Micropropagation of Cupressus sempervirens L. and Chamaecyparis lawsoniana (A. MURR.) PAR.

K A Spanos, Andrew Pirrie, Stephen Woodward

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Shoots from 18-month-old seedlings of Cupressus sempervirens and Chamaecyparis lawsoniana were established in vitro on modified MURASHIGE and SKOOG medium. Proliferation of axillary shoots occurred without addition of benzyladenine, although a significant increase in numbers of shoots resulted on addition of 0.001 mg.l(-1) to 1.0 mg.l(-1) benzyladenine. Following conditioning on a growth regulator-free medium for 28 days, 95% of C. sempervirens shoots rooted on 1/2 strength medium containing 1% sucrose and 0.5 mg.l(-1) indole butyric acid. Similar levels of rooting were recorded with Chamaecyparis lawsoniana on the same medium containing 1 mg.l(-1) indole butyric acid. C. sempervirens shoots were also rooted in non-sterile conditions. Over 80% of rooted shoots of C. sempervirens were weaned into glasshouse conditions. The significance of these results in relation to propagation of cypress clones resistant to Seiridium canker is discussed.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalSilvae Genetica
Volume46
Issue number5
Publication statusPublished - 1997

Keywords

  • Cupressus sempervirens
  • Chamaecyparis lawsoniana
  • micropropagation
  • Seiridium cardinale
  • CULTURE
  • SPRUCE

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