Localizing the biochemical transformations of arsenate in a hyperaccumulating fern

Ingrid J. Pickering, Luke Gumaelius, Hugh H. Harris, Roger C. Prince, Gregory Hirsch, Jo Ann Banks, David E. Salt, Graham N. George

Research output: Contribution to journalArticlepeer-review

160 Citations (Scopus)

Abstract

The fern Pteris vittata accumulates unusually high levels of arsenic. Using X-ray absorption spectroscopy (XAS) and XAS imaging, we reveal the distribution of arsenic species in vivo. Arsenate is transported through the vascular tissue from the roots to the fronds ( leaves), where it is reduced to arsenite and stored at high concentrations. Arsenic-thiolate species surrounding veins may be intermediates in this reduction. In gametophytes, arsenite is compartmentalized within the cell vacuole. Arsenic is excluded from cell walls, rhizoids, and reproductive areas. This study provides important insights into arsenic hyperaccumulation, which may prove useful for phytoremediating arsenic-contaminated sites, and demonstrates the strengths of XAS imaging for distinguishing highly localized species.

Original languageEnglish
Pages (from-to)5010-5014
Number of pages5
JournalEnvironmental Science & Technology
Volume40
Issue number16
Early online date12 Jul 2006
DOIs
Publication statusPublished - 15 Aug 2006

Keywords

  • Pteris-Vittata
  • Indian mustard
  • Chinese brake
  • accumulation
  • speciation
  • reductase
  • tolerance
  • transport
  • selenium
  • systems

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