Abstract
Shoots from 18-month-old seedlings of Cupressus sempervirens and Chamaecyparis lawsoniana were established in vitro on modified MURASHIGE and SKOOG medium. Proliferation of axillary shoots occurred without addition of benzyladenine, although a significant increase in numbers of shoots resulted on addition of 0.001 mg.l(-1) to 1.0 mg.l(-1) benzyladenine. Following conditioning on a growth regulator-free medium for 28 days, 95% of C. sempervirens shoots rooted on 1/2 strength medium containing 1% sucrose and 0.5 mg.l(-1) indole butyric acid. Similar levels of rooting were recorded with Chamaecyparis lawsoniana on the same medium containing 1 mg.l(-1) indole butyric acid. C. sempervirens shoots were also rooted in non-sterile conditions. Over 80% of rooted shoots of C. sempervirens were weaned into glasshouse conditions. The significance of these results in relation to propagation of cypress clones resistant to Seiridium canker is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 291-295 |
| Number of pages | 5 |
| Journal | Silvae Genetica |
| Volume | 46 |
| Issue number | 5 |
| Publication status | Published - 1997 |
Keywords
- Cupressus sempervirens
- Chamaecyparis lawsoniana
- micropropagation
- Seiridium cardinale
- CULTURE
- SPRUCE