Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation

Paul M. Bartley*, Stephen E. Wright, Stephen W. Maley, Colin N. MacAldowie, Mintu Nath, Clare M. Hamilton, Frank Katzer, David Buxton, Elisabeth A. Innes

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The immune responses of pregnant cattle and their foetuses were examined following inoculation on day 70 of gestation either intravenously (iv) (group 1) or subcutaneously (sc) (group 2) with live NC1 strain tachyzoites or with Vero cells (control) (group 3). Peripheral blood mononuclear cell (PBMC) responses to Neospora antigen and foetal viability were assessed throughout the experiment. Two animals from each group were sacrificed at 14, 28, 42 and 56 days post inoculation (pi). At post mortem, maternal lymph nodes, spleen and PBMC and when possible foetal spleen, thymus and PBMC samples were collected for analysis. Inoculation with NC1 (iv and sc) lead to foetal deaths in all group 1 dams (6/6) and in 3/6 group 2 dams from day 28pi; statistically significant (p > 0.05) increases in cell-mediated immune (CMI) responses including antigen-specific cell proliferation and IFN-γ production as well as increased levels of IL-4, IL-10 and IL-12 were observed in challenged dams compared to the group 3 animals. Lymph node samples from the group 2 animals carrying live foetuses showed greater levels of cellular proliferation as well as significantly (p > 0.05) higher levels of IFN-γ compared to the dams in group 2 carrying dead foetuses. Foetal spleen, thymus and PBMC samples demonstrated cellular proliferation as well as IFN-γ, IL-4, IL-10 and IL-12 production following mitogenic stimulation with Con A from day 14pi (day 84 gestation) onwards. This study shows that the generation of robust peripheral and local maternal CMI responses (lymphoproliferation, IFN-γ) may inhibit the vertical transmission of the parasite.

Original languageEnglish
Article number38
Number of pages12
JournalVeterinary Research
Volume43
Issue number1
Early online date26 Apr 2012
DOIs
Publication statusPublished - 26 Apr 2012

Fingerprint

Neospora
Neospora caninum
mononuclear leukocytes
Blood Cells
Mothers
immune response
pregnancy
fetus
Pregnancy
cell proliferation
spleen
Fetus
Spleen
cattle
interleukin-12
Cell Proliferation
interleukin-4
Interleukin-12
interleukin-10
Interleukin-4

Keywords

  • Cattle
  • Early gestation
  • Maternal - foetal cellular immune
  • Neospora caninum

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation. / Bartley, Paul M.; Wright, Stephen E.; Maley, Stephen W.; MacAldowie, Colin N.; Nath, Mintu; Hamilton, Clare M.; Katzer, Frank; Buxton, David; Innes, Elisabeth A.

In: Veterinary Research, Vol. 43, No. 1, 38, 26.04.2012.

Research output: Contribution to journalArticle

Bartley, PM, Wright, SE, Maley, SW, MacAldowie, CN, Nath, M, Hamilton, CM, Katzer, F, Buxton, D & Innes, EA 2012, 'Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation', Veterinary Research, vol. 43, no. 1, 38. https://doi.org/10.1186/1297-9716-43-38
Bartley, Paul M. ; Wright, Stephen E. ; Maley, Stephen W. ; MacAldowie, Colin N. ; Nath, Mintu ; Hamilton, Clare M. ; Katzer, Frank ; Buxton, David ; Innes, Elisabeth A. / Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation. In: Veterinary Research. 2012 ; Vol. 43, No. 1.
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abstract = "The immune responses of pregnant cattle and their foetuses were examined following inoculation on day 70 of gestation either intravenously (iv) (group 1) or subcutaneously (sc) (group 2) with live NC1 strain tachyzoites or with Vero cells (control) (group 3). Peripheral blood mononuclear cell (PBMC) responses to Neospora antigen and foetal viability were assessed throughout the experiment. Two animals from each group were sacrificed at 14, 28, 42 and 56 days post inoculation (pi). At post mortem, maternal lymph nodes, spleen and PBMC and when possible foetal spleen, thymus and PBMC samples were collected for analysis. Inoculation with NC1 (iv and sc) lead to foetal deaths in all group 1 dams (6/6) and in 3/6 group 2 dams from day 28pi; statistically significant (p > 0.05) increases in cell-mediated immune (CMI) responses including antigen-specific cell proliferation and IFN-γ production as well as increased levels of IL-4, IL-10 and IL-12 were observed in challenged dams compared to the group 3 animals. Lymph node samples from the group 2 animals carrying live foetuses showed greater levels of cellular proliferation as well as significantly (p > 0.05) higher levels of IFN-γ compared to the dams in group 2 carrying dead foetuses. Foetal spleen, thymus and PBMC samples demonstrated cellular proliferation as well as IFN-γ, IL-4, IL-10 and IL-12 production following mitogenic stimulation with Con A from day 14pi (day 84 gestation) onwards. This study shows that the generation of robust peripheral and local maternal CMI responses (lymphoproliferation, IFN-γ) may inhibit the vertical transmission of the parasite.",
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AU - Innes, Elisabeth A.

N1 - Acknowledgements The authors would like to acknowledge the Scottish Government Rural and Environmental Research and Analysis Directorate (RERAD) for funding this study. The rBo IL-4, rBo IL-10 and rOvIL-12 were provided by Prof. Gary Entrican and Sean Wattegedera (Moredun Research Institute, Edinburgh, UK, (RERAD funded)). The recombinant bovine IFN-γ was kindly supplied by Dr. Steven Jones, (Pfizer Animal Health, Parkville, Australia).

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N2 - The immune responses of pregnant cattle and their foetuses were examined following inoculation on day 70 of gestation either intravenously (iv) (group 1) or subcutaneously (sc) (group 2) with live NC1 strain tachyzoites or with Vero cells (control) (group 3). Peripheral blood mononuclear cell (PBMC) responses to Neospora antigen and foetal viability were assessed throughout the experiment. Two animals from each group were sacrificed at 14, 28, 42 and 56 days post inoculation (pi). At post mortem, maternal lymph nodes, spleen and PBMC and when possible foetal spleen, thymus and PBMC samples were collected for analysis. Inoculation with NC1 (iv and sc) lead to foetal deaths in all group 1 dams (6/6) and in 3/6 group 2 dams from day 28pi; statistically significant (p > 0.05) increases in cell-mediated immune (CMI) responses including antigen-specific cell proliferation and IFN-γ production as well as increased levels of IL-4, IL-10 and IL-12 were observed in challenged dams compared to the group 3 animals. Lymph node samples from the group 2 animals carrying live foetuses showed greater levels of cellular proliferation as well as significantly (p > 0.05) higher levels of IFN-γ compared to the dams in group 2 carrying dead foetuses. Foetal spleen, thymus and PBMC samples demonstrated cellular proliferation as well as IFN-γ, IL-4, IL-10 and IL-12 production following mitogenic stimulation with Con A from day 14pi (day 84 gestation) onwards. This study shows that the generation of robust peripheral and local maternal CMI responses (lymphoproliferation, IFN-γ) may inhibit the vertical transmission of the parasite.

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