Major Histocompatibility Complex (MHC) Class II sequence polymorphism in long-finned pilot whale (Globicephala melas) from the North Atlantic

Silvia S. Monteiro, Jose V. Vingada, Alfredo Lopez, Graham J. Pierce, Marisa Ferreira, Andrew Brownlow, Bjarni Mikkelsen, Misty Niemeyer, Robert J. Deaville, Catarina Eira, Stuart Piertney

Research output: Contribution to journalArticle

5 Downloads (Pure)

Abstract

Determining how intra-specific genetic diversity is apportioned among natural populations is essential for detecting local adaptation and identifying populations with inherently low levels of extant diversity which may become a conservation concern. Sequence polymorphism at two adaptive loci (MHC DRA and DQB) was investigated in long-finned pilot whales (Globicephala melas) from four regions in the North Atlantic and compared with previous data from New Zealand (South Pacific). Three alleles were resolved at each locus, with trans-species allele sharing and higher levels of non-synonymous to synonymous substitution, especially in the DQB locus. Overall nucleotide diversities of 0.49 ± 0.38% and 4.60 ± 2.39% were identified for the DRA and DQB loci, respectively, which are relatively low for MHC loci in the North Atlantic, but comparable to levels previously described in New Zealand (South Pacific). There were significant differences in allele frequencies within the North Atlantic and between the North Atlantic and New Zealand. Patterns of diversity and divergence are consistent with the long-term effects of balancing selection operating on the MHC loci, potentially mediated through the effects of host-parasite coevolution. Differences in allele frequency may reflect variation in pathogen communities, coupled with the effects of differential drift and gene flow.
Original languageEnglish
Pages (from-to)595-607
Number of pages13
JournalMarine Biology Research
Volume12
Issue number6
Early online date15 Jun 2016
DOIs
Publication statusPublished - 2016

Fingerprint

major histocompatibility complex
whales
whale
allele
polymorphism
genetic polymorphism
loci
gene frequency
local adaptation
coevolution
gene flow
alleles
parasite
substitution
pathogen
divergence
long term effects
nucleotides
parasites
genetic variation

Keywords

  • adaptive markers
  • evolution
  • cetacea

Cite this

Major Histocompatibility Complex (MHC) Class II sequence polymorphism in long-finned pilot whale (Globicephala melas) from the North Atlantic. / Monteiro, Silvia S.; Vingada, Jose V.; Lopez, Alfredo; Pierce, Graham J.; Ferreira, Marisa; Brownlow, Andrew; Mikkelsen, Bjarni; Niemeyer, Misty; Deaville, Robert J.; Eira, Catarina; Piertney, Stuart.

In: Marine Biology Research, Vol. 12, No. 6, 2016, p. 595-607.

Research output: Contribution to journalArticle

Monteiro, SS, Vingada, JV, Lopez, A, Pierce, GJ, Ferreira, M, Brownlow, A, Mikkelsen, B, Niemeyer, M, Deaville, RJ, Eira, C & Piertney, S 2016, 'Major Histocompatibility Complex (MHC) Class II sequence polymorphism in long-finned pilot whale (Globicephala melas) from the North Atlantic', Marine Biology Research, vol. 12, no. 6, pp. 595-607. https://doi.org/10.1080/17451000.2016.1174266
Monteiro, Silvia S. ; Vingada, Jose V. ; Lopez, Alfredo ; Pierce, Graham J. ; Ferreira, Marisa ; Brownlow, Andrew ; Mikkelsen, Bjarni ; Niemeyer, Misty ; Deaville, Robert J. ; Eira, Catarina ; Piertney, Stuart. / Major Histocompatibility Complex (MHC) Class II sequence polymorphism in long-finned pilot whale (Globicephala melas) from the North Atlantic. In: Marine Biology Research. 2016 ; Vol. 12, No. 6. pp. 595-607.
@article{7e0aff0c71a048f6b8c6268f8ef0327b,
title = "Major Histocompatibility Complex (MHC) Class II sequence polymorphism in long-finned pilot whale (Globicephala melas) from the North Atlantic",
abstract = "Determining how intra-specific genetic diversity is apportioned among natural populations is essential for detecting local adaptation and identifying populations with inherently low levels of extant diversity which may become a conservation concern. Sequence polymorphism at two adaptive loci (MHC DRA and DQB) was investigated in long-finned pilot whales (Globicephala melas) from four regions in the North Atlantic and compared with previous data from New Zealand (South Pacific). Three alleles were resolved at each locus, with trans-species allele sharing and higher levels of non-synonymous to synonymous substitution, especially in the DQB locus. Overall nucleotide diversities of 0.49 ± 0.38{\%} and 4.60 ± 2.39{\%} were identified for the DRA and DQB loci, respectively, which are relatively low for MHC loci in the North Atlantic, but comparable to levels previously described in New Zealand (South Pacific). There were significant differences in allele frequencies within the North Atlantic and between the North Atlantic and New Zealand. Patterns of diversity and divergence are consistent with the long-term effects of balancing selection operating on the MHC loci, potentially mediated through the effects of host-parasite coevolution. Differences in allele frequency may reflect variation in pathogen communities, coupled with the effects of differential drift and gene flow.",
keywords = "adaptive markers, evolution , cetacea",
author = "Monteiro, {Silvia S.} and Vingada, {Jose V.} and Alfredo Lopez and Pierce, {Graham J.} and Marisa Ferreira and Andrew Brownlow and Bjarni Mikkelsen and Misty Niemeyer and Deaville, {Robert J.} and Catarina Eira and Stuart Piertney",
note = "Funding Silvia S. Monteiro and Marisa Ferreira were supported by a Ph.D. grant from Funda{\cc}{\~a}o para a Ci{\^e}ncia e Tecnologia (ref SFRH/BD/38735/2007 and SFRH/BD/30240/2006, respectively). Alfredo L{\'o}pez was supported by a postdoctoral grant from Funda{\cc}{\~a}o para a Ci{\^e}ncia e Tecnologia (ref SFRH/BPD/82407/2011). Catarina Eira is supported by CESAM (UID/AMB/50017), from FCT/MEC through national funds and FEDER (PT2020, Compete 2020). The work related with strandings and tissue collection in Portugal was partially supported by the SafeSea Project EEAGrants PT 0039 (supported by Iceland, Liechtenstein and Norway through the EEA Financial Mechanism), by the Project MarPro–Life09 NAT/PT/000038 (funded by the European Union–Program Life+) and by the project CetSenti FCT RECI/AAG-GLO/0470/2012; FCOMP-01-0124-FEDER-027472 (Funded by the Program COMPETE and Funda{\cc}{\~a}o para a Ci{\^e}ncia e Tecnologia).",
year = "2016",
doi = "10.1080/17451000.2016.1174266",
language = "English",
volume = "12",
pages = "595--607",
journal = "Marine Biology Research",
issn = "1745-1000",
publisher = "TAYLOR & FRANCIS AS",
number = "6",

