CB, receptors in ganglia from both species. Subpopulations of neurons identified with calbindin, calretinin, and microtubule-associated protein-2 did not express CB, receptors. A few calcitonin gene-related peptide- and substance P-positive somata coexpressed CB, receptor immunoreactivity but showed little colocalisation on individual fibres. There was a close association between CB, receptor immunoreactivity and fibres labelled for synaptic protein, suggesting a role in the modulation of transmitter release. Functional responses to cannabinoids in the presence of hexamethonium suggest further that CB, receptors occur on excitatory motoneurons. In conclusion, CB, receptors are expressed on a variety of cholinergic sensory, interneuronal, and motor neurons in myenteric ganglia. (C) 2002 Wiley-Liss, Inc.

KW - ileum

KW - cholinergic

KW - neurofilament

KW - hexamethonium

KW - calretinin

KW - synapsin I

KW - primary afferent neurons

KW - enteric nervous-system

KW - immunohistochemical distribution

KW - neurochemical classification

KW - peripheral-nerve

KW - small-intestine

KW - localization

KW - transmission

KW - inhibition

U2 - 10.1002/cne.10270

DO - 10.1002/cne.10270

M3 - Article

VL - 448

SP - 410

EP - 422

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 4

ER -