Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 142-149 |
| Number of pages | 8 |
| Journal | Fish & Shellfish Immunology |
| Volume | 50 |
| Early online date | 27 Jan 2016 |
| DOIs | |
| Publication status | Published - Mar 2016 |
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Keywords
- mud crab Scylla paramamosain
- Macrophage migration inhibitory factor (MIF)
- D-dopachrome tautomerase (DDT)
- cloning
- gene organisation
- expression analysis
Cite this
Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain. / Huang, Wen-Shu; Duan, Li-Peng; Huang, Bei; Wang, Ke-Jian; Zhang, Cai-Liang; Jia, Qin-qin; Nie, Pin; Wang, Tiehui.
In: Fish & Shellfish Immunology, Vol. 50, 03.2016, p. 142-149.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Macrophage migration inhibitory factor (MIF) family in arthropods
T2 - Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain
AU - Huang, Wen-Shu
AU - Duan, Li-Peng
AU - Huang, Bei
AU - Wang, Ke-Jian
AU - Zhang, Cai-Liang
AU - Jia, Qin-qin
AU - Nie, Pin
AU - Wang, Tiehui
N1 - Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No. 2012J06008 and 201311180002) and the projects-sponsored by SRF. TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.
PY - 2016/3
Y1 - 2016/3
N2 - The macrophage migration inhibitory factor (MIF) family, consisting of MIF and D-dopachrome tautomerase (DDT) in vertebrates, is evolutionarily ancient and has been found across Kingdoms including vertebrates, invertebrates, plants and bacteria. The mammalian MIF family are chemokines at the top of the inflammatory cascade in combating infections. They also possess enzymatic activities, e.g. DDT catalysis results in the production of 5,6-dihydroxyindole (DHI), a precursor of eumelanin. MIF-like genes are widely distributed, but DDT-like genes have only been described in vertebrates and a nematode. In this report, we cloned a DDT-like gene, for the first time in arthropods, and a second MIF in mud crab. The mud crab MIF family have a three exon/two intron structure as seen in vertebrates. The identification of a DDT-like gene in mud crab and other arthropods suggests that the separation of MIF and DDT preceded the divergence of protostomes and deuterostomes. The MIF family is differentially expressed in tissues of adults and during embryonic development and early life. The high level expression of the MIF family in immune tissues, such as intestine and hepatopancreas, suggests an important role in mud crab innate immunity. Mud crab DDT is highly expressed in early embryos, in megalops and crablets and this coincides with the requirement for melanisation in egg chorion tanning and cuticular hardening in arthropods, suggesting a potential novel role of DDT in melanogenesis via its tautomerase activity to produce DHI in mud crab. The clarification of the presence of both MIF and DDT in this report paves the way for further investigation of their functional roles in immunity and in melanogenesis in mud crab and other arthropods.
AB - The macrophage migration inhibitory factor (MIF) family, consisting of MIF and D-dopachrome tautomerase (DDT) in vertebrates, is evolutionarily ancient and has been found across Kingdoms including vertebrates, invertebrates, plants and bacteria. The mammalian MIF family are chemokines at the top of the inflammatory cascade in combating infections. They also possess enzymatic activities, e.g. DDT catalysis results in the production of 5,6-dihydroxyindole (DHI), a precursor of eumelanin. MIF-like genes are widely distributed, but DDT-like genes have only been described in vertebrates and a nematode. In this report, we cloned a DDT-like gene, for the first time in arthropods, and a second MIF in mud crab. The mud crab MIF family have a three exon/two intron structure as seen in vertebrates. The identification of a DDT-like gene in mud crab and other arthropods suggests that the separation of MIF and DDT preceded the divergence of protostomes and deuterostomes. The MIF family is differentially expressed in tissues of adults and during embryonic development and early life. The high level expression of the MIF family in immune tissues, such as intestine and hepatopancreas, suggests an important role in mud crab innate immunity. Mud crab DDT is highly expressed in early embryos, in megalops and crablets and this coincides with the requirement for melanisation in egg chorion tanning and cuticular hardening in arthropods, suggesting a potential novel role of DDT in melanogenesis via its tautomerase activity to produce DHI in mud crab. The clarification of the presence of both MIF and DDT in this report paves the way for further investigation of their functional roles in immunity and in melanogenesis in mud crab and other arthropods.
KW - mud crab Scylla paramamosain
KW - Macrophage migration inhibitory factor (MIF)
KW - D-dopachrome tautomerase (DDT)
KW - cloning
KW - gene organisation
KW - expression analysis
U2 - 10.1016/j.fsi.2016.01.030
DO - 10.1016/j.fsi.2016.01.030
M3 - Article
VL - 50
SP - 142
EP - 149
JO - Fish & Shellfish Immunology
JF - Fish & Shellfish Immunology
SN - 1050-4648
ER -