Melatonin receptors in the human fetal kidney: 2-[I-125]iodomelatonin binding sites correlated with expression of Mel1a and Mel1b receptor genes

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Abstract

Melatonin receptors in the human fetal kidney were identified and characterized by quantitative in vitro autoradiography using the melatonin agonist, 2-[I-125]iodomelatonin. Specific binding was localized to cells in the nephrogenic region at the outer perimeter of the developing kidney and was time-dependent, saturable and inhibited in the presence of guanosine 5'-O-(3-thiotriphosphate) indicative of a G protein-coupled receptor. Expression of the Mel1a and Mel1b melatonin receptors in human fetal kidney was determined using RT-PCR. In situ hybridization confirmed the localization of the Mel1a mRNA transcripts. A role for melatonin in development of the human fetal kidney is postulated.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalJournal of Endocrinology
Volume156
Issue number2
Publication statusPublished - Feb 1998

Keywords

  • photoperiod
  • localization
  • rhythm
  • sheep
  • fetus

Cite this

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title = "Melatonin receptors in the human fetal kidney: 2-[I-125]iodomelatonin binding sites correlated with expression of Mel1a and Mel1b receptor genes",
abstract = "Melatonin receptors in the human fetal kidney were identified and characterized by quantitative in vitro autoradiography using the melatonin agonist, 2-[I-125]iodomelatonin. Specific binding was localized to cells in the nephrogenic region at the outer perimeter of the developing kidney and was time-dependent, saturable and inhibited in the presence of guanosine 5'-O-(3-thiotriphosphate) indicative of a G protein-coupled receptor. Expression of the Mel1a and Mel1b melatonin receptors in human fetal kidney was determined using RT-PCR. In situ hybridization confirmed the localization of the Mel1a mRNA transcripts. A role for melatonin in development of the human fetal kidney is postulated.",
keywords = "photoperiod, localization, rhythm, sheep, fetus",
author = "Janice Drew and Lynda Williams and Hannah, {L T} and Perry Barrett and David Abramovich and Morgan, {Peter John}",
year = "1998",
month = "2",
language = "English",
volume = "156",
pages = "261--267",
journal = "Journal of Endocrinology",
issn = "0022-0795",
publisher = "Society for Endocrinology",
number = "2",

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TY - JOUR

T1 - Melatonin receptors in the human fetal kidney: 2-[I-125]iodomelatonin binding sites correlated with expression of Mel1a and Mel1b receptor genes

AU - Drew, Janice

AU - Williams, Lynda

AU - Hannah, L T

AU - Barrett, Perry

AU - Abramovich, David

AU - Morgan, Peter John

PY - 1998/2

Y1 - 1998/2

N2 - Melatonin receptors in the human fetal kidney were identified and characterized by quantitative in vitro autoradiography using the melatonin agonist, 2-[I-125]iodomelatonin. Specific binding was localized to cells in the nephrogenic region at the outer perimeter of the developing kidney and was time-dependent, saturable and inhibited in the presence of guanosine 5'-O-(3-thiotriphosphate) indicative of a G protein-coupled receptor. Expression of the Mel1a and Mel1b melatonin receptors in human fetal kidney was determined using RT-PCR. In situ hybridization confirmed the localization of the Mel1a mRNA transcripts. A role for melatonin in development of the human fetal kidney is postulated.

AB - Melatonin receptors in the human fetal kidney were identified and characterized by quantitative in vitro autoradiography using the melatonin agonist, 2-[I-125]iodomelatonin. Specific binding was localized to cells in the nephrogenic region at the outer perimeter of the developing kidney and was time-dependent, saturable and inhibited in the presence of guanosine 5'-O-(3-thiotriphosphate) indicative of a G protein-coupled receptor. Expression of the Mel1a and Mel1b melatonin receptors in human fetal kidney was determined using RT-PCR. In situ hybridization confirmed the localization of the Mel1a mRNA transcripts. A role for melatonin in development of the human fetal kidney is postulated.

KW - photoperiod

KW - localization

KW - rhythm

KW - sheep

KW - fetus

M3 - Article

VL - 156

SP - 261

EP - 267

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

IS - 2

ER -