Maternal vitamin D and E intakes during early pregnancy are associated with airway epithelial cell responses in neonates

David R Miller, Steve W Turner, Daniella Spiteri-Cornish, Alison R Scaife, Peter J Danielian, Graham S Devereux, Garry M Walsh

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Abstract

BACKGROUND: Antenatal factors including maternal diet may predispose to airway disease, possibly by impacting on fetal airway development.

OBJECTIVE: This cohort study tested the hypothesis that maternal vitamin D and E status in early pregnancy is associated with airway epithelial cell (AEC) responses in new born infants and examined constitutive and TNFα/IL-1β, house dust mite (HDM) extract or lipopolysaccharide (LPS) stimulated neonatal AEC responses in vitro.

METHODS: Maternal dietary vitamin D and E intakes, (plasma 25[OH]D3 or α-tocopherol) were characterised at 10-12 weeks gestation. Neonatal nasal AEC were collected soon after birth and cultured to tertiary passage. Constitutive and stimulated - TNFα/IL-1β, HDM extract or LPS - secretory responses (VEGF, RANTES, MCP-1, IL-17A, IFN-γ, GM-CSF, eotaxin, MIP1-α, MIP1-β, ICAM, IL-6, IL-8, IL-10, TNF) in 139 AEC cultures were quantified.

RESULTS: AEC mediator release was greater following TNF-α/IL-1β, HDM or LPS stimulation compared to constitutive release. Increased maternal dietary vitamin D was associated with significant increases in IL-10 release by AEC after stimulation with TNF-α/IL-1β (p=0.024) or HDM (p=0.049). Maternal plasma α-tocopherol at 10-12 weeks gestation was positively associated with MIP1α (Spearman's rho 0.242, p=0.009) and IL-3 (ρ 0.189, p=0.043) responses after TNF-α/IL-1β stimulation and negatively associated with TNF (ρ -0.404, p=0.011) and MIP1β (ρ -0.322, p=0.046) responses after LPS stimulation.

DISCUSSION: Neonatal AEC respond to pro-inflammatory and allergenic stimuli in vitro demonstrating their potential to function as components of the innate immune response. Our findings suggest that associations exist between maternal micronutrient intake during early pregnancy and aspects of stimulated neonatal airway epithelial cell secretory function that may in turn impact on the development of asthma and/or allergic rhinitis in later life. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)920-927
Number of pages8
JournalClinical & experimental allergy
Volume45
Issue number5
Early online date16 Apr 2015
DOIs
Publication statusPublished - May 2015

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Vitamin E
Vitamin D
Epithelial Cells
Mothers
Newborn Infant
Pregnancy
Pyroglyphidae
Interleukin-1
Lipopolysaccharides
Tocopherols
Interleukin-10
Chemokine CCL5
Interleukin-17
Micronutrients
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Fetal Development
Interleukin-8
Nose
Innate Immunity

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Maternal vitamin D and E intakes during early pregnancy are associated with airway epithelial cell responses in neonates. / Miller, David R; Turner, Steve W; Spiteri-Cornish, Daniella; Scaife, Alison R; Danielian, Peter J; Devereux, Graham S; Walsh, Garry M.

In: Clinical & experimental allergy, Vol. 45, No. 5, 05.2015, p. 920-927.

Research output: Contribution to journalArticle

Miller, David R ; Turner, Steve W ; Spiteri-Cornish, Daniella ; Scaife, Alison R ; Danielian, Peter J ; Devereux, Graham S ; Walsh, Garry M. / Maternal vitamin D and E intakes during early pregnancy are associated with airway epithelial cell responses in neonates. In: Clinical & experimental allergy. 2015 ; Vol. 45, No. 5. pp. 920-927.
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abstract = "BACKGROUND: Antenatal factors including maternal diet may predispose to airway disease, possibly by impacting on fetal airway development.OBJECTIVE: This cohort study tested the hypothesis that maternal vitamin D and E status in early pregnancy is associated with airway epithelial cell (AEC) responses in new born infants and examined constitutive and TNFα/IL-1β, house dust mite (HDM) extract or lipopolysaccharide (LPS) stimulated neonatal AEC responses in vitro.METHODS: Maternal dietary vitamin D and E intakes, (plasma 25[OH]D3 or α-tocopherol) were characterised at 10-12 weeks gestation. Neonatal nasal AEC were collected soon after birth and cultured to tertiary passage. Constitutive and stimulated - TNFα/IL-1β, HDM extract or LPS - secretory responses (VEGF, RANTES, MCP-1, IL-17A, IFN-γ, GM-CSF, eotaxin, MIP1-α, MIP1-β, ICAM, IL-6, IL-8, IL-10, TNF) in 139 AEC cultures were quantified.RESULTS: AEC mediator release was greater following TNF-α/IL-1β, HDM or LPS stimulation compared to constitutive release. Increased maternal dietary vitamin D was associated with significant increases in IL-10 release by AEC after stimulation with TNF-α/IL-1β (p=0.024) or HDM (p=0.049). Maternal plasma α-tocopherol at 10-12 weeks gestation was positively associated with MIP1α (Spearman's rho 0.242, p=0.009) and IL-3 (ρ 0.189, p=0.043) responses after TNF-α/IL-1β stimulation and negatively associated with TNF (ρ -0.404, p=0.011) and MIP1β (ρ -0.322, p=0.046) responses after LPS stimulation.DISCUSSION: Neonatal AEC respond to pro-inflammatory and allergenic stimuli in vitro demonstrating their potential to function as components of the innate immune response. Our findings suggest that associations exist between maternal micronutrient intake during early pregnancy and aspects of stimulated neonatal airway epithelial cell secretory function that may in turn impact on the development of asthma and/or allergic rhinitis in later life. This article is protected by copyright. All rights reserved.",
author = "Miller, {David R} and Turner, {Steve W} and Daniella Spiteri-Cornish and Scaife, {Alison R} and Danielian, {Peter J} and Devereux, {Graham S} and Walsh, {Garry M}",
note = "This article is protected by copyright. All rights reserved. ACKNOWLEDGEMENTS The authors would like to thank the children who participated in this study and their parents. We would also like to express our gratitude to staff at Aberdeen Maternity Hospital. This study was funded by the Chief Scientist Office, Edinburgh, UK (Clinical Academic Fellowship to DM).",
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T1 - Maternal vitamin D and E intakes during early pregnancy are associated with airway epithelial cell responses in neonates

