Macrophage Migration Is Impaired within Candida albicans Biofilms

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Abstract

Candida albicans is an opportunistic fungal pathogen that infects immunocompromised patients. Infection control requires phagocytosis by innate immune cells, including macrophages. Migration towards, and subsequent recognition of, C. albicans fungal cell wall components by macrophages is critical for phagocytosis. Using live-cell imaging of phagocytosis, the macrophage cell line J774.1 showed enhanced movement in response to C. albicans cell wall mutants, particularly during the first 30 min, irrespective of the infection ratio. However, phagocyte migration was reduced up to 2-fold within a C. albicans biofilm compared to planktonic fungal cells. Biofilms formed from C. albicans glycosylation mutant cells also inhibited macrophage migration to a similar extent as wildtype Candida biofilms, suggesting that the physical structure of the biofilm, rather than polysaccharide matrix composition, may hamper phagocyte migration. These data illustrate differential macrophage migratory capacities, dependent upon the form of C. albicans encountered. Impaired migration of macrophages within a C. albicans biofilm may contribute to the recalcitrant nature of clinical infections in which biofilm formation occurs.
Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalJournal of Fungi
Volume3
Issue number3
Early online date22 Jun 2017
DOIs
Publication statusPublished - 22 Jun 2017

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Biofilms
Candida albicans
Macrophages
Phagocytes
Phagocytosis
Cell Wall
Cytophagocytosis
Immunocompromised Host
Cellular Structures
Infection Control
Infection
Glycosylation
Candida
Polysaccharides
Cell Line

Keywords

  • Candida
  • biofilm
  • macrophage
  • phagocytosis
  • migration
  • cell wall

Cite this

Macrophage Migration Is Impaired within Candida albicans Biofilms. / Alonso, Maria F.; Gow, Neil A. R.; Erwig, Lars P.; Bain, Judith M.

In: Journal of Fungi, Vol. 3, No. 3, 22.06.2017, p. 1-12.

Research output: Contribution to journalArticle

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