Metallothionein gene expression in human adipose tissue from lean and obese subjects

M S Do, S Y Nam, S E Hong, K W Kim, J S Duncan, J H Beattie, P Trayhurn

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    Abstract

    Expression of the gene encoding metallothionein, a low molecular-weight cysteine-rich, stress-response and metal-binding protein was examined in human adipose tissue. The mRNA for MT-2A, a major metallothionein isoform in humans, was detected in subcutaneous fat using a specific antisense oligonucleotide probe. The level of MT-2A mRNA was significantly higher in a group of obese subjects than in a lean group, paralleling a similar increase in ob mRNA. A two-week period on a diet of 800 calories/day did not lead to any significant change in MT-2 mRNA levels. Separation of mature adipocytes from the cells of the stromal vascular fraction indicated that in human adipose tissue the metallothionein (MT-2A) gene is expressed both in adipocytes and in other cells of the tissue.

    Original languageEnglish
    Pages (from-to)348-351
    Number of pages4
    JournalHormone and Metabolic Research
    Volume34
    Issue number6
    DOIs
    Publication statusPublished - Jun 2002

    Keywords

    • adipocytes
    • diet
    • northern blotting
    • resistin
    • secreted proteins
    • primary culture
    • preadipocytes
    • endocrine
    • secretion
    • differentiation
    • family
    • organ
    • fat

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