Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.
Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.
Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.
|Number of pages||4|
|Journal||Letters in Applied Microbiology|
|Publication status||Published - Jun 2003|
- methane oxidation
- nitrate reduction
- sulphate reduction
- mixed rumen microorgansims
- methanogenic bacteria
- sulfate reduction
- ovine rumen