Metabotropic glutamate receptor activation and intracellular cyclic ADP-ribose release Ca2+ from the same store in cultured DRG neurones

J Pollock, J H Crawford, J F Wootton, G R Seabrook, R H Scott

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The whole cell patch clamp technique has been used to record Ca2+-activated cation and chloride conductances evoked by release of Ca2+ from intracellular stores of cultured neonatal dorsal root ganglion neurones. The aim of this study was to investigate metabotropic glutamate receptor (mGluR) mechanisms and evaluate a possible role for cyclic ADP-ribose as an intracellular signalling molecule. Glutamate and the metabotropic glutamate receptor agonist (1S,3R)-ACPD-evoked transient depolarizations, Ca2+-activated inward currents and rises in intracellular Ca2+. The (1S,3R)-ACPD-activated currents were insensitive to InsP(3) signalling inhibitors, heparin and pentosan polysulphate. Intracellular application of ryanodine alone activated currents in this study and proved a difficult tool to use as a potential inhibitor of cyclic ADP-ribose-mediated responses. However, intracellular dantrolene did attenuate both (1S,3R)-ACPD and cyclic ADP-ribose responses. Intracellular photorelease of cGMP and cyclic ADP-ribose mimicked the responses to mGluR receptor activation. Intracellular application of nicotinamide and W7 inhibited the responses to photoreleased cGMP but did not prevent responses to mGluR activation. The cyclic ADP-ribose receptor antagonist 8-amino cyclic ADP-ribose attenuated responses to (1S,3R)-ACPD, cGMP and cyclic ADP-ribose, but some Ca2+-activated inward currents were still observed in the presence of this antagonist. In conclusion, mGluR receptor activation, cGMP and cyclic ADP-ribose release Ca2+ from intracellular stores. Some evidence suggests that pharmacologically related pathways are involved. (C) Harcourt Publishers Ltd 1999.

Original languageEnglish
Pages (from-to)139-148
Number of pages10
JournalCell Calcium
Volume26
Publication statusPublished - 1999

Keywords

  • ROOT GANGLION NEURONS
  • BULLFROG SYMPATHETIC NEURONS
  • SEA-URCHIN EGGS
  • SARCOPLASMIC-RETICULUM
  • RYANODINE RECEPTOR
  • SKELETAL-MUSCLE
  • CELLS
  • CURRENTS
  • CHANNELS
  • INHIBITION

Cite this

Pollock, J., Crawford, J. H., Wootton, J. F., Seabrook, G. R., & Scott, R. H. (1999). Metabotropic glutamate receptor activation and intracellular cyclic ADP-ribose release Ca2+ from the same store in cultured DRG neurones. Cell Calcium, 26, 139-148.

Metabotropic glutamate receptor activation and intracellular cyclic ADP-ribose release Ca2+ from the same store in cultured DRG neurones. / Pollock, J ; Crawford, J H ; Wootton, J F ; Seabrook, G R ; Scott, R H .

In: Cell Calcium, Vol. 26, 1999, p. 139-148.

Research output: Contribution to journalArticle

Pollock, J ; Crawford, J H ; Wootton, J F ; Seabrook, G R ; Scott, R H . / Metabotropic glutamate receptor activation and intracellular cyclic ADP-ribose release Ca2+ from the same store in cultured DRG neurones. In: Cell Calcium. 1999 ; Vol. 26. pp. 139-148.
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T1 - Metabotropic glutamate receptor activation and intracellular cyclic ADP-ribose release Ca2+ from the same store in cultured DRG neurones

AU - Pollock, J

AU - Crawford, J H

AU - Wootton, J F

AU - Seabrook, G R

AU - Scott, R H

PY - 1999

Y1 - 1999

N2 - The whole cell patch clamp technique has been used to record Ca2+-activated cation and chloride conductances evoked by release of Ca2+ from intracellular stores of cultured neonatal dorsal root ganglion neurones. The aim of this study was to investigate metabotropic glutamate receptor (mGluR) mechanisms and evaluate a possible role for cyclic ADP-ribose as an intracellular signalling molecule. Glutamate and the metabotropic glutamate receptor agonist (1S,3R)-ACPD-evoked transient depolarizations, Ca2+-activated inward currents and rises in intracellular Ca2+. The (1S,3R)-ACPD-activated currents were insensitive to InsP(3) signalling inhibitors, heparin and pentosan polysulphate. Intracellular application of ryanodine alone activated currents in this study and proved a difficult tool to use as a potential inhibitor of cyclic ADP-ribose-mediated responses. However, intracellular dantrolene did attenuate both (1S,3R)-ACPD and cyclic ADP-ribose responses. Intracellular photorelease of cGMP and cyclic ADP-ribose mimicked the responses to mGluR receptor activation. Intracellular application of nicotinamide and W7 inhibited the responses to photoreleased cGMP but did not prevent responses to mGluR activation. The cyclic ADP-ribose receptor antagonist 8-amino cyclic ADP-ribose attenuated responses to (1S,3R)-ACPD, cGMP and cyclic ADP-ribose, but some Ca2+-activated inward currents were still observed in the presence of this antagonist. In conclusion, mGluR receptor activation, cGMP and cyclic ADP-ribose release Ca2+ from intracellular stores. Some evidence suggests that pharmacologically related pathways are involved. (C) Harcourt Publishers Ltd 1999.

AB - The whole cell patch clamp technique has been used to record Ca2+-activated cation and chloride conductances evoked by release of Ca2+ from intracellular stores of cultured neonatal dorsal root ganglion neurones. The aim of this study was to investigate metabotropic glutamate receptor (mGluR) mechanisms and evaluate a possible role for cyclic ADP-ribose as an intracellular signalling molecule. Glutamate and the metabotropic glutamate receptor agonist (1S,3R)-ACPD-evoked transient depolarizations, Ca2+-activated inward currents and rises in intracellular Ca2+. The (1S,3R)-ACPD-activated currents were insensitive to InsP(3) signalling inhibitors, heparin and pentosan polysulphate. Intracellular application of ryanodine alone activated currents in this study and proved a difficult tool to use as a potential inhibitor of cyclic ADP-ribose-mediated responses. However, intracellular dantrolene did attenuate both (1S,3R)-ACPD and cyclic ADP-ribose responses. Intracellular photorelease of cGMP and cyclic ADP-ribose mimicked the responses to mGluR receptor activation. Intracellular application of nicotinamide and W7 inhibited the responses to photoreleased cGMP but did not prevent responses to mGluR activation. The cyclic ADP-ribose receptor antagonist 8-amino cyclic ADP-ribose attenuated responses to (1S,3R)-ACPD, cGMP and cyclic ADP-ribose, but some Ca2+-activated inward currents were still observed in the presence of this antagonist. In conclusion, mGluR receptor activation, cGMP and cyclic ADP-ribose release Ca2+ from intracellular stores. Some evidence suggests that pharmacologically related pathways are involved. (C) Harcourt Publishers Ltd 1999.

KW - ROOT GANGLION NEURONS

KW - BULLFROG SYMPATHETIC NEURONS

KW - SEA-URCHIN EGGS

KW - SARCOPLASMIC-RETICULUM

KW - RYANODINE RECEPTOR

KW - SKELETAL-MUSCLE

KW - CELLS

KW - CURRENTS

KW - CHANNELS

KW - INHIBITION

M3 - Article

VL - 26

SP - 139

EP - 148

JO - Cell Calcium

JF - Cell Calcium

SN - 0143-4160

ER -