Melatonin receptors in the rat brain and pituitary

Lynda Williams, L T Hannah, M H Hastings, E S Maywood

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

2-(I-125)iodomelatonin binding has been mapped and characterized in the brain and pituitary of the male laboratory rat using quantitative in vitro autoradiography. Specific binding was defined as that completely displaced in the presence of 1 mu M melatonin. In the brain high levels of binding were localized over the suprachiasmatic nucleus (SCN), the area postrema (AP), and the spinal tract of the trigeminal nerve (Sp5). Lower densities of binding were found over the medial preoptic area (MPA), the septohypothalamic nuclei (SHy), the anterior hypothalamic area (AHA), the nuclei of the lateral olfactory tract (LOT), the paraventricular (PV), anteroventral (AV) and intermediodorsal (IMD) nuclei of the thalamus, the medial region of the lateral habenular (Lhb), the nuclei of the stria medullaris (SM), the basolateral (BL) and medial (ME) amygdaloid nuclei, the ventromedial nuclei (VMH), the arcuate nuclei (Are), the subiculum of the hippocampus (S) and the lateral mammillary nuclei (LM). High levels of binding were also present over the pars tuberalis of the pituitary (PT) and the anterior and posterior cerebral arteries (CA). In both neuronal and non-neuronal areas, specific binding was time dependent and partially reversible in the presence of 1 mu M melatonin. Binding was also saturable and of high affinity with dissociation constants (Kd) in the low picomolar range and was significantly inhibited in the presence of 10(-4)M guanosine 5'-0-(3-thiotriphosphate) (GTP gamma S) and 150 mM NaCl in all regions examined, indicating the presence of high affinity G-protein coupled melatonin receptors.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalJournal of Pineal Research
Volume19
Issue number4
Publication statusPublished - Nov 1995

Keywords

  • melatonin receptors
  • rat brain
  • pars tuberalis
  • G-protein
  • anterodorsal thalamic nuceli
  • guanine-nucleotides
  • invitro autoradiography
  • binding
  • localization
  • modulation
  • affinity
  • cations
  • sites

Cite this

Williams, L., Hannah, L. T., Hastings, M. H., & Maywood, E. S. (1995). Melatonin receptors in the rat brain and pituitary. Journal of Pineal Research, 19(4), 173-177.

Melatonin receptors in the rat brain and pituitary. / Williams, Lynda; Hannah, L T ; Hastings, M H ; Maywood, E S .

In: Journal of Pineal Research, Vol. 19, No. 4, 11.1995, p. 173-177.

Research output: Contribution to journalArticle

Williams, L, Hannah, LT, Hastings, MH & Maywood, ES 1995, 'Melatonin receptors in the rat brain and pituitary', Journal of Pineal Research, vol. 19, no. 4, pp. 173-177.
Williams L, Hannah LT, Hastings MH, Maywood ES. Melatonin receptors in the rat brain and pituitary. Journal of Pineal Research. 1995 Nov;19(4):173-177.
Williams, Lynda ; Hannah, L T ; Hastings, M H ; Maywood, E S . / Melatonin receptors in the rat brain and pituitary. In: Journal of Pineal Research. 1995 ; Vol. 19, No. 4. pp. 173-177.
@article{3b14ca683d8c48be9d65de36e09c79aa,
title = "Melatonin receptors in the rat brain and pituitary",
abstract = "2-(I-125)iodomelatonin binding has been mapped and characterized in the brain and pituitary of the male laboratory rat using quantitative in vitro autoradiography. Specific binding was defined as that completely displaced in the presence of 1 mu M melatonin. In the brain high levels of binding were localized over the suprachiasmatic nucleus (SCN), the area postrema (AP), and the spinal tract of the trigeminal nerve (Sp5). Lower densities of binding were found over the medial preoptic area (MPA), the septohypothalamic nuclei (SHy), the anterior hypothalamic area (AHA), the nuclei of the lateral olfactory tract (LOT), the paraventricular (PV), anteroventral (AV) and intermediodorsal (IMD) nuclei of the thalamus, the medial region of the lateral habenular (Lhb), the nuclei of the stria medullaris (SM), the basolateral (BL) and medial (ME) amygdaloid nuclei, the ventromedial nuclei (VMH), the arcuate nuclei (Are), the subiculum of the hippocampus (S) and the lateral mammillary nuclei (LM). High levels of binding were also present over the pars tuberalis of the pituitary (PT) and the anterior and posterior cerebral arteries (CA). In both neuronal and non-neuronal areas, specific binding was time dependent and partially reversible in the presence of 1 mu M melatonin. Binding was also saturable and of high affinity with dissociation constants (Kd) in the low picomolar range and was significantly inhibited in the presence of 10(-4)M guanosine 5'-0-(3-thiotriphosphate) (GTP gamma S) and 150 mM NaCl in all regions examined, indicating the presence of high affinity G-protein coupled melatonin receptors.",
keywords = "melatonin receptors, rat brain, pars tuberalis, G-protein, anterodorsal thalamic nuceli, guanine-nucleotides, invitro autoradiography, binding, localization, modulation, affinity, cations, sites",
author = "Lynda Williams and Hannah, {L T} and Hastings, {M H} and Maywood, {E S}",
year = "1995",
month = "11",
language = "English",
volume = "19",
pages = "173--177",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Melatonin receptors in the rat brain and pituitary