AU - Miller, David R

AU - Turner, Steve W

AU - Spiteri-Cornish, Daniella

AU - Scaife, Alison R

AU - Danielian, Peter J

AU - Devereux, Graham S

AU - Walsh, Garry M

N1 - This article is protected by copyright. All rights reserved. ACKNOWLEDGEMENTS The authors would like to thank the children who participated in this study and their parents. We would also like to express our gratitude to staff at Aberdeen Maternity Hospital. This study was funded by the Chief Scientist Office, Edinburgh, UK (Clinical Academic Fellowship to DM).

PY - 2015/5

Y1 - 2015/5

N2 - BACKGROUND: Antenatal factors including maternal diet may predispose to airway disease, possibly by impacting on fetal airway development.OBJECTIVE: This cohort study tested the hypothesis that maternal vitamin D and E status in early pregnancy is associated with airway epithelial cell (AEC) responses in new born infants and examined constitutive and TNFα/IL-1β, house dust mite (HDM) extract or lipopolysaccharide (LPS) stimulated neonatal AEC responses in vitro.METHODS: Maternal dietary vitamin D and E intakes, (plasma 25[OH]D3 or α-tocopherol) were characterised at 10-12 weeks gestation. Neonatal nasal AEC were collected soon after birth and cultured to tertiary passage. Constitutive and stimulated - TNFα/IL-1β, HDM extract or LPS - secretory responses (VEGF, RANTES, MCP-1, IL-17A, IFN-γ, GM-CSF, eotaxin, MIP1-α, MIP1-β, ICAM, IL-6, IL-8, IL-10, TNF) in 139 AEC cultures were quantified.RESULTS: AEC mediator release was greater following TNF-α/IL-1β, HDM or LPS stimulation compared to constitutive release. Increased maternal dietary vitamin D was associated with significant increases in IL-10 release by AEC after stimulation with TNF-α/IL-1β (p=0.024) or HDM (p=0.049). Maternal plasma α-tocopherol at 10-12 weeks gestation was positively associated with MIP1α (Spearman's rho 0.242, p=0.009) and IL-3 (ρ 0.189, p=0.043) responses after TNF-α/IL-1β stimulation and negatively associated with TNF (ρ -0.404, p=0.011) and MIP1β (ρ -0.322, p=0.046) responses after LPS stimulation.DISCUSSION: Neonatal AEC respond to pro-inflammatory and allergenic stimuli in vitro demonstrating their potential to function as components of the innate immune response. Our findings suggest that associations exist between maternal micronutrient intake during early pregnancy and aspects of stimulated neonatal airway epithelial cell secretory function that may in turn impact on the development of asthma and/or allergic rhinitis in later life. This article is protected by copyright. All rights reserved.

AB - BACKGROUND: Antenatal factors including maternal diet may predispose to airway disease, possibly by impacting on fetal airway development.OBJECTIVE: This cohort study tested the hypothesis that maternal vitamin D and E status in early pregnancy is associated with airway epithelial cell (AEC) responses in new born infants and examined constitutive and TNFα/IL-1β, house dust mite (HDM) extract or lipopolysaccharide (LPS) stimulated neonatal AEC responses in vitro.METHODS: Maternal dietary vitamin D and E intakes, (plasma 25[OH]D3 or α-tocopherol) were characterised at 10-12 weeks gestation. Neonatal nasal AEC were collected soon after birth and cultured to tertiary passage. Constitutive and stimulated - TNFα/IL-1β, HDM extract or LPS - secretory responses (VEGF, RANTES, MCP-1, IL-17A, IFN-γ, GM-CSF, eotaxin, MIP1-α, MIP1-β, ICAM, IL-6, IL-8, IL-10, TNF) in 139 AEC cultures were quantified.RESULTS: AEC mediator release was greater following TNF-α/IL-1β, HDM or LPS stimulation compared to constitutive release. Increased maternal dietary vitamin D was associated with significant increases in IL-10 release by AEC after stimulation with TNF-α/IL-1β (p=0.024) or HDM (p=0.049). Maternal plasma α-tocopherol at 10-12 weeks gestation was positively associated with MIP1α (Spearman's rho 0.242, p=0.009) and IL-3 (ρ 0.189, p=0.043) responses after TNF-α/IL-1β stimulation and negatively associated with TNF (ρ -0.404, p=0.011) and MIP1β (ρ -0.322, p=0.046) responses after LPS stimulation.DISCUSSION: Neonatal AEC respond to pro-inflammatory and allergenic stimuli in vitro demonstrating their potential to function as components of the innate immune response. Our findings suggest that associations exist between maternal micronutrient intake during early pregnancy and aspects of stimulated neonatal airway epithelial cell secretory function that may in turn impact on the development of asthma and/or allergic rhinitis in later life. This article is protected by copyright. All rights reserved.

U2 - 10.1111/cea.12490

DO - 10.1111/cea.12490

M3 - Article

VL - 45

SP - 920

EP - 927

JO - Clinical & experimental allergy

JF - Clinical & experimental allergy

SN - 0954-7894

IS - 5

ER -