LUMINOMETRIC MEASUREMENT OF POPULATION ACTIVITY OF GENETICALLY MODIFIED PSEUDOMONAS-FLUORESCENS IN THE SOIL

A MEIKLE, Kenneth Stuart Killham, James Ivor Prosser, Lesley Anne Glover

Research output: Contribution to journalArticle

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Abstract

Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalFEMS Microbiology Letters
Volume99
Issue number2-3
Publication statusPublished - 1 Dec 1992

Keywords

  • MICROBIAL ACTIVITY IN THE SOIL
  • LUMINESCENCE-BASED MICROBIAL DETECTION
  • GENETICALLY MODIFIED MICROBIAL INOCULA
  • PSEUDOMONAS-FLUORESCENS
  • BIOLUMINESCENCE
  • LUMINESCENCE

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