Mesenchymal stem cells: a perspective from in vitro cultures to in vivo migration and niches

Andrea Augello, Tobias B Kurth, Cosimo De Bari

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Mesenchymal Stromal Progenitor/Stem Cells (MSCs) are a rare population of non-hematopoietic stromal cells, present in the bone marrow and most connective tissues of the body. They are capable of differentiation into mesenchymal tissues such as bone, cartilage, adipose tissue and muscle. In the absence of specific markers, MSCs have been defined following isolation and culture expansion, by their expression of various molecules including CD90, CD105 and CD73 and absence of markers like CD34, CD45, and CD14. MSCs have extensive proliferative ability in culture in an uncommitted state while retaining their multilineage differentiation potential, which make them attractive candidates for biological cell-based tissue repair approaches. However, their identity in their tissues of origin is not clear and the niches in which they reside are not defined. This review addresses the current state of MSC research including the differentiation potency of culture expanded MSCs, expression of chemokines and their receptors in MSCs--both relevant issues for the advocated use of MSCs for tissue repair and their systemic delivery to the affected tissues. It also reviews current knowledge of MSC niches in their native tissues, addressing the relationship with pericytes. Finally, it provides a scientific basis for the requirement of a thorough characterisation of the endogenous MSC niches within their native tissues in vivo. The knowledge of MSC niches will instruct development of innovative therapeutic measures such as producing pharmacological substances that target endogenous MSCs and their niches in order to activate and guide intrinsic repair and to improve disease outcomes.
Original languageEnglish
Pages (from-to)121-133
Number of pages13
JournalEuropean Cells & Materials
Volume20
Publication statusPublished - 1 Sep 2010

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Cell culture
Stem Cells
Stromal Cells
Tissue
Stem Cell Niche
Repair
Bone
In Vitro Techniques
Chemokine Receptors
Cartilage
Muscle
Stem Cell Research
Pericytes
Cells
Connective Tissue
Molecules
Adipose Tissue

Keywords

  • stem cells
  • mesenchymal stromal progenitors
  • niche
  • pericytes
  • regenerative medicine
  • migration
  • chemokines
  • differentiation
  • cartilage
  • bone

Cite this

Mesenchymal stem cells : a perspective from in vitro cultures to in vivo migration and niches. / Augello, Andrea; Kurth, Tobias B; De Bari, Cosimo.

In: European Cells & Materials, Vol. 20, 01.09.2010, p. 121-133.

Research output: Contribution to journalArticle

@article{19597bc70d5441f98775ab831be2ec4e,
title = "Mesenchymal stem cells: a perspective from in vitro cultures to in vivo migration and niches",
abstract = "Mesenchymal Stromal Progenitor/Stem Cells (MSCs) are a rare population of non-hematopoietic stromal cells, present in the bone marrow and most connective tissues of the body. They are capable of differentiation into mesenchymal tissues such as bone, cartilage, adipose tissue and muscle. In the absence of specific markers, MSCs have been defined following isolation and culture expansion, by their expression of various molecules including CD90, CD105 and CD73 and absence of markers like CD34, CD45, and CD14. MSCs have extensive proliferative ability in culture in an uncommitted state while retaining their multilineage differentiation potential, which make them attractive candidates for biological cell-based tissue repair approaches. However, their identity in their tissues of origin is not clear and the niches in which they reside are not defined. This review addresses the current state of MSC research including the differentiation potency of culture expanded MSCs, expression of chemokines and their receptors in MSCs--both relevant issues for the advocated use of MSCs for tissue repair and their systemic delivery to the affected tissues. It also reviews current knowledge of MSC niches in their native tissues, addressing the relationship with pericytes. Finally, it provides a scientific basis for the requirement of a thorough characterisation of the endogenous MSC niches within their native tissues in vivo. The knowledge of MSC niches will instruct development of innovative therapeutic measures such as producing pharmacological substances that target endogenous MSCs and their niches in order to activate and guide intrinsic repair and to improve disease outcomes.",
keywords = "stem cells, mesenchymal stromal progenitors, niche, pericytes, regenerative medicine, migration, chemokines, differentiation, cartilage, bone",
author = "Andrea Augello and Kurth, {Tobias B} and {De Bari}, Cosimo",
year = "2010",
month = "9",
day = "1",
language = "English",
volume = "20",
pages = "121--133",
journal = "European Cells & Materials",
issn = "1473-2262",
publisher = "Swiss Society for Biomaterials",

