Abstract
Maintenance coefficients and rates of turnover of cell protein, cell walls and total cell phosphate were determined during the growth of Escherichia coli ML308 in fed-batch culture at different temperatures. The apparent Arrhenius activation energies (Ea) calculated for maintenance coefficients at temperatures over 30°C were 212–515 kJ· mol−1, indicative of a process thermodynamically similar to denaturation. The corresponding Arrhenius values for turnover were of a different magnitude, 44–115 kJ mol−1, more typical of enzyme-catalysed reactions. Addition of 0.5 M NaCl had a minor effect on maintenance at both temperatures. Turnover of cell material and salt homeostasis were therefore concluded to be minor components of the maintenance requirement of E. coli.
Original language | English |
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Pages (from-to) | 317-320 |
Number of pages | 4 |
Journal | FEMS Microbiology Letters |
Volume | 37 |
Issue number | 3 |
DOIs | |
Publication status | Published - Dec 1986 |