Metabolic analysis of the removal of formic acid by unacclimated activated sludge

C. Cruz Viggi, D. Dionisi, A. Miccheli, M. Valerio, M. Majone

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This paper investigates the removal of formicacid by unacclimated biomass from a municipal activatedsludge wastewater treatment plant. The biomass was initially able to remove formicacid, but its removal rate and Oxygen Uptake Rate (OUR) decreased with time, until formicacidremoval stopped before the formicacid had been exhausted. Formaldehyde was removed in a similar way, whereas the same biomass was simultaneously able to grow and store PHAs when acetic acid was used as substrate.

Batch tests with glycine and 13C NMR analysis were performed, showing that unacclimated biomass was not able to synthesize all the metabolic intermediates from formicacid alone. At least glycine needed to be externally supplemented, in order to activate the serine synthesis pathway. A small amount of formicacidremoval in the absence of growth was also possible through formaldehyde formation and its further conversion to formalin (1,2-formaldehyde dimer), whereas no PHAs were formed.
Original languageEnglish
Pages (from-to)3393-3400
Number of pages8
JournalWater research
Volume44
Issue number11
DOIs
Publication statusPublished - Jun 2010

Fingerprint

formic acid
Formic acid
Formaldehyde
activated sludge
Biomass
formaldehyde
biomass
Amino acids
Acetic acid
Wastewater treatment
Dimers
acetic acid
nuclear magnetic resonance
Nuclear magnetic resonance
substrate
oxygen
Oxygen
removal
analysis
Substrates

Keywords

  • activated sludge
  • formic acid
  • NMR
  • 13C
  • isotopomer analysis

Cite this

Metabolic analysis of the removal of formic acid by unacclimated activated sludge. / Cruz Viggi, C.; Dionisi, D.; Miccheli, A.; Valerio, M.; Majone, M.

In: Water research, Vol. 44, No. 11, 06.2010, p. 3393-3400.

Research output: Contribution to journalArticle

Cruz Viggi, C. ; Dionisi, D. ; Miccheli, A. ; Valerio, M. ; Majone, M. / Metabolic analysis of the removal of formic acid by unacclimated activated sludge. In: Water research. 2010 ; Vol. 44, No. 11. pp. 3393-3400.
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