Massetolide A Biosynthesis in Pseudomonas fluorescens

Irene De Bruijn, M J D de Kock, P de Waard, T A van Beek, J M Raaijmakers

Research output: Contribution to journalArticle

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Abstract

Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes, designated massA, massB, and massC, spanning approximately 30 kb. Prediction of the nature and configuration of the amino acids by in silico analysis of adenylation and condensation domains of the NRPSs was consistent with the chemically determined structure of the peptide moiety of massetolide A. Structural analysis of massetolide A derivatives produced by SS101 indicated that most of the variations in the peptide moiety occur at amino acid positions 4 and 9. Regions flanking the mass genes contained several genes found in other Pseudomonas CLP biosynthesis clusters, which encode LuxR-type transcriptional regulators, ABC transporters, and an RND-like outer membrane protein. In contrast to most Pseudomonas CLP gene clusters known to date, the mass genes are not physically linked but are organized in two separate clusters, with massA disconnected from massB and massC. Quantitative real-time PCR analysis indicated that transcription of massC is strongly reduced when massB is mutated, suggesting that these two genes function in an operon, whereas transcription of massA is independent of massBC and vice versa. Massetolide A is produced in the early exponential growth phase, and biosynthesis appears not to be regulated by N-acylhomoserine lactone-based quorum sensing. Massetolide A production is essential in swarming motility of P. fluorescens SS101 and plays an important role in biofilm formation.
Original languageEnglish
Pages (from-to)2777-2789
Number of pages13
JournalJournal of Bacteriology
Volume190
Issue number8
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Pseudomonas fluorescens
Lipopeptides
Pseudomonas
Genes
Peptide Synthases
Amino Acids
Quorum Sensing
Peptides
ATP-Binding Cassette Transporters
Lactones
Operon
Biofilms
Multigene Family
Site-Directed Mutagenesis
Computer Simulation
Organism Cloning
Real-Time Polymerase Chain Reaction
Membrane Proteins
Anti-Bacterial Agents
Growth

Keywords

  • anti-bacterial agents
  • bacterial proteins
  • biofilms
  • cloning, molecular
  • DNA transposable elements
  • DNA, bacterial
  • gene expression profiling
  • gene expression regulation, bacterial
  • genetic complementation test
  • locomotion
  • multigene family
  • mutagenesis, insertional
  • operon
  • peptide synthases
  • peptides, cyclic
  • pseudomonas fluorescens
  • RNA, bacterial
  • RNA, messenger
  • sequence analysis, DNA

Cite this

De Bruijn, I., de Kock, M. J. D., de Waard, P., van Beek, T. A., & Raaijmakers, J. M. (2007). Massetolide A Biosynthesis in Pseudomonas fluorescens. Journal of Bacteriology, 190(8), 2777-2789. https://doi.org/10.1128/JB.01563-07

Massetolide A Biosynthesis in Pseudomonas fluorescens. / De Bruijn, Irene; de Kock, M J D; de Waard, P; van Beek, T A; Raaijmakers, J M.

In: Journal of Bacteriology, Vol. 190, No. 8, 11.2007, p. 2777-2789.

Research output: Contribution to journalArticle

De Bruijn, I, de Kock, MJD, de Waard, P, van Beek, TA & Raaijmakers, JM 2007, 'Massetolide A Biosynthesis in Pseudomonas fluorescens', Journal of Bacteriology, vol. 190, no. 8, pp. 2777-2789. https://doi.org/10.1128/JB.01563-07
De Bruijn I, de Kock MJD, de Waard P, van Beek TA, Raaijmakers JM. Massetolide A Biosynthesis in Pseudomonas fluorescens. Journal of Bacteriology. 2007 Nov;190(8):2777-2789. https://doi.org/10.1128/JB.01563-07
De Bruijn, Irene ; de Kock, M J D ; de Waard, P ; van Beek, T A ; Raaijmakers, J M. / Massetolide A Biosynthesis in Pseudomonas fluorescens. In: Journal of Bacteriology. 2007 ; Vol. 190, No. 8. pp. 2777-2789.
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