}

TY - JOUR

T1 - Major Histocompatibility Complex (MHC) Class II sequence polymorphism in long-finned pilot whale (Globicephala melas) from the North Atlantic

AU - Monteiro, Silvia S.

AU - Vingada, Jose V.

AU - Lopez, Alfredo

AU - Pierce, Graham J.

AU - Ferreira, Marisa

AU - Brownlow, Andrew

AU - Mikkelsen, Bjarni

AU - Niemeyer, Misty

AU - Deaville, Robert J.

AU - Eira, Catarina

AU - Piertney, Stuart

N1 - Funding Silvia S. Monteiro and Marisa Ferreira were supported by a Ph.D. grant from Fundação para a Ciência e Tecnologia (ref SFRH/BD/38735/2007 and SFRH/BD/30240/2006, respectively). Alfredo López was supported by a postdoctoral grant from Fundação para a Ciência e Tecnologia (ref SFRH/BPD/82407/2011). Catarina Eira is supported by CESAM (UID/AMB/50017), from FCT/MEC through national funds and FEDER (PT2020, Compete 2020). The work related with strandings and tissue collection in Portugal was partially supported by the SafeSea Project EEAGrants PT 0039 (supported by Iceland, Liechtenstein and Norway through the EEA Financial Mechanism), by the Project MarPro–Life09 NAT/PT/000038 (funded by the European Union–Program Life+) and by the project CetSenti FCT RECI/AAG-GLO/0470/2012; FCOMP-01-0124-FEDER-027472 (Funded by the Program COMPETE and Fundação para a Ciência e Tecnologia).

PY - 2016

Y1 - 2016

N2 - Determining how intra-specific genetic diversity is apportioned among natural populations is essential for detecting local adaptation and identifying populations with inherently low levels of extant diversity which may become a conservation concern. Sequence polymorphism at two adaptive loci (MHC DRA and DQB) was investigated in long-finned pilot whales (Globicephala melas) from four regions in the North Atlantic and compared with previous data from New Zealand (South Pacific). Three alleles were resolved at each locus, with trans-species allele sharing and higher levels of non-synonymous to synonymous substitution, especially in the DQB locus. Overall nucleotide diversities of 0.49 ± 0.38% and 4.60 ± 2.39% were identified for the DRA and DQB loci, respectively, which are relatively low for MHC loci in the North Atlantic, but comparable to levels previously described in New Zealand (South Pacific). There were significant differences in allele frequencies within the North Atlantic and between the North Atlantic and New Zealand. Patterns of diversity and divergence are consistent with the long-term effects of balancing selection operating on the MHC loci, potentially mediated through the effects of host-parasite coevolution. Differences in allele frequency may reflect variation in pathogen communities, coupled with the effects of differential drift and gene flow.

AB - Determining how intra-specific genetic diversity is apportioned among natural populations is essential for detecting local adaptation and identifying populations with inherently low levels of extant diversity which may become a conservation concern. Sequence polymorphism at two adaptive loci (MHC DRA and DQB) was investigated in long-finned pilot whales (Globicephala melas) from four regions in the North Atlantic and compared with previous data from New Zealand (South Pacific). Three alleles were resolved at each locus, with trans-species allele sharing and higher levels of non-synonymous to synonymous substitution, especially in the DQB locus. Overall nucleotide diversities of 0.49 ± 0.38% and 4.60 ± 2.39% were identified for the DRA and DQB loci, respectively, which are relatively low for MHC loci in the North Atlantic, but comparable to levels previously described in New Zealand (South Pacific). There were significant differences in allele frequencies within the North Atlantic and between the North Atlantic and New Zealand. Patterns of diversity and divergence are consistent with the long-term effects of balancing selection operating on the MHC loci, potentially mediated through the effects of host-parasite coevolution. Differences in allele frequency may reflect variation in pathogen communities, coupled with the effects of differential drift and gene flow.

KW - adaptive markers

KW - evolution

KW - cetacea

U2 - 10.1080/17451000.2016.1174266

DO - 10.1080/17451000.2016.1174266

M3 - Article

VL - 12

SP - 595

EP - 607

JO - Marine Biology Research

JF - Marine Biology Research

SN - 1745-1000

IS - 6

ER -