Metallothionein gene expression in human adipose tissue from lean and obese subjects

M S Do, S Y Nam, S E Hong, K W Kim, J S Duncan, J H Beattie, P Trayhurn

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    Expression of the gene encoding metallothionein, a low molecular-weight cysteine-rich, stress-response and metal-binding protein was examined in human adipose tissue. The mRNA for MT-2A, a major metallothionein isoform in humans, was detected in subcutaneous fat using a specific antisense oligonucleotide probe. The level of MT-2A mRNA was significantly higher in a group of obese subjects than in a lean group, paralleling a similar increase in ob mRNA. A two-week period on a diet of 800 calories/day did not lead to any significant change in MT-2 mRNA levels. Separation of mature adipocytes from the cells of the stromal vascular fraction indicated that in human adipose tissue the metallothionein (MT-2A) gene is expressed both in adipocytes and in other cells of the tissue.

    Original languageEnglish
    Pages (from-to)348-351
    Number of pages4
    JournalHormone and Metabolic Research
    Volume34
    Issue number6
    DOIs
    Publication statusPublished - Jun 2002

    Keywords

    • adipocytes
    • diet
    • northern blotting
    • resistin
    • secreted proteins
    • primary culture
    • preadipocytes
    • endocrine
    • secretion
    • differentiation
    • family
    • organ
    • fat

    Cite this

    Do, M. S., Nam, S. Y., Hong, S. E., Kim, K. W., Duncan, J. S., Beattie, J. H., & Trayhurn, P. (2002). Metallothionein gene expression in human adipose tissue from lean and obese subjects. Hormone and Metabolic Research, 34(6), 348-351. https://doi.org/10.1055/s-2002-33254

    Metallothionein gene expression in human adipose tissue from lean and obese subjects. / Do, M S ; Nam, S Y ; Hong, S E ; Kim, K W ; Duncan, J S ; Beattie, J H ; Trayhurn, P .

    In: Hormone and Metabolic Research, Vol. 34, No. 6, 06.2002, p. 348-351.

    Research output: Contribution to journalArticle

    Do, MS, Nam, SY, Hong, SE, Kim, KW, Duncan, JS, Beattie, JH & Trayhurn, P 2002, 'Metallothionein gene expression in human adipose tissue from lean and obese subjects', Hormone and Metabolic Research, vol. 34, no. 6, pp. 348-351. https://doi.org/10.1055/s-2002-33254
    Do, M S ; Nam, S Y ; Hong, S E ; Kim, K W ; Duncan, J S ; Beattie, J H ; Trayhurn, P . / Metallothionein gene expression in human adipose tissue from lean and obese subjects. In: Hormone and Metabolic Research. 2002 ; Vol. 34, No. 6. pp. 348-351.
    @article{f9f702d59321435598aa694802d17323,
    title = "Metallothionein gene expression in human adipose tissue from lean and obese subjects",
    abstract = "Expression of the gene encoding metallothionein, a low molecular-weight cysteine-rich, stress-response and metal-binding protein was examined in human adipose tissue. The mRNA for MT-2A, a major metallothionein isoform in humans, was detected in subcutaneous fat using a specific antisense oligonucleotide probe. The level of MT-2A mRNA was significantly higher in a group of obese subjects than in a lean group, paralleling a similar increase in ob mRNA. A two-week period on a diet of 800 calories/day did not lead to any significant change in MT-2 mRNA levels. Separation of mature adipocytes from the cells of the stromal vascular fraction indicated that in human adipose tissue the metallothionein (MT-2A) gene is expressed both in adipocytes and in other cells of the tissue.",
    keywords = "adipocytes, diet, northern blotting, resistin, secreted proteins, primary culture, preadipocytes, endocrine, secretion, differentiation, family, organ, fat",
    author = "Do, {M S} and Nam, {S Y} and Hong, {S E} and Kim, {K W} and Duncan, {J S} and Beattie, {J H} and P Trayhurn",
    year = "2002",
    month = "6",
    doi = "10.1055/s-2002-33254",
    language = "English",
    volume = "34",
    pages = "348--351",
    journal = "Hormone and Metabolic Research",
    issn = "0018-5043",
    publisher = "Georg Thieme Verlag",
    number = "6",

    }

    TY - JOUR

    T1 - Metallothionein gene expression in human adipose tissue from lean and obese subjects

    AU - Do, M S

    AU - Nam, S Y

    AU - Hong, S E

    AU - Kim, K W

    AU - Duncan, J S

    AU - Beattie, J H

    AU - Trayhurn, P

    PY - 2002/6

    Y1 - 2002/6

    N2 - Expression of the gene encoding metallothionein, a low molecular-weight cysteine-rich, stress-response and metal-binding protein was examined in human adipose tissue. The mRNA for MT-2A, a major metallothionein isoform in humans, was detected in subcutaneous fat using a specific antisense oligonucleotide probe. The level of MT-2A mRNA was significantly higher in a group of obese subjects than in a lean group, paralleling a similar increase in ob mRNA. A two-week period on a diet of 800 calories/day did not lead to any significant change in MT-2 mRNA levels. Separation of mature adipocytes from the cells of the stromal vascular fraction indicated that in human adipose tissue the metallothionein (MT-2A) gene is expressed both in adipocytes and in other cells of the tissue.

    AB - Expression of the gene encoding metallothionein, a low molecular-weight cysteine-rich, stress-response and metal-binding protein was examined in human adipose tissue. The mRNA for MT-2A, a major metallothionein isoform in humans, was detected in subcutaneous fat using a specific antisense oligonucleotide probe. The level of MT-2A mRNA was significantly higher in a group of obese subjects than in a lean group, paralleling a similar increase in ob mRNA. A two-week period on a diet of 800 calories/day did not lead to any significant change in MT-2 mRNA levels. Separation of mature adipocytes from the cells of the stromal vascular fraction indicated that in human adipose tissue the metallothionein (MT-2A) gene is expressed both in adipocytes and in other cells of the tissue.

    KW - adipocytes

    KW - diet

    KW - northern blotting

    KW - resistin

    KW - secreted proteins

    KW - primary culture

    KW - preadipocytes

    KW - endocrine

    KW - secretion

    KW - differentiation

    KW - family

    KW - organ

    KW - fat

    U2 - 10.1055/s-2002-33254

    DO - 10.1055/s-2002-33254

    M3 - Article

    VL - 34

    SP - 348

    EP - 351

    JO - Hormone and Metabolic Research

    JF - Hormone and Metabolic Research

    SN - 0018-5043

    IS - 6

    ER -