Mathematical modelling of the diurnal regulation of the MEP pathway in Arabidopsis

Alexandra Pokhilko, Jordi Bou-Torrent, Pablo Pulido, Manuel Rodríguez-Concepción, Oliver Ebenhöh

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Isoprenoid molecules are essential elements of plant metabolism. Many important plant isoprenoids, such as chlorophylls, carotenoids, tocopherols, prenylated quinones and hormones are synthesised in chloroplasts via the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Here we develop a mathematical model of diurnal regulation of the MEP pathway in Arabidopsis thaliana. We used both experimental and theoretical approaches to integrate mechanisms potentially involved in the diurnal control of the pathway. Our data show that flux through the MEP pathway is accelerated in light due to the photosynthesis-dependent supply of metabolic substrates of the pathway and the transcriptional regulation of key biosynthetic genes by the circadian clock. We also demonstrate that feedback regulation of both the activity and the abundance of the first enzyme of the MEP pathway (1-deoxy-d-xylulose 5-phosphate synthase, DXS) by pathway products stabilizes the flux against changes in substrate supply and adjusts the flux according to product demand under normal growth conditions. These data illustrate the central relevance of photosynthesis, the circadian clock and feedback control of DXS for the diurnal regulation of the MEP pathway.

Original languageEnglish
Pages (from-to)1075-1085
Number of pages11
JournalNew Phytologist
Volume206
Issue number3
Early online date16 Jan 2015
DOIs
Publication statusPublished - May 2015

Fingerprint

erythritol
Arabidopsis
mathematical models
phosphates
Circadian Clocks
Terpenes
Photosynthesis
isoprenoids
circadian rhythm
Quinones
photosynthesis
Tocopherols
xylulose
Chloroplasts
Carotenoids
Chlorophyll
Metabolic Networks and Pathways
quinones
tocopherols
Theoretical Models

Keywords

  • 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway
  • arabidopsis thaliana
  • isoprenoids
  • mathematical modelling
  • plant metabolism
  • systems biology
  • whole plant physiology

Cite this

Pokhilko, A., Bou-Torrent, J., Pulido, P., Rodríguez-Concepción, M., & Ebenhöh, O. (2015). Mathematical modelling of the diurnal regulation of the MEP pathway in Arabidopsis. New Phytologist, 206(3), 1075-1085. https://doi.org/10.1111/nph.13258

Mathematical modelling of the diurnal regulation of the MEP pathway in Arabidopsis. / Pokhilko, Alexandra; Bou-Torrent, Jordi; Pulido, Pablo; Rodríguez-Concepción, Manuel; Ebenhöh, Oliver.

In: New Phytologist, Vol. 206, No. 3, 05.2015, p. 1075-1085.

Research output: Contribution to journalArticle

Pokhilko, A, Bou-Torrent, J, Pulido, P, Rodríguez-Concepción, M & Ebenhöh, O 2015, 'Mathematical modelling of the diurnal regulation of the MEP pathway in Arabidopsis', New Phytologist, vol. 206, no. 3, pp. 1075-1085. https://doi.org/10.1111/nph.13258
Pokhilko A, Bou-Torrent J, Pulido P, Rodríguez-Concepción M, Ebenhöh O. Mathematical modelling of the diurnal regulation of the MEP pathway in Arabidopsis. New Phytologist. 2015 May;206(3):1075-1085. https://doi.org/10.1111/nph.13258
Pokhilko, Alexandra ; Bou-Torrent, Jordi ; Pulido, Pablo ; Rodríguez-Concepción, Manuel ; Ebenhöh, Oliver. / Mathematical modelling of the diurnal regulation of the MEP pathway in Arabidopsis. In: New Phytologist. 2015 ; Vol. 206, No. 3. pp. 1075-1085.
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abstract = "Isoprenoid molecules are essential elements of plant metabolism. Many important plant isoprenoids, such as chlorophylls, carotenoids, tocopherols, prenylated quinones and hormones are synthesised in chloroplasts via the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Here we develop a mathematical model of diurnal regulation of the MEP pathway in Arabidopsis thaliana. We used both experimental and theoretical approaches to integrate mechanisms potentially involved in the diurnal control of the pathway. Our data show that flux through the MEP pathway is accelerated in light due to the photosynthesis-dependent supply of metabolic substrates of the pathway and the transcriptional regulation of key biosynthetic genes by the circadian clock. We also demonstrate that feedback regulation of both the activity and the abundance of the first enzyme of the MEP pathway (1-deoxy-d-xylulose 5-phosphate synthase, DXS) by pathway products stabilizes the flux against changes in substrate supply and adjusts the flux according to product demand under normal growth conditions. These data illustrate the central relevance of photosynthesis, the circadian clock and feedback control of DXS for the diurnal regulation of the MEP pathway.",
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