Major histocompatibility complex B-LB gene variation in red grouse (Lagopus lagopus scoticus)

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16 Citations (Scopus)

Abstract

Major histocompatibility complex (MHC) variation at the class Up (B-LB) gene was surveyed both within and among populations of red grouse Lagopus lagopus scoticus from northeastern Scotland. Variation among individuals was assayed by direct DNA sequencing, with 13 unique alleles being resolved among 10 unrelated individuals. All nucleotide substitutions were non-synonymous (amino acid changing) substitutions. Variation among five populations was screened using single stranded conformational polymorphism (SSCP). Twelve B-LB alleles were resolved, with low levels of genetic structure between the populations. This is in contrast to the considerable genetic differentiation observed among the same populations determined from microsatellite DNA analysis. Both the patterns of nucleotide substitution within individuals, and the discrepancy between MHC and microsatellite derived patterns of population genetic structure highlight that balancing selection is acting to maintain variation in the grouse MHC. The significance of MHC dynamics and diversity is discussed in the context of grouse ecology and conservation.

Original languageEnglish
Pages (from-to)251-259
Number of pages8
JournalWildlife Biology
Volume9
Publication statusPublished - 2003

Keywords

  • conservation
  • grouse
  • Lagopus
  • MHC
  • microsatellite
  • selection
  • TRICHOSTRONGYLUS-TENUIS
  • MHC POLYMORPHISM
  • POPULATION
  • SELECTION
  • PARASITE
  • LOCI
  • DYNAMICS
  • BIRDS

Cite this

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title = "Major histocompatibility complex B-LB gene variation in red grouse (Lagopus lagopus scoticus)",
abstract = "Major histocompatibility complex (MHC) variation at the class Up (B-LB) gene was surveyed both within and among populations of red grouse Lagopus lagopus scoticus from northeastern Scotland. Variation among individuals was assayed by direct DNA sequencing, with 13 unique alleles being resolved among 10 unrelated individuals. All nucleotide substitutions were non-synonymous (amino acid changing) substitutions. Variation among five populations was screened using single stranded conformational polymorphism (SSCP). Twelve B-LB alleles were resolved, with low levels of genetic structure between the populations. This is in contrast to the considerable genetic differentiation observed among the same populations determined from microsatellite DNA analysis. Both the patterns of nucleotide substitution within individuals, and the discrepancy between MHC and microsatellite derived patterns of population genetic structure highlight that balancing selection is acting to maintain variation in the grouse MHC. The significance of MHC dynamics and diversity is discussed in the context of grouse ecology and conservation.",
keywords = "conservation, grouse, Lagopus, MHC, microsatellite, selection, TRICHOSTRONGYLUS-TENUIS, MHC POLYMORPHISM, POPULATION, SELECTION, PARASITE, LOCI, DYNAMICS, BIRDS",
author = "Piertney, {Stuart Brannon}",
year = "2003",
language = "English",
volume = "9",
pages = "251--259",
journal = "Wildlife Biology",
issn = "0909-6396",
publisher = "Nordic Council for Wildlife Research",

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TY - JOUR

T1 - Major histocompatibility complex B-LB gene variation in red grouse (Lagopus lagopus scoticus)

AU - Piertney, Stuart Brannon

PY - 2003

Y1 - 2003

N2 - Major histocompatibility complex (MHC) variation at the class Up (B-LB) gene was surveyed both within and among populations of red grouse Lagopus lagopus scoticus from northeastern Scotland. Variation among individuals was assayed by direct DNA sequencing, with 13 unique alleles being resolved among 10 unrelated individuals. All nucleotide substitutions were non-synonymous (amino acid changing) substitutions. Variation among five populations was screened using single stranded conformational polymorphism (SSCP). Twelve B-LB alleles were resolved, with low levels of genetic structure between the populations. This is in contrast to the considerable genetic differentiation observed among the same populations determined from microsatellite DNA analysis. Both the patterns of nucleotide substitution within individuals, and the discrepancy between MHC and microsatellite derived patterns of population genetic structure highlight that balancing selection is acting to maintain variation in the grouse MHC. The significance of MHC dynamics and diversity is discussed in the context of grouse ecology and conservation.

AB - Major histocompatibility complex (MHC) variation at the class Up (B-LB) gene was surveyed both within and among populations of red grouse Lagopus lagopus scoticus from northeastern Scotland. Variation among individuals was assayed by direct DNA sequencing, with 13 unique alleles being resolved among 10 unrelated individuals. All nucleotide substitutions were non-synonymous (amino acid changing) substitutions. Variation among five populations was screened using single stranded conformational polymorphism (SSCP). Twelve B-LB alleles were resolved, with low levels of genetic structure between the populations. This is in contrast to the considerable genetic differentiation observed among the same populations determined from microsatellite DNA analysis. Both the patterns of nucleotide substitution within individuals, and the discrepancy between MHC and microsatellite derived patterns of population genetic structure highlight that balancing selection is acting to maintain variation in the grouse MHC. The significance of MHC dynamics and diversity is discussed in the context of grouse ecology and conservation.

KW - conservation

KW - grouse

KW - Lagopus

KW - MHC

KW - microsatellite

KW - selection

KW - TRICHOSTRONGYLUS-TENUIS

KW - MHC POLYMORPHISM

KW - POPULATION

KW - SELECTION

KW - PARASITE

KW - LOCI

KW - DYNAMICS

KW - BIRDS

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M3 - Article

VL - 9

SP - 251

EP - 259

JO - Wildlife Biology

JF - Wildlife Biology

SN - 0909-6396

ER -