Lycopene inhibits lymphocyte proliferation through mechanisms dependent on early cell activation

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8 Citations (Scopus)

Abstract

Scope
Epidemiological evidence suggests that lycopene is potentially cardio-protective. Recruitment and activation of T cells in the arterial wall is a critical process during atherogenesis, but the effects of lycopene on T-cell response remain to be elucidated. We aimed to determine whether lycopene could modulate T-cell function and activity.
Methods and results
Peripheral blood mononuclear cells from 16 healthy adults were cultured in the presence of lycopene-enriched liposomes (0–2.9 µg lycopene/mL) with or without mitogens. Cell cycle as well as the expression of CD69 (marker of early cell activation), CD25 (IL-2 receptor), and CD11a (late activation marker) were measured in T cells, T-helper cells, and T-cytotoxic cells by flow cytometry. IL-2 secretion and cell proliferation were determined by ELISA and [3H]-thymidine incorporation, respectively. Lycopene significantly inhibited lymphocyte proliferation (up to 40%) in activated cells. Lycopene also significantly inhibited CD69 expression (by up to 12%) as well as IL-2 secretion (by up to 29%). However, CD25 and CD11a expression as well as the cell-cycle profile were unaffected by lycopene.
Conclusion
Lycopene influences lymphocyte proliferation through its effects on processes involved in early cellular
Original languageEnglish
Pages (from-to)1034-1042
Number of pages9
JournalMolecular Nutrition & Food Research
Volume56
Issue number7
Early online date4 Jul 2012
DOIs
Publication statusPublished - Jul 2012

Fingerprint

lycopene
lymphocyte proliferation
Lymphocytes
T-lymphocytes
interleukin-2
cells
T-Lymphocytes
Helper-Inducer T-Lymphocytes
Interleukin-2
cell cycle
Cell Cycle
secretion
atherogenesis
Interleukin-2 Receptors
thymidine
Mitogens
Liposomes
Thymidine
flow cytometry
Blood Cells

Keywords

  • immune function
  • lycopene
  • lymphocytes
  • proliferation

Cite this

@article{033b9b40dfd34c35aa7dc654bde25212,
title = "Lycopene inhibits lymphocyte proliferation through mechanisms dependent on early cell activation",
abstract = "Scope Epidemiological evidence suggests that lycopene is potentially cardio-protective. Recruitment and activation of T cells in the arterial wall is a critical process during atherogenesis, but the effects of lycopene on T-cell response remain to be elucidated. We aimed to determine whether lycopene could modulate T-cell function and activity. Methods and results Peripheral blood mononuclear cells from 16 healthy adults were cultured in the presence of lycopene-enriched liposomes (0–2.9 µg lycopene/mL) with or without mitogens. Cell cycle as well as the expression of CD69 (marker of early cell activation), CD25 (IL-2 receptor), and CD11a (late activation marker) were measured in T cells, T-helper cells, and T-cytotoxic cells by flow cytometry. IL-2 secretion and cell proliferation were determined by ELISA and [3H]-thymidine incorporation, respectively. Lycopene significantly inhibited lymphocyte proliferation (up to 40{\%}) in activated cells. Lycopene also significantly inhibited CD69 expression (by up to 12{\%}) as well as IL-2 secretion (by up to 29{\%}). However, CD25 and CD11a expression as well as the cell-cycle profile were unaffected by lycopene. Conclusion Lycopene influences lymphocyte proliferation through its effects on processes involved in early cellular",
keywords = "immune function, lycopene, lymphocytes, proliferation",
author = "Mills, {Lynsey Margaret} and Wilson, {Heather M} and Frank Thies",
year = "2012",
month = "7",
doi = "10.1002/mnfr.201200047",
language = "English",
volume = "56",
pages = "1034--1042",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "7",

}

TY - JOUR

T1 - Lycopene inhibits lymphocyte proliferation through mechanisms dependent on early cell activation

AU - Mills, Lynsey Margaret

AU - Wilson, Heather M

AU - Thies, Frank

PY - 2012/7

Y1 - 2012/7

N2 - Scope Epidemiological evidence suggests that lycopene is potentially cardio-protective. Recruitment and activation of T cells in the arterial wall is a critical process during atherogenesis, but the effects of lycopene on T-cell response remain to be elucidated. We aimed to determine whether lycopene could modulate T-cell function and activity. Methods and results Peripheral blood mononuclear cells from 16 healthy adults were cultured in the presence of lycopene-enriched liposomes (0–2.9 µg lycopene/mL) with or without mitogens. Cell cycle as well as the expression of CD69 (marker of early cell activation), CD25 (IL-2 receptor), and CD11a (late activation marker) were measured in T cells, T-helper cells, and T-cytotoxic cells by flow cytometry. IL-2 secretion and cell proliferation were determined by ELISA and [3H]-thymidine incorporation, respectively. Lycopene significantly inhibited lymphocyte proliferation (up to 40%) in activated cells. Lycopene also significantly inhibited CD69 expression (by up to 12%) as well as IL-2 secretion (by up to 29%). However, CD25 and CD11a expression as well as the cell-cycle profile were unaffected by lycopene. Conclusion Lycopene influences lymphocyte proliferation through its effects on processes involved in early cellular

AB - Scope Epidemiological evidence suggests that lycopene is potentially cardio-protective. Recruitment and activation of T cells in the arterial wall is a critical process during atherogenesis, but the effects of lycopene on T-cell response remain to be elucidated. We aimed to determine whether lycopene could modulate T-cell function and activity. Methods and results Peripheral blood mononuclear cells from 16 healthy adults were cultured in the presence of lycopene-enriched liposomes (0–2.9 µg lycopene/mL) with or without mitogens. Cell cycle as well as the expression of CD69 (marker of early cell activation), CD25 (IL-2 receptor), and CD11a (late activation marker) were measured in T cells, T-helper cells, and T-cytotoxic cells by flow cytometry. IL-2 secretion and cell proliferation were determined by ELISA and [3H]-thymidine incorporation, respectively. Lycopene significantly inhibited lymphocyte proliferation (up to 40%) in activated cells. Lycopene also significantly inhibited CD69 expression (by up to 12%) as well as IL-2 secretion (by up to 29%). However, CD25 and CD11a expression as well as the cell-cycle profile were unaffected by lycopene. Conclusion Lycopene influences lymphocyte proliferation through its effects on processes involved in early cellular

KW - immune function

KW - lycopene

KW - lymphocytes

KW - proliferation

U2 - 10.1002/mnfr.201200047

DO - 10.1002/mnfr.201200047

M3 - Article

VL - 56

SP - 1034

EP - 1042

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 7

ER -