Long-term use of HU210 adversely affects spermatogenesis in rats by modulating the endocannabinoid system

S. E.M. Lewis*, R. Paro, L. Borriello, L. Simon, L. Robinson, Z. Dincer, G. Riedel, N. Battista, M. Maccarrone

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recent societal acceptance of cannabinoids as recreational and therapeutic drugs has posed a potential hazard to male reproductive health. Mammals have a highly sophisticated endogenous cannabinoid (ECS) system that regulates male (and female) reproduction and exo-cannabinoids may influence it adversely. Therefore it is imperative to determine their effects on male reproduction so that men can make informed choices as to their use. Here, an animal model was used to administer HU210, a synthetic analogue of Δ9-tetrahydrocannabinol (THC) and potent cannabinoid receptor (CB) agonist to determine its effects on reproductive organ weights, spermatogenesis, testicular histology and sperm motility. Its effects on the physiological endocannabinoid system were also investigated. Spermatogenesis was markedly impaired with reductions in total sperm count after 2weeks of exposure. Spermatogenic efficiency was depleted, and Sertoli cell number decreased as exposure time increased with seminiferous tubules showing germ cell depletion developing into atrophy in some cases. Sperm motility was also adversely affected with marked reductions from 2weeks on. HU210 also acted on the sperm's endocannabinoid system. Long-term use of exo-cannabinoids has adverse effects on both spermatogenesis and sperm function. These findings highlight the urgent need for studies evaluating the fertility potential of male recreational drug users. HU210, a selective agonist for CB1 and CB2 cannabinoid receptors impairs spermatogenesis and sperm motility and deregulates the endocannabinoid system.

Original languageEnglish
Pages (from-to)731-740
Number of pages10
JournalInternational journal of andrology
Volume35
Issue number5
Early online date21 Mar 2012
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Endocannabinoids
Cannabinoids
Spermatogenesis
Sperm Motility
Cannabinoid Receptor Agonists
Street Drugs
Reproduction
Spermatozoa
Cannabinoid Receptor CB2
Cannabinoid Receptor CB1
Seminiferous Tubules
Dronabinol
Sperm Count
Organ Size
Sertoli Cells
Reproductive Health
Drug Users
Germ Cells
Atrophy
Fertility

Keywords

  • Atrophy
  • DNA
  • Endocannabinoid
  • Sertoli cell
  • Sperm
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Urology

Cite this

Lewis, S. E. M., Paro, R., Borriello, L., Simon, L., Robinson, L., Dincer, Z., ... Maccarrone, M. (2012). Long-term use of HU210 adversely affects spermatogenesis in rats by modulating the endocannabinoid system. International journal of andrology, 35(5), 731-740. https://doi.org/10.1111/j.1365-2605.2012.01259.x

Long-term use of HU210 adversely affects spermatogenesis in rats by modulating the endocannabinoid system. / Lewis, S. E.M.; Paro, R.; Borriello, L.; Simon, L.; Robinson, L.; Dincer, Z.; Riedel, G.; Battista, N.; Maccarrone, M.

In: International journal of andrology, Vol. 35, No. 5, 10.2012, p. 731-740.

Research output: Contribution to journalArticle

Lewis, SEM, Paro, R, Borriello, L, Simon, L, Robinson, L, Dincer, Z, Riedel, G, Battista, N & Maccarrone, M 2012, 'Long-term use of HU210 adversely affects spermatogenesis in rats by modulating the endocannabinoid system', International journal of andrology, vol. 35, no. 5, pp. 731-740. https://doi.org/10.1111/j.1365-2605.2012.01259.x
Lewis, S. E.M. ; Paro, R. ; Borriello, L. ; Simon, L. ; Robinson, L. ; Dincer, Z. ; Riedel, G. ; Battista, N. ; Maccarrone, M. / Long-term use of HU210 adversely affects spermatogenesis in rats by modulating the endocannabinoid system. In: International journal of andrology. 2012 ; Vol. 35, No. 5. pp. 731-740.
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AU - Maccarrone, M.

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