Mechanical Stretch regulates the expression of specific miRNAs in extracellular vesicles released from lung epithelial cells

Tanbir Najrana* (Corresponding Author), Anshu Mahadeo, Rasha Abu Eid, Elena Kreienberg, Victoria Schulte, Alper Uzun, Christoph Schorl, Laura Goldberg, Peter Quesenberry, Juan Sanchez-Esteban

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The underlying mechanism of normal lung organogenesis is not well understood. An increasing number of studies are demonstrating that extracellular vesicles (EVs) play critical roles in organ development by delivering microRNAs (miRNA) to neighboring and distant cells. miRNAs are important for fetal lung growth; however, the role of miRNA-EVs (miRNAs packaged inside the EVs) during fetal lung development is unexplored. The aim of this study was to examine the expression of miRNA-EVs in MLE-12, a murine lung epithelial cell line subjected to mechanical stretch in vitro with the long-term goal to investigate their potential role in fetal lung development. Both cyclic and continuous mechanical stretch regulate miRNA differentially in EVs released from MLE-12 and intracellularly, demonstrating that mechanical signals regulate the expression of miRNA-EVs in lung epithelial cells. These results provide a proof-of-concept for the potential role that miRNA-EVs could play in the development of fetal lung.
Original languageEnglish
JournalJournal of Cellular Physiology
Early online date22 Jan 2020
DOIs
Publication statusE-pub ahead of print - 22 Jan 2020

Fingerprint

MicroRNAs
Epithelial Cells
Lung
Fetal Development
Maximum likelihood estimation
Organogenesis
Extracellular Vesicles
Cell Line

Keywords

  • mechanical stretch
  • extracellular vesicles
  • MiRNA
  • MLE-12
  • miRNA

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Mechanical Stretch regulates the expression of specific miRNAs in extracellular vesicles released from lung epithelial cells. / Najrana, Tanbir (Corresponding Author); Mahadeo, Anshu; Abu Eid, Rasha; Kreienberg, Elena; Schulte, Victoria; Uzun, Alper; Schorl, Christoph; Goldberg, Laura; Quesenberry, Peter; Sanchez-Esteban, Juan.

In: Journal of Cellular Physiology, 22.01.2020.

Research output: Contribution to journalArticle

Najrana, Tanbir ; Mahadeo, Anshu ; Abu Eid, Rasha ; Kreienberg, Elena ; Schulte, Victoria ; Uzun, Alper ; Schorl, Christoph ; Goldberg, Laura ; Quesenberry, Peter ; Sanchez-Esteban, Juan. / Mechanical Stretch regulates the expression of specific miRNAs in extracellular vesicles released from lung epithelial cells. In: Journal of Cellular Physiology. 2020.
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title = "Mechanical Stretch regulates the expression of specific miRNAs in extracellular vesicles released from lung epithelial cells",
abstract = "The underlying mechanism of normal lung organogenesis is not well understood. An increasing number of studies are demonstrating that extracellular vesicles (EVs) play critical roles in organ development by delivering microRNAs (miRNA) to neighboring and distant cells. miRNAs are important for fetal lung growth; however, the role of miRNA-EVs (miRNAs packaged inside the EVs) during fetal lung development is unexplored. The aim of this study was to examine the expression of miRNA-EVs in MLE-12, a murine lung epithelial cell line subjected to mechanical stretch in vitro with the long-term goal to investigate their potential role in fetal lung development. Both cyclic and continuous mechanical stretch regulate miRNA differentially in EVs released from MLE-12 and intracellularly, demonstrating that mechanical signals regulate the expression of miRNA-EVs in lung epithelial cells. These results provide a proof-of-concept for the potential role that miRNA-EVs could play in the development of fetal lung.",
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author = "Tanbir Najrana and Anshu Mahadeo and {Abu Eid}, Rasha and Elena Kreienberg and Victoria Schulte and Alper Uzun and Christoph Schorl and Laura Goldberg and Peter Quesenberry and Juan Sanchez-Esteban",
note = "Acknowledgments This work was supported from the National Institute of Health (NIGMS grant Number P30GM114750 & P30GM103410, NCRR grant Numbers P30RR031153, P20RR018728 & S10RR02763); National Science Foundation (EPSCoR grant No 0554548); Oh–Zopfi for Perinatal Research Award, Women & Infants Hospital of Rhode Island. We thank Brenda Vecchio for her support in manuscript formatting and Quanfu Mao for his support to use the instruments.",
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N1 - Acknowledgments This work was supported from the National Institute of Health (NIGMS grant Number P30GM114750 & P30GM103410, NCRR grant Numbers P30RR031153, P20RR018728 & S10RR02763); National Science Foundation (EPSCoR grant No 0554548); Oh–Zopfi for Perinatal Research Award, Women & Infants Hospital of Rhode Island. We thank Brenda Vecchio for her support in manuscript formatting and Quanfu Mao for his support to use the instruments.

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AB - The underlying mechanism of normal lung organogenesis is not well understood. An increasing number of studies are demonstrating that extracellular vesicles (EVs) play critical roles in organ development by delivering microRNAs (miRNA) to neighboring and distant cells. miRNAs are important for fetal lung growth; however, the role of miRNA-EVs (miRNAs packaged inside the EVs) during fetal lung development is unexplored. The aim of this study was to examine the expression of miRNA-EVs in MLE-12, a murine lung epithelial cell line subjected to mechanical stretch in vitro with the long-term goal to investigate their potential role in fetal lung development. Both cyclic and continuous mechanical stretch regulate miRNA differentially in EVs released from MLE-12 and intracellularly, demonstrating that mechanical signals regulate the expression of miRNA-EVs in lung epithelial cells. These results provide a proof-of-concept for the potential role that miRNA-EVs could play in the development of fetal lung.

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