Mapping and sequencing rat dishevelled-1

a candidate gene for cerebral ischaemic insult in a rat model of stroke

R. P. De Lange, K. Burr, J. S. Clark, C. D. Negrin, M. J. Brosnan, David Malcolm St Clair, A. F. Dominiczak, Duncan James Shaw

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A quantitative trait locus on chromosome 5 in the rat is linked to sensitivity to brain ischemia in the stroke-prone spontaneously hypertensive rat (SHRSP). The genes encoding atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) that map to this location have been excluded as candidate genes. We examined dishevelled-1 (DVL-1) as a further candidate gene. DVL-1 had not yet been identified in the rat, but Anp, Bnp, and DVL-1 map to the homologous regions of the rat chromosome 5 quantitative trait locus in both mice and man. Furthermore, DVL-1 is involved in the Notch signalling system, which plays a role in the disorder cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, the symptoms of which include ischaemic stroke. We show with radiation hybrid mapping that rat DVL-1 indeed maps to chromosome 5, where it is positioned immediately next to microsatellite marker D5Rat49. We sequenced the complete coding sequence and a large part of the intronic genomic sequence for the SHRSP strain and its reference Wistar-Kyoto strain. The DVL-1 sequence in the two strains was identical. Our results essentially exclude the DVL-1 gene as the cause for sensitivity to cerebral ischaemic insult in this rat model of stroke.

Original languageEnglish
Pages (from-to)99-106
Number of pages7
JournalNeurogenetics
Volume3
Issue number2
DOIs
Publication statusPublished - 2001

Keywords

  • genomic structure
  • chromosomal location
  • dishevelled
  • hypertension
  • rat
  • SPONTANEOUSLY HYPERTENSIVE RATS
  • NOTCH3 MUTATIONS
  • POLARITY GENE
  • PRONE
  • SENSITIVITY
  • CADASIL
  • TRAIT

Cite this

Mapping and sequencing rat dishevelled-1 : a candidate gene for cerebral ischaemic insult in a rat model of stroke. / De Lange, R. P.; Burr, K.; Clark, J. S.; Negrin, C. D.; Brosnan, M. J.; St Clair, David Malcolm; Dominiczak, A. F.; Shaw, Duncan James.

In: Neurogenetics, Vol. 3, No. 2, 2001, p. 99-106.

Research output: Contribution to journalArticle

De Lange, R. P. ; Burr, K. ; Clark, J. S. ; Negrin, C. D. ; Brosnan, M. J. ; St Clair, David Malcolm ; Dominiczak, A. F. ; Shaw, Duncan James. / Mapping and sequencing rat dishevelled-1 : a candidate gene for cerebral ischaemic insult in a rat model of stroke. In: Neurogenetics. 2001 ; Vol. 3, No. 2. pp. 99-106.
@article{9e3c2e8bb6ef4c6eb0cb161cdd0310d4,
title = "Mapping and sequencing rat dishevelled-1: a candidate gene for cerebral ischaemic insult in a rat model of stroke",
abstract = "A quantitative trait locus on chromosome 5 in the rat is linked to sensitivity to brain ischemia in the stroke-prone spontaneously hypertensive rat (SHRSP). The genes encoding atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) that map to this location have been excluded as candidate genes. We examined dishevelled-1 (DVL-1) as a further candidate gene. DVL-1 had not yet been identified in the rat, but Anp, Bnp, and DVL-1 map to the homologous regions of the rat chromosome 5 quantitative trait locus in both mice and man. Furthermore, DVL-1 is involved in the Notch signalling system, which plays a role in the disorder cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, the symptoms of which include ischaemic stroke. We show with radiation hybrid mapping that rat DVL-1 indeed maps to chromosome 5, where it is positioned immediately next to microsatellite marker D5Rat49. We sequenced the complete coding sequence and a large part of the intronic genomic sequence for the SHRSP strain and its reference Wistar-Kyoto strain. The DVL-1 sequence in the two strains was identical. Our results essentially exclude the DVL-1 gene as the cause for sensitivity to cerebral ischaemic insult in this rat model of stroke.",
keywords = "genomic structure, chromosomal location, dishevelled, hypertension, rat, SPONTANEOUSLY HYPERTENSIVE RATS, NOTCH3 MUTATIONS, POLARITY GENE, PRONE, SENSITIVITY, CADASIL, TRAIT",
author = "{De Lange}, {R. P.} and K. Burr and Clark, {J. S.} and Negrin, {C. D.} and Brosnan, {M. J.} and {St Clair}, {David Malcolm} and Dominiczak, {A. F.} and Shaw, {Duncan James}",
year = "2001",
doi = "10.1007/s100480000099",
language = "English",
volume = "3",
pages = "99--106",
journal = "Neurogenetics",
issn = "1364-6745",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Mapping and sequencing rat dishevelled-1

