The (13)C-labelled Na-bicarbonate technique uses stable isotopes to measure energy expenditure in birds. After administration, the isotopes reach equilibrium within the body's bicarbonate pools at a fast rate due to the small size of the bicarbonate pool in relation to CO(2) flux. This technique is therefore ideal for measuring energy expenditure over short-term activities. The major advantage of this technique is that it can be applied without the animal having to wear a respirometry mask or being enclosed in a respirometry chamber. Despite the technique's suitability for use in birds and other animals, there have been few studies that have used it to date and so its potential is not fully understood. Here we discuss the methodology and review previous applications.
|Number of pages||6|
|Journal||Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology|
|Early online date||25 May 2010|
|Publication status||Published - Mar 2011|
- labelled Na-bicarbonate technique
- energy expenditure