Microbiota that affect risk for shigellosis in children in low-income countries

Brianna Lindsay; Joe Oundo; M Anowar Hossain; Martin Antonio; Boubou Tamboura; Alan W Walker; Joseph N Paulson; Julian Parkhill; Richard Omore; Abu S G Faruque; Suman Kumar Das; Usman N Ikumapayi; Mitchell Adeyemi; Doh Sanogo; Debasish Saha; Samba Sow; Tamer H Farag; Dilruba Nasrin; Shan Li; Sandra Panchalingam; Myron M Levine; Karen Kotloff; Laurence S Magder; Laura Hungerford; Halvor Sommerfelt; Mihai Pop; James P Nataro; O Colin Stine

doi:10.3201/eid2101.140795

Microbiota that affect risk for shigellosis in children in low-income countries

Brianna Lindsay, Joe Oundo, M Anowar Hossain, Martin Antonio, Boubou Tamboura, Alan W Walker, Joseph N Paulson, Julian Parkhill, Richard Omore, Abu S G Faruque, Suman Kumar Das, Usman N Ikumapayi, Mitchell Adeyemi, Doh Sanogo, Debasish Saha, Samba Sow, Tamer H Farag, Dilruba Nasrin, Shan Li, Sandra PanchalingamMyron M Levine, Karen Kotloff, Laurence S Magder, Laura Hungerford, Halvor Sommerfelt, Mihai Pop, James P Nataro, O Colin Stine

The Rowett Institute of Nutrition and Health

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Abstract

Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.

Original language	English
Pages (from-to)	242-250
Number of pages	9
Journal	Emerging Infectious Diseases
Volume	21
Issue number	2
DOIs	https://doi.org/10.3201/eid2101.140795
Publication status	Published - Feb 2015

Bibliographical note

Acknowledgements
We thank Carol Tackett for her consultation on clinical terminology and readability.

B.L., J.P.N., and O.C.S. developed the hypotheses; M.M.L., K.K., and J.P.N. led the Global Enteric Multicenter Study; B.L., J.O., M.A.H., M.A., B.T., R.O., A.S.G.F., S.K.D., U.N.I., M.A., D. Sanogo, D. Saha, S.S., T.H.F., D.N., and S.P. collected data and developed and maintain databases; A.W.W., J.N.P., J.P., M.P., and O.C.S. developed and performed 16S rRNA gene sequencing, assembly, and taxonomic identification; S.L., B.L., and S.P. performed laboratory protocols; and B.L., L.S.M., L.H., and H.S. performed analyses.

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Microbiota_ShigellosisRisk_EID2015
Emerging Infectious Diseases is published by the Centers for Disease Control and Prevention, a U.S. Government agency. Therefore, all materials published in Emerging Infectious Diseases are in the public domain and can be used without permission. Proper citation, however, is required.
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Lindsay, B., Oundo, J., Hossain, M. A., Antonio, M., Tamboura, B., Walker, A. W., Paulson, J. N., Parkhill, J., Omore, R., Faruque, A. S. G., Das, S. K., Ikumapayi, U. N., Adeyemi, M., Sanogo, D., Saha, D., Sow, S., Farag, T. H., Nasrin, D., Li, S., ... Stine, O. C. (2015). Microbiota that affect risk for shigellosis in children in low-income countries. Emerging Infectious Diseases, 21(2), 242-250. https://doi.org/10.3201/eid2101.140795

Lindsay, B, Oundo, J, Hossain, MA, Antonio, M, Tamboura, B, Walker, AW, Paulson, JN, Parkhill, J, Omore, R, Faruque, ASG, Das, SK, Ikumapayi, UN, Adeyemi, M, Sanogo, D, Saha, D, Sow, S, Farag, TH, Nasrin, D, Li, S, Panchalingam, S, Levine, MM, Kotloff, K, Magder, LS, Hungerford, L, Sommerfelt, H, Pop, M, Nataro, JP & Stine, OC 2015, 'Microbiota that affect risk for shigellosis in children in low-income countries', Emerging Infectious Diseases, vol. 21, no. 2, pp. 242-250. https://doi.org/10.3201/eid2101.140795

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title = "Microbiota that affect risk for shigellosis in children in low-income countries",

abstract = "Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.",

author = "Brianna Lindsay and Joe Oundo and Hossain, {M Anowar} and Martin Antonio and Boubou Tamboura and Walker, {Alan W} and Paulson, {Joseph N} and Julian Parkhill and Richard Omore and Faruque, {Abu S G} and Das, {Suman Kumar} and Ikumapayi, {Usman N} and Mitchell Adeyemi and Doh Sanogo and Debasish Saha and Samba Sow and Farag, {Tamer H} and Dilruba Nasrin and Shan Li and Sandra Panchalingam and Levine, {Myron M} and Karen Kotloff and Magder, {Laurence S} and Laura Hungerford and Halvor Sommerfelt and Mihai Pop and Nataro, {James P} and Stine, {O Colin}",

note = "Acknowledgements We thank Carol Tackett for her consultation on clinical terminology and readability. B.L., J.P.N., and O.C.S. developed the hypotheses; M.M.L., K.K., and J.P.N. led the Global Enteric Multicenter Study; B.L., J.O., M.A.H., M.A., B.T., R.O., A.S.G.F., S.K.D., U.N.I., M.A., D. Sanogo, D. Saha, S.S., T.H.F., D.N., and S.P. collected data and developed and maintain databases; A.W.W., J.N.P., J.P., M.P., and O.C.S. developed and performed 16S rRNA gene sequencing, assembly, and taxonomic identification; S.L., B.L., and S.P. performed laboratory protocols; and B.L., L.S.M., L.H., and H.S. performed analyses. ",

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AU - Lindsay, Brianna

AU - Oundo, Joe

AU - Hossain, M Anowar

AU - Antonio, Martin

AU - Tamboura, Boubou

AU - Walker, Alan W

AU - Paulson, Joseph N

AU - Parkhill, Julian

AU - Omore, Richard

AU - Faruque, Abu S G

AU - Das, Suman Kumar

AU - Ikumapayi, Usman N

AU - Adeyemi, Mitchell

AU - Sanogo, Doh

AU - Saha, Debasish

AU - Sow, Samba

AU - Farag, Tamer H

AU - Nasrin, Dilruba

AU - Li, Shan

AU - Panchalingam, Sandra

AU - Levine, Myron M

AU - Kotloff, Karen

AU - Magder, Laurence S

AU - Hungerford, Laura

AU - Sommerfelt, Halvor

AU - Pop, Mihai

AU - Nataro, James P

AU - Stine, O Colin

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N2 - Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.

AB - Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.

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DO - 10.3201/eid2101.140795

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SN - 1080-6040

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JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 2

ER -

Microbiota that affect risk for shigellosis in children in low-income countries

Abstract

Bibliographical note

UN SDGs

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