Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62)

Hai Deng, Stephen A McMahon, Alessandra S Eustaquio, Bradley S Moore, James H Naismith, David O'Hagan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The substrate analogue S-adenosyl-l-homocysteine (SAH) was co-crystallised with SAM hydroxide adenosyltransferase from Pyrococcus horikoshii. Of the two active site water molecules one appears to be structural and the other is a candidate for nucleophilic attack, to become the C5′ adenosyl hydroxyl group. The data support a mechanism in which the Arg–Asp ion pair is important for positioning both water molecules.
Original languageEnglish
Pages (from-to)2455-2459
Number of pages5
JournalChemBioChem
Volume10
Issue number15
Early online date8 Sep 2009
DOIs
Publication statusPublished - 12 Oct 2009

Fingerprint

Pyrococcus horikoshii
Chemical activation
Molecules
Water
Homocysteine
Hydroxyl Radical
Catalytic Domain
Ions
Substrates
hydroxide ion

Keywords

  • biosynthesis
  • catalysis
  • enzyme catalysis
  • enzymes

Cite this

Deng, H., McMahon, S. A., Eustaquio, A. S., Moore, B. S., Naismith, J. H., & O'Hagan, D. (2009). Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62). ChemBioChem, 10(15), 2455-2459. https://doi.org/10.1002/cbic.200900369

Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62). / Deng, Hai; McMahon, Stephen A; Eustaquio, Alessandra S; Moore, Bradley S; Naismith, James H; O'Hagan, David.

In: ChemBioChem, Vol. 10, No. 15, 12.10.2009, p. 2455-2459.

Research output: Contribution to journalArticle

Deng, H, McMahon, SA, Eustaquio, AS, Moore, BS, Naismith, JH & O'Hagan, D 2009, 'Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62)', ChemBioChem, vol. 10, no. 15, pp. 2455-2459. https://doi.org/10.1002/cbic.200900369
Deng H, McMahon SA, Eustaquio AS, Moore BS, Naismith JH, O'Hagan D. Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62). ChemBioChem. 2009 Oct 12;10(15):2455-2459. https://doi.org/10.1002/cbic.200900369
Deng, Hai ; McMahon, Stephen A ; Eustaquio, Alessandra S ; Moore, Bradley S ; Naismith, James H ; O'Hagan, David. / Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62). In: ChemBioChem. 2009 ; Vol. 10, No. 15. pp. 2455-2459.
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