Mechanical stretch regulates the expression of specific miRNA in extracellular vesicles released from lung epithelial cells

Tanbir Najrana; Anshu Mahadeo; Rasha Abu Eid; Elena Kreienberg; Victoria Schulte; Alper Uzun; Christoph Schorl; Laura Goldberg; Peter Quesenberry; Juan Sanchez-Esteban

doi:10.1002/jcp.29476

Mechanical stretch regulates the expression of specific miRNA in extracellular vesicles released from lung epithelial cells

Tanbir Najrana^* (Corresponding Author), Anshu Mahadeo, Rasha Abu Eid, Elena Kreienberg, Victoria Schulte, Alper Uzun, Christoph Schorl, Laura Goldberg, Peter Quesenberry, Juan Sanchez-Esteban

^*Corresponding author for this work

Dental Education

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Abstract

The underlying mechanism of normal lung organogenesis is not well understood. An increasing number of studies are demonstrating that extracellular vesicles (EVs) play critical roles in organ development by delivering microRNAs (miRNA) to neighboring and distant cells. miRNAs are important for fetal lung growth; however, the role of miRNA–EVs (miRNAs packaged inside the EVs) during fetal lung development is unexplored. The aim of this study was to examine the expression of miRNA–EVs in MLE-12, a murine lung epithelial cell line subjected to mechanical stretch in vitro with the long-term goal to investigate their potential role in the fetal lung development. Both cyclic and continuous mechanical stretch regulate miRNA differentially in EVs released from MLE-12 and intracellularly, demonstrating that mechanical signals regulate the expression of miRNA–EVs in lung epithelial cells. These results provide a proof-of-concept for the potential role that miRNA–EVs could play in the development of fetal lung.

Original language	English
Pages (from-to)	8210-8223
Number of pages	14
Journal	Journal of Cellular Physiology
Volume	235
Issue number	11
Early online date	22 Jan 2020
DOIs	https://doi.org/10.1002/jcp.29476
Publication status	Published - Nov 2020

Bibliographical note

Acknowledgments
This work was supported from the National Institute of Health (NIGMS grant Number P30GM114750 & P30GM103410, NCRR grant Numbers P30RR031153, P20RR018728 & S10RR02763); National Science Foundation (EPSCoR grant No 0554548); Oh–Zopfi for Perinatal Research Award, Women & Infants Hospital of Rhode Island. We thank Brenda Vecchio for her support in manuscript formatting and Quanfu Mao for his support to use the instruments.

Keywords

mechanical stretch
extracellular vesicles
MiRNA
MLE-12
miRNA

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Najrana_et_al_JoCP_MechanicalStretchRegulates_AAM
This is the peer reviewed version of the following article: Najrana, T, Mahadeo, A, Abu‐Eid, R, et al. Mechanical stretch regulates the expression of specific miRNA in extracellular vesicles released from lung epithelial cells. J Cell Physiol. 2020; 235: 8210– 8223. https://doi.org/10.1002/jcp.29476, which has been published in final form at https://doi.org/10.1002/jcp.29476. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Accepted author manuscript, 1.58 MBLicence: Unspecified

Cite this

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title = "Mechanical stretch regulates the expression of specific miRNA in extracellular vesicles released from lung epithelial cells",

abstract = "The underlying mechanism of normal lung organogenesis is not well understood. An increasing number of studies are demonstrating that extracellular vesicles (EVs) play critical roles in organ development by delivering microRNAs (miRNA) to neighboring and distant cells. miRNAs are important for fetal lung growth; however, the role of miRNA–EVs (miRNAs packaged inside the EVs) during fetal lung development is unexplored. The aim of this study was to examine the expression of miRNA–EVs in MLE-12, a murine lung epithelial cell line subjected to mechanical stretch in vitro with the long-term goal to investigate their potential role in the fetal lung development. Both cyclic and continuous mechanical stretch regulate miRNA differentially in EVs released from MLE-12 and intracellularly, demonstrating that mechanical signals regulate the expression of miRNA–EVs in lung epithelial cells. These results provide a proof-of-concept for the potential role that miRNA–EVs could play in the development of fetal lung.",

keywords = "mechanical stretch, extracellular vesicles, MiRNA, MLE-12, miRNA",

author = "Tanbir Najrana and Anshu Mahadeo and {Abu Eid}, Rasha and Elena Kreienberg and Victoria Schulte and Alper Uzun and Christoph Schorl and Laura Goldberg and Peter Quesenberry and Juan Sanchez-Esteban",

note = "Acknowledgments This work was supported from the National Institute of Health (NIGMS grant Number P30GM114750 & P30GM103410, NCRR grant Numbers P30RR031153, P20RR018728 & S10RR02763); National Science Foundation (EPSCoR grant No 0554548); Oh–Zopfi for Perinatal Research Award, Women & Infants Hospital of Rhode Island. We thank Brenda Vecchio for her support in manuscript formatting and Quanfu Mao for his support to use the instruments. ",

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Mechanical stretch regulates the expression of specific miRNA in extracellular vesicles released from lung epithelial cells

Abstract

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Rasha Abu Eid

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Mechanical stretch regulates the expression of specific miRNA in extracellular vesicles released from lung epithelial cells

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Bibliographical note

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Rasha Abu Eid

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