Abstract
The kinetics of zinc absorption, metabolism and excretion is extensively studied by nutritionists. Stable isotopes of zinc can be used to identify body zinc compartments that have different turnover kinetics. Since the compartments might belong to physiological subsections of different organs, there is a need for microsampling analysis to determine isotope ratios of the trace element zinc in tissue samples. Here, we study the feasibility to use laser ablation coupled to quadrupole ICP-MS for the determination of zinc tracers given to rats at different time points with the aim to generate isotope ratio bioimages of heart tissue. A double tracer (Zn-70 and Zn-67) experiment with rats was designed to label the exchangeable zinc pool as well as the stable zinc pool. The isotope ratios determined by laser ablation ICP-MS were evaluated by additional measurements of tissue digests. Accumulated tracers which made up more than 0.1% of total zinc could be identified in the tissues of the treated rats. It was established that at least 50 measurements from the microsampling were necessary to distinguish between controls and a tracer treated rat resulting in reduced resolution of the bioimage. With the parameters used, features in the tissue thin sections of at least 250 mu m(2) in size are necessary to detect the incorporation of a tracer. When different time points have to be measured, higher precisions are required and therefore a larger area needs to be ablated (1 mm(2)). Using the bioimages and pool measurements from one physiological feature, it was possible to show that the aorta cell walls incorporate the zinc tracer at the different time points.
| Original language | English |
|---|---|
| Pages (from-to) | 287-297 |
| Number of pages | 11 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 402 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2012 |
Keywords
- LA-ICP-MS
- zinc
- bioimaging
- tracer
- rat
- accumulation
- isotope ratio
- mapping
- thin sections
- heart
- Liver
- muscle
- plasma-mass spectrometry
- brain
- ZN
- FE
- CU
- ZN-65
Cite this
Microanalytical isotope ratio measurements and elemental mapping using laser ablation ICP-MS for tissue thin sections : zinc tracer studies in rats. / Urgast, Dagmar S.; Ou, Ou; Gordon, Margaret J.; Raab, Andrea; Nixon, Graeme F.; Kwun, In-Sook; Beattie, John H.; Feldmann, Joerg.
In: Analytical and Bioanalytical Chemistry, Vol. 402, No. 1, 01.2012, p. 287-297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microanalytical isotope ratio measurements and elemental mapping using laser ablation ICP-MS for tissue thin sections
T2 - zinc tracer studies in rats
AU - Urgast, Dagmar S.
AU - Ou, Ou
AU - Gordon, Margaret J.
AU - Raab, Andrea
AU - Nixon, Graeme F.
AU - Kwun, In-Sook
AU - Beattie, John H.
AU - Feldmann, Joerg
PY - 2012/1
Y1 - 2012/1
N2 - The kinetics of zinc absorption, metabolism and excretion is extensively studied by nutritionists. Stable isotopes of zinc can be used to identify body zinc compartments that have different turnover kinetics. Since the compartments might belong to physiological subsections of different organs, there is a need for microsampling analysis to determine isotope ratios of the trace element zinc in tissue samples. Here, we study the feasibility to use laser ablation coupled to quadrupole ICP-MS for the determination of zinc tracers given to rats at different time points with the aim to generate isotope ratio bioimages of heart tissue. A double tracer (Zn-70 and Zn-67) experiment with rats was designed to label the exchangeable zinc pool as well as the stable zinc pool. The isotope ratios determined by laser ablation ICP-MS were evaluated by additional measurements of tissue digests. Accumulated tracers which made up more than 0.1% of total zinc could be identified in the tissues of the treated rats. It was established that at least 50 measurements from the microsampling were necessary to distinguish between controls and a tracer treated rat resulting in reduced resolution of the bioimage. With the parameters used, features in the tissue thin sections of at least 250 mu m(2) in size are necessary to detect the incorporation of a tracer. When different time points have to be measured, higher precisions are required and therefore a larger area needs to be ablated (1 mm(2)). Using the bioimages and pool measurements from one physiological feature, it was possible to show that the aorta cell walls incorporate the zinc tracer at the different time points.
AB - The kinetics of zinc absorption, metabolism and excretion is extensively studied by nutritionists. Stable isotopes of zinc can be used to identify body zinc compartments that have different turnover kinetics. Since the compartments might belong to physiological subsections of different organs, there is a need for microsampling analysis to determine isotope ratios of the trace element zinc in tissue samples. Here, we study the feasibility to use laser ablation coupled to quadrupole ICP-MS for the determination of zinc tracers given to rats at different time points with the aim to generate isotope ratio bioimages of heart tissue. A double tracer (Zn-70 and Zn-67) experiment with rats was designed to label the exchangeable zinc pool as well as the stable zinc pool. The isotope ratios determined by laser ablation ICP-MS were evaluated by additional measurements of tissue digests. Accumulated tracers which made up more than 0.1% of total zinc could be identified in the tissues of the treated rats. It was established that at least 50 measurements from the microsampling were necessary to distinguish between controls and a tracer treated rat resulting in reduced resolution of the bioimage. With the parameters used, features in the tissue thin sections of at least 250 mu m(2) in size are necessary to detect the incorporation of a tracer. When different time points have to be measured, higher precisions are required and therefore a larger area needs to be ablated (1 mm(2)). Using the bioimages and pool measurements from one physiological feature, it was possible to show that the aorta cell walls incorporate the zinc tracer at the different time points.
KW - LA-ICP-MS
KW - zinc
KW - bioimaging
KW - tracer
KW - rat
KW - accumulation
KW - isotope ratio
KW - mapping
KW - thin sections
KW - heart
KW - Liver
KW - muscle
KW - plasma-mass spectrometry
KW - brain
KW - ZN
KW - FE
KW - CU
KW - ZN-65
U2 - 10.1007/s00216-011-5461-x
DO - 10.1007/s00216-011-5461-x
M3 - Article
VL - 402
SP - 287
EP - 297
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 1
ER -