Micro-environmental conditions modulate protein secretion and infectivity of the Trichinella spiralis L1 larva

Francisco Bolas-Fernandez, Maria Auxiliadora Dea-Ayuela, Bernadette Connolly, Mark W Robinson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

After digestion of infected meat the free L1 of Trichinella spp. penetrate the intestinal mucosa where they moult to the mature adult stage. We have used proteomics to identify changes in protein secretion during in vitro culture of free T. spiralis muscle larvae under different environmental conditions, and to correlate these changes with their infectivity in mice. Muscle larvae were cultured in different media (RPMI-1640, C-199 and HBSS) under conditions of anaerobiosis, microaerobiosis and in 5% CO2 at 37 degrees C. Following incubation the larval excretory/secretory proteins were analysed by two-dimensional gel electrophoresis and the larvae were used to orally infect naive CD1 mice. For all culture media tested, infectivity of the L1 was preserved following incubation in anaerobic conditions. In contrast, the infectivity of worms cultured in nutrient-rich media was almost completely abolished in both microaerobiosis and in the presence of 5% CO2. Some infectivity was retained in poor or reduced culture media. Comparative analysis of larval infectivity and protein secretion showed that loss of infectivity correlated with the appearance of non-tyvelosylated proteins that in turn may be related to the onset of moulting. (C) 2008 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)236-239
Number of pages4
JournalVeterinary Parasitology
Volume159
Issue number3-4
Early online date22 Oct 2008
DOIs
Publication statusPublished - 23 Feb 2009

Keywords

  • Trichinella
  • proteomics
  • mass spectrometry
  • moulting
  • infectivity
  • rapid expulsion
  • antibodies
  • activation
  • cystatins
  • antigens
  • parasite
  • cloning
  • Trichinella

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