Abstract
Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.
| Original language | English |
|---|---|
| Pages (from-to) | 242-250 |
| Number of pages | 9 |
| Journal | Emerging Infectious Diseases |
| Volume | 21 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2015 |
Fingerprint
Cite this
Microbiota that affect risk for shigellosis in children in low-income countries. / Lindsay, Brianna; Oundo, Joe; Hossain, M Anowar; Antonio, Martin; Tamboura, Boubou; Walker, Alan W; Paulson, Joseph N; Parkhill, Julian; Omore, Richard; Faruque, Abu S G; Das, Suman Kumar; Ikumapayi, Usman N; Adeyemi, Mitchell; Sanogo, Doh; Saha, Debasish; Sow, Samba; Farag, Tamer H; Nasrin, Dilruba; Li, Shan; Panchalingam, Sandra; Levine, Myron M; Kotloff, Karen; Magder, Laurence S; Hungerford, Laura; Sommerfelt, Halvor; Pop, Mihai; Nataro, James P; Stine, O Colin.
In: Emerging Infectious Diseases, Vol. 21, No. 2, 02.2015, p. 242-250.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microbiota that affect risk for shigellosis in children in low-income countries
AU - Lindsay, Brianna
AU - Oundo, Joe
AU - Hossain, M Anowar
AU - Antonio, Martin
AU - Tamboura, Boubou
AU - Walker, Alan W
AU - Paulson, Joseph N
AU - Parkhill, Julian
AU - Omore, Richard
AU - Faruque, Abu S G
AU - Das, Suman Kumar
AU - Ikumapayi, Usman N
AU - Adeyemi, Mitchell
AU - Sanogo, Doh
AU - Saha, Debasish
AU - Sow, Samba
AU - Farag, Tamer H
AU - Nasrin, Dilruba
AU - Li, Shan
AU - Panchalingam, Sandra
AU - Levine, Myron M
AU - Kotloff, Karen
AU - Magder, Laurence S
AU - Hungerford, Laura
AU - Sommerfelt, Halvor
AU - Pop, Mihai
AU - Nataro, James P
AU - Stine, O Colin
N1 - Acknowledgements We thank Carol Tackett for her consultation on clinical terminology and readability. B.L., J.P.N., and O.C.S. developed the hypotheses; M.M.L., K.K., and J.P.N. led the Global Enteric Multicenter Study; B.L., J.O., M.A.H., M.A., B.T., R.O., A.S.G.F., S.K.D., U.N.I., M.A., D. Sanogo, D. Saha, S.S., T.H.F., D.N., and S.P. collected data and developed and maintain databases; A.W.W., J.N.P., J.P., M.P., and O.C.S. developed and performed 16S rRNA gene sequencing, assembly, and taxonomic identification; S.L., B.L., and S.P. performed laboratory protocols; and B.L., L.S.M., L.H., and H.S. performed analyses.
PY - 2015/2
Y1 - 2015/2
N2 - Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.
AB - Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.
U2 - 10.3201/eid2101.140795
DO - 10.3201/eid2101.140795
M3 - Article
C2 - 25625766
VL - 21
SP - 242
EP - 250
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
SN - 1080-6040
IS - 2
ER -