Maintenance coefficients and rates of turnover of cell material in Escherichia coli ML308 at different growth temperatures

R. J. Wallace, W. H. Holms

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Abstract

Maintenance coefficients and rates of turnover of cell protein, cell walls and total cell phosphate were determined during the growth of Escherichia coli ML308 in fed-batch culture at different temperatures. The apparent Arrhenius activation energies (Ea) calculated for maintenance coefficients at temperatures over 30°C were 212–515 kJ· mol−1, indicative of a process thermodynamically similar to denaturation. The corresponding Arrhenius values for turnover were of a different magnitude, 44–115 kJ mol−1, more typical of enzyme-catalysed reactions. Addition of 0.5 M NaCl had a minor effect on maintenance at both temperatures. Turnover of cell material and salt homeostasis were therefore concluded to be minor components of the maintenance requirement of E. coli.
Original languageEnglish
Pages (from-to)317-320
Number of pages4
JournalFEMS Microbiology Letters
Volume37
Issue number3
DOIs
Publication statusPublished - Dec 1986

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Maintenance
Escherichia coli
Temperature
Growth
Batch Cell Culture Techniques
Cell Wall
Homeostasis
Salts
Phosphates
Enzymes
Proteins

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Maintenance coefficients and rates of turnover of cell material in Escherichia coli ML308 at different growth temperatures. / Wallace, R. J.; Holms, W. H.

In: FEMS Microbiology Letters, Vol. 37, No. 3, 12.1986, p. 317-320.

Research output: Contribution to journalArticle

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