Lung capillary albumin leak in oxygen toxicity. A quantitative immunocytochemical study

K L Weir, E N O'Gorman, J A Ross, D J Godden, A D McKinnon, P W Johnston

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The study is based on the hypothesis that lung endothelial cell permeability increases in 100% oxygen and predates the appearance of microscopically visible interstitial edema. Rats were exposed to either 100% oxygen or air in a chamber. Endogenous albumin was used as an index of permeability and measured by electron microscopic colloidal gold linked immunocytochemistry, quantified by systematic random methods. Albumin staining was expressed as relative albumin concentration (RAC), the ratio of gold particles (x 100) per point counted (gp.10(2)/pt) relating to each component. The RAC in lung perivascular/peribronchial interstitial ground substance after 24 h of hyperoxia was five times more than that of rats exposed to air for the same interval. The median value (interquartile ranges) for the oxygen-exposed group was 92.4 (39.5, 149.6) gp. 10(2)/pt compared with 14.7 (6.6, 25.9) gp. 10(2)/pt for the air-exposed group. After 60 h of 100% oxygen, the RAC was 103.4 (65.5, 148.9) gp. 10(2)/pt (60-h air exposed RAC was 11.6 (8.7, 60.4) gp. 10(2)/pt), no different from 24-h exposures. These results suggest that there was a significant leak of albumin to the perivascular/peribronchial interstitium by 24 h of exposure to 100% oxygen, which would indicate endothelial cell permeability to albumin increases earlier than has previously been reported.
Original languageEnglish
Pages (from-to)784-9
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume150
Issue number3
Publication statusPublished - 1994

Fingerprint

Albumins
Oxygen
Lung
Air
Permeability
Endothelial Cells
Gold Colloid
Hyperoxia
Prednisolone
Gold
Edema
Immunohistochemistry
Electrons
Staining and Labeling

Keywords

  • Air
  • Albumins
  • Analysis of Variance
  • Animals
  • Capillaries
  • Capillary Permeability
  • Endothelium, Vascular
  • Immunohistochemistry
  • Lung
  • Male
  • Oxygen
  • Rats
  • Rats, Sprague-Dawley
  • Specific Pathogen-Free Organisms
  • Time Factors

Cite this

Weir, K. L., O'Gorman, E. N., Ross, J. A., Godden, D. J., McKinnon, A. D., & Johnston, P. W. (1994). Lung capillary albumin leak in oxygen toxicity. A quantitative immunocytochemical study. American Journal of Respiratory and Critical Care Medicine, 150(3), 784-9.

Lung capillary albumin leak in oxygen toxicity. A quantitative immunocytochemical study. / Weir, K L; O'Gorman, E N; Ross, J A; Godden, D J; McKinnon, A D; Johnston, P W.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 150, No. 3, 1994, p. 784-9.

Research output: Contribution to journalArticle

Weir, K L ; O'Gorman, E N ; Ross, J A ; Godden, D J ; McKinnon, A D ; Johnston, P W. / Lung capillary albumin leak in oxygen toxicity. A quantitative immunocytochemical study. In: American Journal of Respiratory and Critical Care Medicine. 1994 ; Vol. 150, No. 3. pp. 784-9.
@article{5255bf6aae974d8592c46dfa1e7e0fc4,
title = "Lung capillary albumin leak in oxygen toxicity. A quantitative immunocytochemical study",
abstract = "The study is based on the hypothesis that lung endothelial cell permeability increases in 100{\%} oxygen and predates the appearance of microscopically visible interstitial edema. Rats were exposed to either 100{\%} oxygen or air in a chamber. Endogenous albumin was used as an index of permeability and measured by electron microscopic colloidal gold linked immunocytochemistry, quantified by systematic random methods. Albumin staining was expressed as relative albumin concentration (RAC), the ratio of gold particles (x 100) per point counted (gp.10(2)/pt) relating to each component. The RAC in lung perivascular/peribronchial interstitial ground substance after 24 h of hyperoxia was five times more than that of rats exposed to air for the same interval. The median value (interquartile ranges) for the oxygen-exposed group was 92.4 (39.5, 149.6) gp. 10(2)/pt compared with 14.7 (6.6, 25.9) gp. 10(2)/pt for the air-exposed group. After 60 h of 100{\%} oxygen, the RAC was 103.4 (65.5, 148.9) gp. 10(2)/pt (60-h air exposed RAC was 11.6 (8.7, 60.4) gp. 10(2)/pt), no different from 24-h exposures. These results suggest that there was a significant leak of albumin to the perivascular/peribronchial interstitium by 24 h of exposure to 100{\%} oxygen, which would indicate endothelial cell permeability to albumin increases earlier than has previously been reported.",
keywords = "Air, Albumins, Analysis of Variance, Animals, Capillaries, Capillary Permeability, Endothelium, Vascular, Immunohistochemistry, Lung, Male, Oxygen, Rats, Rats, Sprague-Dawley, Specific Pathogen-Free Organisms, Time Factors",
author = "Weir, {K L} and O'Gorman, {E N} and Ross, {J A} and Godden, {D J} and McKinnon, {A D} and Johnston, {P W}",
year = "1994",
language = "English",
volume = "150",
pages = "784--9",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society - AJRCCM",
number = "3",

