Metabolic switches and adaptations deduced from the proteomes of streptomyces coelicolor wild type and phoP mutant grown in batch culture

Louise Thomas, David A. Hodgson, Alexander Wentzel, Kay Nieselt, Trond E. Ellingsen, Jonathan Moore, Edward R. Morrissey, Roxane Legaie, The STREAM Consortium, Wolfgang Wohlleben, Antonio Rodríguez-García, Juan F. Martín, Nigel J. Burroughs, Elizabeth M. H. Wellington, Margaret C. M. Smith

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Abstract

Bacteria in the genus Streptomyces are soil-dwelling oligotrophs and important producers of secondary metabolites. Previously, we showed that global messenger RNA expression was subject to a series of metabolic and regulatory switches during the lifetime of a fermentor batch culture of Streptomyces coelicolor M145. Here we analyze the proteome from eight time points from the same fermentor culture and, because phosphate availability is an important regulator of secondary metabolite production, compare this to the proteome of a similar time course from an S. coelicolor mutant, INB201 (¿phoP), defective in the control of phosphate utilization. The proteomes provide a detailed view of enzymes involved in central carbon and nitrogen metabolism. Trends in protein expression over the time courses were deduced from a protein abundance index, which also revealed the importance of stress pathway proteins in both cultures. As expected, the ¿phoP mutant was deficient in expression of PhoP-dependent genes, and several putatively compensatory metabolic and regulatory pathways for phosphate scavenging were detected. Notably there is a succession of switches that coordinately induce the production of enzymes for five different secondary metabolite biosynthesis pathways over the course of the batch cultures.
Original languageEnglish
Article numberM111.013797
Number of pages18
JournalMolecular and Cellular Proteomics
Volume11
Issue number2
Early online date6 Dec 2011
DOIs
Publication statusPublished - 1 Feb 2012

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Streptomyces coelicolor
Batch Cell Culture Techniques
Proteome
Metabolites
Phosphates
Switches
Bioreactors
Proteins
Biosynthesis
Scavenging
Streptomyces
Enzymes
Heat-Shock Proteins
Metabolic Networks and Pathways
Metabolism
Bacteria
Nitrogen
Soil
Carbon
Genes

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Metabolic switches and adaptations deduced from the proteomes of streptomyces coelicolor wild type and phoP mutant grown in batch culture. / Thomas, Louise; Hodgson, David A.; Wentzel, Alexander; Nieselt, Kay; Ellingsen, Trond E.; Moore, Jonathan; Morrissey, Edward R.; Legaie, Roxane; The STREAM Consortium; Wohlleben, Wolfgang; Rodríguez-García, Antonio; Martín, Juan F.; Burroughs, Nigel J.; Wellington, Elizabeth M. H.; Smith, Margaret C. M.

In: Molecular and Cellular Proteomics, Vol. 11, No. 2, M111.013797, 01.02.2012.

Research output: Contribution to journalArticle

Thomas, L, Hodgson, DA, Wentzel, A, Nieselt, K, Ellingsen, TE, Moore, J, Morrissey, ER, Legaie, R, The STREAM Consortium, Wohlleben, W, Rodríguez-García, A, Martín, JF, Burroughs, NJ, Wellington, EMH & Smith, MCM 2012, 'Metabolic switches and adaptations deduced from the proteomes of streptomyces coelicolor wild type and phoP mutant grown in batch culture', Molecular and Cellular Proteomics, vol. 11, no. 2, M111.013797. https://doi.org/10.1074/mcp.M111.013797
Thomas, Louise ; Hodgson, David A. ; Wentzel, Alexander ; Nieselt, Kay ; Ellingsen, Trond E. ; Moore, Jonathan ; Morrissey, Edward R. ; Legaie, Roxane ; The STREAM Consortium ; Wohlleben, Wolfgang ; Rodríguez-García, Antonio ; Martín, Juan F. ; Burroughs, Nigel J. ; Wellington, Elizabeth M. H. ; Smith, Margaret C. M. / Metabolic switches and adaptations deduced from the proteomes of streptomyces coelicolor wild type and phoP mutant grown in batch culture. In: Molecular and Cellular Proteomics. 2012 ; Vol. 11, No. 2.
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