Methane oxidation and its coupled electron-sink reactions in ruminal fluid

H Kajikawa, C Valdes, K Hillman, R J Wallace, C J Newbold

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.

Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.

Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

Original languageEnglish
Pages (from-to)354-357
Number of pages4
JournalLetters in Applied Microbiology
Volume36
Issue number6
DOIs
Publication statusPublished - Jun 2003

Keywords

  • methane oxidation
  • nitrate reduction
  • oxygen
  • rumen
  • sulphate reduction
  • mixed rumen microorgansims
  • methanogenic bacteria
  • sulfate reduction
  • ovine rumen
  • sediments
  • samples

Cite this

Methane oxidation and its coupled electron-sink reactions in ruminal fluid. / Kajikawa, H ; Valdes, C ; Hillman, K ; Wallace, R J; Newbold, C J .

In: Letters in Applied Microbiology, Vol. 36, No. 6, 06.2003, p. 354-357.

Research output: Contribution to journalArticle

Kajikawa, H ; Valdes, C ; Hillman, K ; Wallace, R J ; Newbold, C J . / Methane oxidation and its coupled electron-sink reactions in ruminal fluid. In: Letters in Applied Microbiology. 2003 ; Vol. 36, No. 6. pp. 354-357.
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