}

TY - JOUR

T1 - Lung capillary albumin leak in oxygen toxicity. A quantitative immunocytochemical study

AU - Weir, K L

AU - O'Gorman, E N

AU - Ross, J A

AU - Godden, D J

AU - McKinnon, A D

AU - Johnston, P W

PY - 1994

Y1 - 1994

N2 - The study is based on the hypothesis that lung endothelial cell permeability increases in 100% oxygen and predates the appearance of microscopically visible interstitial edema. Rats were exposed to either 100% oxygen or air in a chamber. Endogenous albumin was used as an index of permeability and measured by electron microscopic colloidal gold linked immunocytochemistry, quantified by systematic random methods. Albumin staining was expressed as relative albumin concentration (RAC), the ratio of gold particles (x 100) per point counted (gp.10(2)/pt) relating to each component. The RAC in lung perivascular/peribronchial interstitial ground substance after 24 h of hyperoxia was five times more than that of rats exposed to air for the same interval. The median value (interquartile ranges) for the oxygen-exposed group was 92.4 (39.5, 149.6) gp. 10(2)/pt compared with 14.7 (6.6, 25.9) gp. 10(2)/pt for the air-exposed group. After 60 h of 100% oxygen, the RAC was 103.4 (65.5, 148.9) gp. 10(2)/pt (60-h air exposed RAC was 11.6 (8.7, 60.4) gp. 10(2)/pt), no different from 24-h exposures. These results suggest that there was a significant leak of albumin to the perivascular/peribronchial interstitium by 24 h of exposure to 100% oxygen, which would indicate endothelial cell permeability to albumin increases earlier than has previously been reported.

AB - The study is based on the hypothesis that lung endothelial cell permeability increases in 100% oxygen and predates the appearance of microscopically visible interstitial edema. Rats were exposed to either 100% oxygen or air in a chamber. Endogenous albumin was used as an index of permeability and measured by electron microscopic colloidal gold linked immunocytochemistry, quantified by systematic random methods. Albumin staining was expressed as relative albumin concentration (RAC), the ratio of gold particles (x 100) per point counted (gp.10(2)/pt) relating to each component. The RAC in lung perivascular/peribronchial interstitial ground substance after 24 h of hyperoxia was five times more than that of rats exposed to air for the same interval. The median value (interquartile ranges) for the oxygen-exposed group was 92.4 (39.5, 149.6) gp. 10(2)/pt compared with 14.7 (6.6, 25.9) gp. 10(2)/pt for the air-exposed group. After 60 h of 100% oxygen, the RAC was 103.4 (65.5, 148.9) gp. 10(2)/pt (60-h air exposed RAC was 11.6 (8.7, 60.4) gp. 10(2)/pt), no different from 24-h exposures. These results suggest that there was a significant leak of albumin to the perivascular/peribronchial interstitium by 24 h of exposure to 100% oxygen, which would indicate endothelial cell permeability to albumin increases earlier than has previously been reported.

KW - Air

KW - Albumins

KW - Analysis of Variance

KW - Animals

KW - Capillaries

KW - Capillary Permeability

KW - Endothelium, Vascular

KW - Immunohistochemistry

KW - Lung

KW - Male

KW - Oxygen

KW - Rats

KW - Rats, Sprague-Dawley

KW - Specific Pathogen-Free Organisms

KW - Time Factors

M3 - Article

C2 - 8087353

VL - 150

SP - 784

EP - 789

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 3

ER -