LUMINOMETRIC MEASUREMENT OF POPULATION ACTIVITY OF GENETICALLY MODIFIED PSEUDOMONAS-FLUORESCENS IN THE SOIL

A  MEIKLE; Kenneth Stuart Killham; James Ivor Prosser; Lesley Anne Glover

LUMINOMETRIC MEASUREMENT OF POPULATION ACTIVITY OF GENETICALLY MODIFIED PSEUDOMONAS-FLUORESCENS IN THE SOIL

A MEIKLE, Kenneth Stuart Killham, James Ivor Prosser, Lesley Anne Glover

Research output: Contribution to journal › Article › peer-review

Abstract

Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

Original language	English
Pages (from-to)	217-220
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	99
Issue number	2-3
Publication status	Published - 1 Dec 1992

Keywords

MICROBIAL ACTIVITY IN THE SOIL
LUMINESCENCE-BASED MICROBIAL DETECTION
GENETICALLY MODIFIED MICROBIAL INOCULA
PSEUDOMONAS-FLUORESCENS
BIOLUMINESCENCE
LUMINESCENCE

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title = "LUMINOMETRIC MEASUREMENT OF POPULATION ACTIVITY OF GENETICALLY MODIFIED PSEUDOMONAS-FLUORESCENS IN THE SOIL",

abstract = "Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.",

keywords = "MICROBIAL ACTIVITY IN THE SOIL, LUMINESCENCE-BASED MICROBIAL DETECTION, GENETICALLY MODIFIED MICROBIAL INOCULA, PSEUDOMONAS-FLUORESCENS, BIOLUMINESCENCE, LUMINESCENCE",

author = "A MEIKLE and Killham, {Kenneth Stuart} and Prosser, {James Ivor} and Glover, {Lesley Anne}",

year = "1992",

month = dec,

day = "1",

language = "English",

volume = "99",

pages = "217--220",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "2-3",

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TY - JOUR

T1 - LUMINOMETRIC MEASUREMENT OF POPULATION ACTIVITY OF GENETICALLY MODIFIED PSEUDOMONAS-FLUORESCENS IN THE SOIL

AU - MEIKLE, A

AU - Killham, Kenneth Stuart

AU - Prosser, James Ivor

AU - Glover, Lesley Anne

PY - 1992/12/1

Y1 - 1992/12/1

N2 - Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

AB - Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

KW - MICROBIAL ACTIVITY IN THE SOIL

KW - LUMINESCENCE-BASED MICROBIAL DETECTION

KW - GENETICALLY MODIFIED MICROBIAL INOCULA

KW - PSEUDOMONAS-FLUORESCENS

KW - BIOLUMINESCENCE

KW - LUMINESCENCE

M3 - Article

VL - 99

SP - 217

EP - 220

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2-3

ER -

LUMINOMETRIC MEASUREMENT OF POPULATION ACTIVITY OF GENETICALLY MODIFIED PSEUDOMONAS-FLUORESCENS IN THE SOIL

Abstract

Keywords

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