T2 - a candidate gene for cerebral ischaemic insult in a rat model of stroke

AU - De Lange, R. P.

AU - Burr, K.

AU - Clark, J. S.

AU - Negrin, C. D.

AU - Brosnan, M. J.

AU - St Clair, David Malcolm

AU - Dominiczak, A. F.

AU - Shaw, Duncan James

PY - 2001

Y1 - 2001

N2 - A quantitative trait locus on chromosome 5 in the rat is linked to sensitivity to brain ischemia in the stroke-prone spontaneously hypertensive rat (SHRSP). The genes encoding atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) that map to this location have been excluded as candidate genes. We examined dishevelled-1 (DVL-1) as a further candidate gene. DVL-1 had not yet been identified in the rat, but Anp, Bnp, and DVL-1 map to the homologous regions of the rat chromosome 5 quantitative trait locus in both mice and man. Furthermore, DVL-1 is involved in the Notch signalling system, which plays a role in the disorder cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, the symptoms of which include ischaemic stroke. We show with radiation hybrid mapping that rat DVL-1 indeed maps to chromosome 5, where it is positioned immediately next to microsatellite marker D5Rat49. We sequenced the complete coding sequence and a large part of the intronic genomic sequence for the SHRSP strain and its reference Wistar-Kyoto strain. The DVL-1 sequence in the two strains was identical. Our results essentially exclude the DVL-1 gene as the cause for sensitivity to cerebral ischaemic insult in this rat model of stroke.

AB - A quantitative trait locus on chromosome 5 in the rat is linked to sensitivity to brain ischemia in the stroke-prone spontaneously hypertensive rat (SHRSP). The genes encoding atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) that map to this location have been excluded as candidate genes. We examined dishevelled-1 (DVL-1) as a further candidate gene. DVL-1 had not yet been identified in the rat, but Anp, Bnp, and DVL-1 map to the homologous regions of the rat chromosome 5 quantitative trait locus in both mice and man. Furthermore, DVL-1 is involved in the Notch signalling system, which plays a role in the disorder cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, the symptoms of which include ischaemic stroke. We show with radiation hybrid mapping that rat DVL-1 indeed maps to chromosome 5, where it is positioned immediately next to microsatellite marker D5Rat49. We sequenced the complete coding sequence and a large part of the intronic genomic sequence for the SHRSP strain and its reference Wistar-Kyoto strain. The DVL-1 sequence in the two strains was identical. Our results essentially exclude the DVL-1 gene as the cause for sensitivity to cerebral ischaemic insult in this rat model of stroke.

KW - genomic structure

KW - chromosomal location

KW - dishevelled

KW - hypertension

KW - rat

KW - SPONTANEOUSLY HYPERTENSIVE RATS

KW - NOTCH3 MUTATIONS

KW - POLARITY GENE

KW - PRONE

KW - SENSITIVITY

KW - CADASIL

KW - TRAIT

U2 - 10.1007/s100480000099

DO - 10.1007/s100480000099

M3 - Article

VL - 3

SP - 99

EP - 106

JO - Neurogenetics

JF - Neurogenetics

SN - 1364-6745

IS - 2

ER -