Melatonin receptors in the human fetal kidney: 2-[I-125]iodomelatonin binding sites correlated with expression of Mel1a and Mel1b receptor genes

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Abstract

Melatonin receptors in the human fetal kidney were identified and characterized by quantitative in vitro autoradiography using the melatonin agonist, 2-[I-125]iodomelatonin. Specific binding was localized to cells in the nephrogenic region at the outer perimeter of the developing kidney and was time-dependent, saturable and inhibited in the presence of guanosine 5'-O-(3-thiotriphosphate) indicative of a G protein-coupled receptor. Expression of the Mel1a and Mel1b melatonin receptors in human fetal kidney was determined using RT-PCR. In situ hybridization confirmed the localization of the Mel1a mRNA transcripts. A role for melatonin in development of the human fetal kidney is postulated.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalJournal of Endocrinology
Volume156
Issue number2
Publication statusPublished - Feb 1998

Keywords

  • photoperiod
  • localization
  • rhythm
  • sheep
  • fetus

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