Abstract
The underlying mechanism of normal lung organogenesis is not well understood. An increasing number of studies are demonstrating that extracellular vesicles (EVs) play critical roles in organ development by delivering microRNAs (miRNA) to neighboring and distant cells. miRNAs are important for fetal lung growth; however, the role of miRNA–EVs (miRNAs packaged inside the EVs) during fetal lung development is unexplored. The aim of this study was to examine the expression of miRNA–EVs in MLE-12, a murine lung epithelial cell line subjected to mechanical stretch in vitro with the long-term goal to investigate their potential role in the fetal lung development. Both cyclic and continuous mechanical stretch regulate miRNA differentially in EVs released from MLE-12 and intracellularly, demonstrating that mechanical signals regulate the expression of miRNA–EVs in lung epithelial cells. These results provide a proof-of-concept for the potential role that miRNA–EVs could play in the development of fetal lung.
Original language | English |
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Pages (from-to) | 8210-8223 |
Number of pages | 14 |
Journal | Journal of Cellular Physiology |
Volume | 235 |
Issue number | 11 |
Early online date | 22 Jan 2020 |
DOIs | |
Publication status | Published - Nov 2020 |
Keywords
- mechanical stretch
- extracellular vesicles
- MiRNA
- MLE-12
- miRNA
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Profiles
-
Rasha Abu Eid
- School of Medicine, Medical Sciences & Nutrition, Dental Education - Senior Lecturer
- Clinical Medicine
- Institute of Medical Sciences
Person: Academic