Mice that are resistant to diet-induced weight loss have greater food anticipatory activity and altered melanocortin-3 receptor (MC3R) and Dopamine receptor 2 (D2) gene expression

Lobke Maria Vaanholt (Corresponding Author), Sharon Elizabeth Mitchell, Rachel Elizabeth Sinclair, John Roger Speakman

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Abstract

Diet-induced weight loss varies considerably between individuals, but the mechanisms driving these individual differences remain largely unknown. Here we investigated whether key neuropeptides involved in the regulation of energy balance or reward systems were differentially expressed in mice that were prone or resistant to caloric restriction (CR) induced weight loss. Mice (n = 30 males and n = 34 females) were fed 70% of their own baseline ad libitum intake for 25 days, after which their brains were collected and expression of various neuropeptides were investigated and compared between the 10 male and 10 female mice that showed the greatest (high weight loss, HWL) or lowest weight loss (LWL) (n = 40 in total). HWL mice showed a differential neuropeptide profile to LWL in both sexes, characterised by increased expression of neuropeptide Y (NPY), agouti-related peptide (AgRP), leptin receptor (ObRb), and melanocortin 3 receptor (MC3R) in the arcuate nucleus. No changes in the expression of fat mass and obesity related gene (FTO) or suppressor of cytokine signalling 3 (Socs3) were observed. Levels of dopamine D2 receptor were decreased in the nucleus accumbens in HWL compared to LWL mice. HWL mice showed a stronger increase in food anticipatory activity (FAA) in response to CR than LWL mice. These results indicate that the mice prone to diet-induced weight loss experienced greater hunger, potentially driving their elevated FAA.
Original languageEnglish
Pages (from-to)83-93
Number of pages11
JournalHormones and Behavior
Volume73
Early online date27 Jun 2015
DOIs
Publication statusPublished - Jul 2015

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Receptor, Melanocortin, Type 3
Reducing Diet
Dopamine Receptors
Weight Loss
Gene Expression
Food
Neuropeptides
Caloric Restriction
Leptin Receptors
Arcuate Nucleus of Hypothalamus
Hunger
Peptide Receptors
Neuropeptide Y
Nucleus Accumbens
Reward
Individuality
Dopamine
Obesity
Fats

Keywords

  • individual variability
  • caloric restriction
  • energy balance
  • hypothalamus
  • NPY
  • POMC
  • AgRP
  • FTO
  • ObRb
  • leptin

Cite this

@article{16ba6a2f64334434b4bf33884ea8a64d,
title = "Mice that are resistant to diet-induced weight loss have greater food anticipatory activity and altered melanocortin-3 receptor (MC3R) and Dopamine receptor 2 (D2) gene expression",
abstract = "Diet-induced weight loss varies considerably between individuals, but the mechanisms driving these individual differences remain largely unknown. Here we investigated whether key neuropeptides involved in the regulation of energy balance or reward systems were differentially expressed in mice that were prone or resistant to caloric restriction (CR) induced weight loss. Mice (n = 30 males and n = 34 females) were fed 70{\%} of their own baseline ad libitum intake for 25 days, after which their brains were collected and expression of various neuropeptides were investigated and compared between the 10 male and 10 female mice that showed the greatest (high weight loss, HWL) or lowest weight loss (LWL) (n = 40 in total). HWL mice showed a differential neuropeptide profile to LWL in both sexes, characterised by increased expression of neuropeptide Y (NPY), agouti-related peptide (AgRP), leptin receptor (ObRb), and melanocortin 3 receptor (MC3R) in the arcuate nucleus. No changes in the expression of fat mass and obesity related gene (FTO) or suppressor of cytokine signalling 3 (Socs3) were observed. Levels of dopamine D2 receptor were decreased in the nucleus accumbens in HWL compared to LWL mice. HWL mice showed a stronger increase in food anticipatory activity (FAA) in response to CR than LWL mice. These results indicate that the mice prone to diet-induced weight loss experienced greater hunger, potentially driving their elevated FAA.",
keywords = "individual variability, caloric restriction, energy balance , hypothalamus, NPY, POMC, AgRP, FTO, ObRb, leptin",
author = "Vaanholt, {Lobke Maria} and Mitchell, {Sharon Elizabeth} and Sinclair, {Rachel Elizabeth} and Speakman, {John Roger}",
note = "Thanks to all members of the Energetics Group for their invaluable help at various stages throughout the project. This work was conducted under the Home Office Project License 60/3706 and L.M.V. was funded by a Rubicon grant from the Dutch Scientific Organisation (NWO) (Grant code, 825.08.014).",
year = "2015",
month = "7",
doi = "10.1016/j.yhbeh.2015.06.006",
language = "English",
volume = "73",
pages = "83--93",
journal = "Hormones and Behavior",
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T1 - Mice that are resistant to diet-induced weight loss have greater food anticipatory activity and altered melanocortin-3 receptor (MC3R) and Dopamine receptor 2 (D2) gene expression

