Methane oxidation and its coupled electron-sink reactions in ruminal fluid

H  Kajikawa; C  Valdes; K  Hillman; R J Wallace; C J  Newbold

doi:10.1046/j.1472-765X.2003.01317.x

Methane oxidation and its coupled electron-sink reactions in ruminal fluid

H Kajikawa, C Valdes, K Hillman, R J Wallace, C J Newbold

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.

Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.

Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

Original language	English
Pages (from-to)	354-357
Number of pages	4
Journal	Letters in Applied Microbiology
Volume	36
Issue number	6
DOIs	https://doi.org/10.1046/j.1472-765X.2003.01317.x
Publication status	Published - Jun 2003

Keywords

methane oxidation
nitrate reduction
oxygen
rumen
sulphate reduction
mixed rumen microorgansims
methanogenic bacteria
sulfate reduction
ovine rumen
sediments
samples

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Cite this

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title = "Methane oxidation and its coupled electron-sink reactions in ruminal fluid",

abstract = "Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.",

keywords = "methane oxidation, nitrate reduction, oxygen, rumen, sulphate reduction, mixed rumen microorgansims, methanogenic bacteria, sulfate reduction, ovine rumen, sediments, samples",

author = "H Kajikawa and C Valdes and K Hillman and Wallace, {R J} and Newbold, {C J}",

year = "2003",

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T1 - Methane oxidation and its coupled electron-sink reactions in ruminal fluid

AU - Kajikawa, H

AU - Valdes, C

AU - Hillman, K

AU - Wallace, R J

AU - Newbold, C J

PY - 2003/6

Y1 - 2003/6

N2 - Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

AB - Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

KW - methane oxidation

KW - nitrate reduction

KW - oxygen

KW - rumen

KW - sulphate reduction

KW - mixed rumen microorgansims

KW - methanogenic bacteria

KW - sulfate reduction

KW - ovine rumen

KW - sediments

KW - samples

U2 - 10.1046/j.1472-765X.2003.01317.x

DO - 10.1046/j.1472-765X.2003.01317.x

M3 - Article

SN - 0266-8254

VL - 36

SP - 354

EP - 357

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 6

ER -

Methane oxidation and its coupled electron-sink reactions in ruminal fluid

Abstract

Keywords

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