Membrane nanotubes in myeloid cells in the adult mouse cornea represent a novel mode of immune cell interaction

Yashar Seyed-Razavi, Michael Hickey, Lucia Kuffova, Paul McMenamin, Holly Chinnery

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Membrane nanotubes (MNTs) are newly discovered cellular extensions that are either blind-ended or can connect widely separated cells. They have predominantly been investigated in cultured isolated cells, however, previously we were the first group to demonstrate the existence of these structures in vivo in intact mammalian tissues. We previously demonstrated the frequency of both cell–cell or bridging MNTs and blind-ended MNTs was greatest between major histocompatibility complex (MHC) class II+ cells during corneal injury or TLR ligand-mediated inflammation. The present study aimed to further explore the dynamics of MNT formation and their size, presence in another tissue, the dura mater, and response to stress factors and an active local viral infection of the murine cornea. Confocal live cell imaging of myeloid-derived cells in inflamed corneal explants from Cx3cr1GFP and CD11ceYFP transgenic mice revealed that MNTs form de novo at a rate of 15.5¿µm/min. This observation contrasts with previous studies that demonstrated that in vitro these structures originate from cell–cell contacts. Conditions that promote formation of MNTs include inflammation in vivo and cell stress due to serum starvation ex vivo. Herpes simplex virus-1 infection did not cause a significant increase in MNT numbers in myeloid cells in the cornea above that observed in injury controls, confirming that corneal epithelium injury alone elicits MNT formation in vivo. These novel observations extend the currently limited understanding of MNTs in live mammalian tissues.
Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalImmunology and Cell Biology
Volume91
Issue number1
Early online date13 Nov 2012
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Nanotubes
Myeloid Cells
Cell Communication
Cornea
Membranes
Virus Diseases
Inflammation
Dura Mater
Corneal Epithelium
Human Herpesvirus 1
Starvation
Major Histocompatibility Complex
Transgenic Mice
Cultured Cells
Ligands

Keywords

  • CD11c
  • corneal inflammation
  • Cx3cr1
  • dendritic cells
  • intercellular communication
  • macrophages

Cite this

Membrane nanotubes in myeloid cells in the adult mouse cornea represent a novel mode of immune cell interaction. / Seyed-Razavi, Yashar; Hickey, Michael; Kuffova, Lucia; McMenamin, Paul; Chinnery, Holly.

In: Immunology and Cell Biology, Vol. 91, No. 1, 01.2013, p. 89-95.

Research output: Contribution to journalArticle

Seyed-Razavi, Yashar ; Hickey, Michael ; Kuffova, Lucia ; McMenamin, Paul ; Chinnery, Holly. / Membrane nanotubes in myeloid cells in the adult mouse cornea represent a novel mode of immune cell interaction. In: Immunology and Cell Biology. 2013 ; Vol. 91, No. 1. pp. 89-95.
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