AU - Vaanholt, Lobke Maria

AU - Mitchell, Sharon Elizabeth

AU - Sinclair, Rachel Elizabeth

AU - Speakman, John Roger

N1 - Thanks to all members of the Energetics Group for their invaluable help at various stages throughout the project. This work was conducted under the Home Office Project License 60/3706 and L.M.V. was funded by a Rubicon grant from the Dutch Scientific Organisation (NWO) (Grant code, 825.08.014).

PY - 2015/7

Y1 - 2015/7

N2 - Diet-induced weight loss varies considerably between individuals, but the mechanisms driving these individual differences remain largely unknown. Here we investigated whether key neuropeptides involved in the regulation of energy balance or reward systems were differentially expressed in mice that were prone or resistant to caloric restriction (CR) induced weight loss. Mice (n = 30 males and n = 34 females) were fed 70% of their own baseline ad libitum intake for 25 days, after which their brains were collected and expression of various neuropeptides were investigated and compared between the 10 male and 10 female mice that showed the greatest (high weight loss, HWL) or lowest weight loss (LWL) (n = 40 in total). HWL mice showed a differential neuropeptide profile to LWL in both sexes, characterised by increased expression of neuropeptide Y (NPY), agouti-related peptide (AgRP), leptin receptor (ObRb), and melanocortin 3 receptor (MC3R) in the arcuate nucleus. No changes in the expression of fat mass and obesity related gene (FTO) or suppressor of cytokine signalling 3 (Socs3) were observed. Levels of dopamine D2 receptor were decreased in the nucleus accumbens in HWL compared to LWL mice. HWL mice showed a stronger increase in food anticipatory activity (FAA) in response to CR than LWL mice. These results indicate that the mice prone to diet-induced weight loss experienced greater hunger, potentially driving their elevated FAA.

AB - Diet-induced weight loss varies considerably between individuals, but the mechanisms driving these individual differences remain largely unknown. Here we investigated whether key neuropeptides involved in the regulation of energy balance or reward systems were differentially expressed in mice that were prone or resistant to caloric restriction (CR) induced weight loss. Mice (n = 30 males and n = 34 females) were fed 70% of their own baseline ad libitum intake for 25 days, after which their brains were collected and expression of various neuropeptides were investigated and compared between the 10 male and 10 female mice that showed the greatest (high weight loss, HWL) or lowest weight loss (LWL) (n = 40 in total). HWL mice showed a differential neuropeptide profile to LWL in both sexes, characterised by increased expression of neuropeptide Y (NPY), agouti-related peptide (AgRP), leptin receptor (ObRb), and melanocortin 3 receptor (MC3R) in the arcuate nucleus. No changes in the expression of fat mass and obesity related gene (FTO) or suppressor of cytokine signalling 3 (Socs3) were observed. Levels of dopamine D2 receptor were decreased in the nucleus accumbens in HWL compared to LWL mice. HWL mice showed a stronger increase in food anticipatory activity (FAA) in response to CR than LWL mice. These results indicate that the mice prone to diet-induced weight loss experienced greater hunger, potentially driving their elevated FAA.

KW - individual variability

KW - caloric restriction

KW - energy balance

KW - hypothalamus

KW - NPY

KW - POMC

KW - AgRP

KW - FTO

KW - ObRb

KW - leptin

U2 - 10.1016/j.yhbeh.2015.06.006

DO - 10.1016/j.yhbeh.2015.06.006

M3 - Article

VL - 73

SP - 83

EP - 93

JO - Hormones and Behavior

JF - Hormones and Behavior

SN - 0018-506X

ER -