Melatonin receptor mRNA expression in Xenopus oocytes: Inhibition of G-protein-activated response

S P FRASER, Perry Barrett, M B A DJAMGOZ, Peter John Morgan

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Abstract

Melatonin is the major endocrine product of the pineal gland in the mammalian brain and plays a variety of roles in photoperiodic functions. In order to investigate melatonin receptors, poly(A)+ RNA was extracted from pars tuberalis of the ovine pituitary and injected into oocytes of Xenopus laevis. After 3-5 days of incubation, functional melatonin receptors were expressed. Receptors were revealed by their inhibitory effect upon oscillatory currents resulting from AlF4-induced activation of G-proteins in the oocyte membrane under voltage clamp conditions. The effect of melatonin was dose-dependent, non-desensitizing and was not observed in uninjected oocytes.

Original languageEnglish
Pages (from-to)242-245
Number of pages4
JournalNeuroscience Letters
Volume124
Issue number2
DOIs
Publication statusPublished - 1 Apr 1991

Keywords

  • melatonin
  • receptor
  • G-protein
  • inositol trisphosphate
  • Xenopus oocyte
  • aluminium fluoride
  • messenger RNA
  • Pars Tuberalis
  • Ovine Pars Tuberalis
  • acetylcholine-receptors
  • membrane
  • adenylate-cyclase
  • fluoride
  • aluminium
  • pineal
  • brain
  • sites

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