}

TY - JOUR

T1 - Mesenchymal stem cells

T2 - a perspective from in vitro cultures to in vivo migration and niches

AU - Augello, Andrea

AU - Kurth, Tobias B

AU - De Bari, Cosimo

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Mesenchymal Stromal Progenitor/Stem Cells (MSCs) are a rare population of non-hematopoietic stromal cells, present in the bone marrow and most connective tissues of the body. They are capable of differentiation into mesenchymal tissues such as bone, cartilage, adipose tissue and muscle. In the absence of specific markers, MSCs have been defined following isolation and culture expansion, by their expression of various molecules including CD90, CD105 and CD73 and absence of markers like CD34, CD45, and CD14. MSCs have extensive proliferative ability in culture in an uncommitted state while retaining their multilineage differentiation potential, which make them attractive candidates for biological cell-based tissue repair approaches. However, their identity in their tissues of origin is not clear and the niches in which they reside are not defined. This review addresses the current state of MSC research including the differentiation potency of culture expanded MSCs, expression of chemokines and their receptors in MSCs--both relevant issues for the advocated use of MSCs for tissue repair and their systemic delivery to the affected tissues. It also reviews current knowledge of MSC niches in their native tissues, addressing the relationship with pericytes. Finally, it provides a scientific basis for the requirement of a thorough characterisation of the endogenous MSC niches within their native tissues in vivo. The knowledge of MSC niches will instruct development of innovative therapeutic measures such as producing pharmacological substances that target endogenous MSCs and their niches in order to activate and guide intrinsic repair and to improve disease outcomes.

AB - Mesenchymal Stromal Progenitor/Stem Cells (MSCs) are a rare population of non-hematopoietic stromal cells, present in the bone marrow and most connective tissues of the body. They are capable of differentiation into mesenchymal tissues such as bone, cartilage, adipose tissue and muscle. In the absence of specific markers, MSCs have been defined following isolation and culture expansion, by their expression of various molecules including CD90, CD105 and CD73 and absence of markers like CD34, CD45, and CD14. MSCs have extensive proliferative ability in culture in an uncommitted state while retaining their multilineage differentiation potential, which make them attractive candidates for biological cell-based tissue repair approaches. However, their identity in their tissues of origin is not clear and the niches in which they reside are not defined. This review addresses the current state of MSC research including the differentiation potency of culture expanded MSCs, expression of chemokines and their receptors in MSCs--both relevant issues for the advocated use of MSCs for tissue repair and their systemic delivery to the affected tissues. It also reviews current knowledge of MSC niches in their native tissues, addressing the relationship with pericytes. Finally, it provides a scientific basis for the requirement of a thorough characterisation of the endogenous MSC niches within their native tissues in vivo. The knowledge of MSC niches will instruct development of innovative therapeutic measures such as producing pharmacological substances that target endogenous MSCs and their niches in order to activate and guide intrinsic repair and to improve disease outcomes.

KW - stem cells

KW - mesenchymal stromal progenitors

KW - niche

KW - pericytes

KW - regenerative medicine

KW - migration

KW - chemokines

KW - differentiation

KW - cartilage

KW - bone

M3 - Article

VL - 20

SP - 121

EP - 133

JO - European Cells & Materials

JF - European Cells & Materials

SN - 1473-2262

ER -