Mammary gland development is delayed in mice deficient for aminopeptidase N

Andreas F Kolb, David Sorrell, Caroline Lassnig, Simon Lillico, Ailsa Carlisle, Claire Neil, Claire Robinson, Mathias Müller, C Bruce A Whitelaw

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Development of the mammary gland requires the coordinated action of proteolytic enzymes during two phases of remodelling. Firstly, new ducts and side-branches thereof need to be established during pregnancy to generate an extensive ductal tree allowing the secretion and transport of milk. A second wave of remodelling occurs during mammary involution after weaning. We have analysed the role of the cell surface protease aminopeptidase N (Anpep, APN, CD13) during these processes using Anpep deficient and Anpep over-expressing mice. We find that APN deficiency significantly delays mammary gland morphogenesis during gestation. The defect is characterised by a reduction in alveolar buds and duct branching at mid-pregnancy. Conversely over-expression of Anpep leads to accelerated ductal development. This indicates that Anpep plays a critical role in the proteolytic remodelling of mammary tissue during adult mammary development.
Original languageEnglish
Pages (from-to)425-434
Number of pages10
JournalTransgenic Research
Volume22
Issue number2
Early online date15 Sep 2012
DOIs
Publication statusPublished - Apr 2013

Fingerprint

membrane alanyl aminopeptidase
CD13 Antigens
Human Mammary Glands
mammary glands
Breast
pregnancy
Pregnancy
mice
Peptide Hydrolases
mammary involution
mammary development
Weaning
Morphogenesis
breasts
morphogenesis
branching
weaning
Milk
proteinases
buds

Keywords

  • mammary gland
  • protease
  • gene locus
  • homologous recombination
  • development

Cite this

Kolb, A. F., Sorrell, D., Lassnig, C., Lillico, S., Carlisle, A., Neil, C., ... Whitelaw, C. B. A. (2013). Mammary gland development is delayed in mice deficient for aminopeptidase N. Transgenic Research, 22(2), 425-434. https://doi.org/10.1007/s11248-012-9654-7

Mammary gland development is delayed in mice deficient for aminopeptidase N. / Kolb, Andreas F; Sorrell, David; Lassnig, Caroline; Lillico, Simon; Carlisle, Ailsa; Neil, Claire; Robinson, Claire; Müller, Mathias; Whitelaw, C Bruce A.

In: Transgenic Research, Vol. 22, No. 2, 04.2013, p. 425-434.

Research output: Contribution to journalArticle

Kolb, AF, Sorrell, D, Lassnig, C, Lillico, S, Carlisle, A, Neil, C, Robinson, C, Müller, M & Whitelaw, CBA 2013, 'Mammary gland development is delayed in mice deficient for aminopeptidase N', Transgenic Research, vol. 22, no. 2, pp. 425-434. https://doi.org/10.1007/s11248-012-9654-7
Kolb, Andreas F ; Sorrell, David ; Lassnig, Caroline ; Lillico, Simon ; Carlisle, Ailsa ; Neil, Claire ; Robinson, Claire ; Müller, Mathias ; Whitelaw, C Bruce A. / Mammary gland development is delayed in mice deficient for aminopeptidase N. In: Transgenic Research. 2013 ; Vol. 22, No. 2. pp. 425-434.
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