Ly49B is expressed on multiple subpopulations of myeloid cells

Frances Gays, Jonathan G. Aust, Delyth Marion Reid, Jane Falconer, Noriko Toyama-Sorimachi, Philip R. Taylor, Colin G. Brooks

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on similar to 1.5% of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b(+) and comprising multiple subpopulations defined by light scatter, F4/80, and GrI expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Q(high) cells being mostly B220(+)CD4(+) and/or CD8(+), Ly49B(+) cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, alpha-IFN, and gamma-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events.

Original languageEnglish
Pages (from-to)5840-5851
Number of pages12
JournalThe Journal of Immunology
Volume177
Issue number9
Publication statusPublished - 1 Nov 2006

Keywords

  • natural killer cells
  • beta-glucan receptor
  • cysteine-rich domain
  • lectin-like domains
  • NK gene complex
  • monoclonal antibody
  • in vitro
  • cutting edge
  • T-cells
  • differentiation antigens

Cite this

Gays, F., Aust, J. G., Reid, D. M., Falconer, J., Toyama-Sorimachi, N., Taylor, P. R., & Brooks, C. G. (2006). Ly49B is expressed on multiple subpopulations of myeloid cells. The Journal of Immunology, 177(9), 5840-5851.

Ly49B is expressed on multiple subpopulations of myeloid cells. / Gays, Frances; Aust, Jonathan G.; Reid, Delyth Marion; Falconer, Jane; Toyama-Sorimachi, Noriko; Taylor, Philip R.; Brooks, Colin G.

In: The Journal of Immunology, Vol. 177, No. 9, 01.11.2006, p. 5840-5851.

Research output: Contribution to journalArticle

Gays, F, Aust, JG, Reid, DM, Falconer, J, Toyama-Sorimachi, N, Taylor, PR & Brooks, CG 2006, 'Ly49B is expressed on multiple subpopulations of myeloid cells', The Journal of Immunology, vol. 177, no. 9, pp. 5840-5851.
Gays F, Aust JG, Reid DM, Falconer J, Toyama-Sorimachi N, Taylor PR et al. Ly49B is expressed on multiple subpopulations of myeloid cells. The Journal of Immunology. 2006 Nov 1;177(9):5840-5851.
Gays, Frances ; Aust, Jonathan G. ; Reid, Delyth Marion ; Falconer, Jane ; Toyama-Sorimachi, Noriko ; Taylor, Philip R. ; Brooks, Colin G. / Ly49B is expressed on multiple subpopulations of myeloid cells. In: The Journal of Immunology. 2006 ; Vol. 177, No. 9. pp. 5840-5851.
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abstract = "Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on similar to 1.5{\%} of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b(+) and comprising multiple subpopulations defined by light scatter, F4/80, and GrI expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Q(high) cells being mostly B220(+)CD4(+) and/or CD8(+), Ly49B(+) cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, alpha-IFN, and gamma-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events.",
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T1 - Ly49B is expressed on multiple subpopulations of myeloid cells

AU - Gays, Frances

AU - Aust, Jonathan G.

AU - Reid, Delyth Marion

AU - Falconer, Jane

AU - Toyama-Sorimachi, Noriko

AU - Taylor, Philip R.

AU - Brooks, Colin G.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on similar to 1.5% of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b(+) and comprising multiple subpopulations defined by light scatter, F4/80, and GrI expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Q(high) cells being mostly B220(+)CD4(+) and/or CD8(+), Ly49B(+) cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, alpha-IFN, and gamma-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events.

AB - Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on similar to 1.5% of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b(+) and comprising multiple subpopulations defined by light scatter, F4/80, and GrI expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Q(high) cells being mostly B220(+)CD4(+) and/or CD8(+), Ly49B(+) cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, alpha-IFN, and gamma-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events.

KW - natural killer cells

KW - beta-glucan receptor

KW - cysteine-rich domain

KW - lectin-like domains

KW - NK gene complex

KW - monoclonal antibody

KW - in vitro

KW - cutting edge

KW - T-cells

KW - differentiation antigens

M3 - Article

VL - 177

SP - 5840

EP - 5851

JO - The Journal of Immunology

JF - The Journal of Immunology

SN - 0022-1767

IS - 9

ER -