AU - Williams, Lynda

AU - Hannah, L T

AU - Hastings, M H

AU - Maywood, E S

PY - 1995/11

Y1 - 1995/11

N2 - 2-(I-125)iodomelatonin binding has been mapped and characterized in the brain and pituitary of the male laboratory rat using quantitative in vitro autoradiography. Specific binding was defined as that completely displaced in the presence of 1 mu M melatonin. In the brain high levels of binding were localized over the suprachiasmatic nucleus (SCN), the area postrema (AP), and the spinal tract of the trigeminal nerve (Sp5). Lower densities of binding were found over the medial preoptic area (MPA), the septohypothalamic nuclei (SHy), the anterior hypothalamic area (AHA), the nuclei of the lateral olfactory tract (LOT), the paraventricular (PV), anteroventral (AV) and intermediodorsal (IMD) nuclei of the thalamus, the medial region of the lateral habenular (Lhb), the nuclei of the stria medullaris (SM), the basolateral (BL) and medial (ME) amygdaloid nuclei, the ventromedial nuclei (VMH), the arcuate nuclei (Are), the subiculum of the hippocampus (S) and the lateral mammillary nuclei (LM). High levels of binding were also present over the pars tuberalis of the pituitary (PT) and the anterior and posterior cerebral arteries (CA). In both neuronal and non-neuronal areas, specific binding was time dependent and partially reversible in the presence of 1 mu M melatonin. Binding was also saturable and of high affinity with dissociation constants (Kd) in the low picomolar range and was significantly inhibited in the presence of 10(-4)M guanosine 5'-0-(3-thiotriphosphate) (GTP gamma S) and 150 mM NaCl in all regions examined, indicating the presence of high affinity G-protein coupled melatonin receptors.

AB - 2-(I-125)iodomelatonin binding has been mapped and characterized in the brain and pituitary of the male laboratory rat using quantitative in vitro autoradiography. Specific binding was defined as that completely displaced in the presence of 1 mu M melatonin. In the brain high levels of binding were localized over the suprachiasmatic nucleus (SCN), the area postrema (AP), and the spinal tract of the trigeminal nerve (Sp5). Lower densities of binding were found over the medial preoptic area (MPA), the septohypothalamic nuclei (SHy), the anterior hypothalamic area (AHA), the nuclei of the lateral olfactory tract (LOT), the paraventricular (PV), anteroventral (AV) and intermediodorsal (IMD) nuclei of the thalamus, the medial region of the lateral habenular (Lhb), the nuclei of the stria medullaris (SM), the basolateral (BL) and medial (ME) amygdaloid nuclei, the ventromedial nuclei (VMH), the arcuate nuclei (Are), the subiculum of the hippocampus (S) and the lateral mammillary nuclei (LM). High levels of binding were also present over the pars tuberalis of the pituitary (PT) and the anterior and posterior cerebral arteries (CA). In both neuronal and non-neuronal areas, specific binding was time dependent and partially reversible in the presence of 1 mu M melatonin. Binding was also saturable and of high affinity with dissociation constants (Kd) in the low picomolar range and was significantly inhibited in the presence of 10(-4)M guanosine 5'-0-(3-thiotriphosphate) (GTP gamma S) and 150 mM NaCl in all regions examined, indicating the presence of high affinity G-protein coupled melatonin receptors.

KW - melatonin receptors

KW - rat brain

KW - pars tuberalis

KW - G-protein

KW - anterodorsal thalamic nuceli

KW - guanine-nucleotides

KW - invitro autoradiography

KW - binding

KW - localization

KW - modulation

KW - affinity

KW - cations

KW - sites

M3 - Article

VL - 19

SP - 173

EP - 177

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 4

ER -