Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil

K Killham, L J Shaw, A A Meharg, T Boucard, L A Glover, Y Beaton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laboratory-based experiments were carried out to establish feasibility of lux-marked biosensors for assessing in-situ bioremediation potential of 2,4-DCP contaminated soil. Results showed that the decreases in 2,4-DCP concentration in soil water extracts from soil inoculated with a 2,4-DCP degrader, corresponded directly to decreases in toxicity to lux-marked E. coli. Response of the biosensor was, therefore, intimately linked to mineralisation activity in inoculated soil. In addition, light output of P. putida 12708 luxCDABE correlated with 2,4-DCP concentration in spiked sterile soil during ageing and in a field contaminated soil undergoing remediation. Lux-modified bacteria may be used as rapid, sensitive and reliable reporters of 2,4-DCP bioremediation and ageing in soil systems, complementing chemical analyses.

Original languageEnglish
Title of host publicationBioremediation of Nitroaromatic and Haloaromatic Compounds
EditorsBruce C. Alleman, Andrea Leeson
Place of PublicationColumbus
PublisherBattelle Press
Pages247-252
Number of pages6
ISBN (Print)1-57477-080-2, 9781574770803
Publication statusPublished - 1999
Event5th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO, Canada
Duration: 19 Apr 199922 Apr 1999

Conference

Conference5th International In Situ and On-Site Bioremediation Symposium
CountryCanada
CitySAN DIEGO
Period19/04/9922/04/99

Cite this

Killham, K., Shaw, L. J., Meharg, A. A., Boucard, T., Glover, L. A., & Beaton, Y. (1999). Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil. In B. C. Alleman, & A. Leeson (Eds.), Bioremediation of Nitroaromatic and Haloaromatic Compounds (pp. 247-252). Battelle Press.