Microsatellite DNA polymorphism confirms reproductive isolation and reveals differences in population genetic structure of cryptic pipistrelle bat species

Paul Adrian Racey, Elizabeth M. Barratt, Tamsin M. Burland, Robert Deaville, Dada Gotelli, Gareth Jones, Stuart B. Piertney

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Previous studies have indicated that the common European pipistrelle bat (Pipistrellus pipistrellus) comprises two cryptic species, P pipistrellus and Pipistrellus pygmaeus, which differ in echolocation call frequency and mitochondrial DNA sequence. However, levels of divergence based on nuclear markers have not been examined, and hence the potential for male-mediated gene flow between the species cannot be discounted. Moreover, little is known about population structure and migration patterns in either species. Here, we describe the use of microsatellites to investigate nuclear DNA differentiation between, and the pattern of population genetic structure within, the two cryptic pipistrelle species. In total, 1300 individuals from 82 maternity colonies were sampled across the British Isles and Continental Europe. We show, using multivariate analyses, that colonies of the same species are generally genetically more similar to each other than to those from the other species regardless of geographical location. Our findings support the hypothesis that the species are reproductively isolated. Significant patterns of genetic isolation by distance were identified in both species, indicating that mating may occur before any long-distance autumnal migration. The presence of a sea channel does not confer higher levels of genetic differentiation among colonies over and above distance alone in either species. Differences in genetic population structure were identified between the species, with R pipistrellus showing a wider range of levels of genetic differentiation among colonies and a stronger relationship between genetic and geographical distance than R pygmaeus. Differences in dispersal, mating behaviour, colony size and/or postglacial colonization patterns could contribute to the differences observed. (c) 2007 The Linnean Society of London.

Original languageEnglish
Pages (from-to)539-550
Number of pages12
JournalBiological Journal of the Linnean Society
Volume90
Issue number3
DOIs
Publication statusPublished - Mar 2007

Keywords

  • Chiroptera
  • isolation by distance
  • microsatellite
  • migration
  • 2 phonic types
  • sex-biased dispersal
  • myotis myotis
  • social-calls
  • chiroptera
  • vespertilionidae
  • morphology
  • mitochondrial
  • echolocation
  • regression

Cite this

Microsatellite DNA polymorphism confirms reproductive isolation and reveals differences in population genetic structure of cryptic pipistrelle bat species. / Racey, Paul Adrian; Barratt, Elizabeth M.; Burland, Tamsin M.; Deaville, Robert; Gotelli, Dada; Jones, Gareth; Piertney, Stuart B.

In: Biological Journal of the Linnean Society, Vol. 90, No. 3, 03.2007, p. 539-550.

Research output: Contribution to journalArticle

Racey, Paul Adrian ; Barratt, Elizabeth M. ; Burland, Tamsin M. ; Deaville, Robert ; Gotelli, Dada ; Jones, Gareth ; Piertney, Stuart B. / Microsatellite DNA polymorphism confirms reproductive isolation and reveals differences in population genetic structure of cryptic pipistrelle bat species. In: Biological Journal of the Linnean Society. 2007 ; Vol. 90, No. 3. pp. 539-550.
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abstract = "Previous studies have indicated that the common European pipistrelle bat (Pipistrellus pipistrellus) comprises two cryptic species, P pipistrellus and Pipistrellus pygmaeus, which differ in echolocation call frequency and mitochondrial DNA sequence. However, levels of divergence based on nuclear markers have not been examined, and hence the potential for male-mediated gene flow between the species cannot be discounted. Moreover, little is known about population structure and migration patterns in either species. Here, we describe the use of microsatellites to investigate nuclear DNA differentiation between, and the pattern of population genetic structure within, the two cryptic pipistrelle species. In total, 1300 individuals from 82 maternity colonies were sampled across the British Isles and Continental Europe. We show, using multivariate analyses, that colonies of the same species are generally genetically more similar to each other than to those from the other species regardless of geographical location. Our findings support the hypothesis that the species are reproductively isolated. Significant patterns of genetic isolation by distance were identified in both species, indicating that mating may occur before any long-distance autumnal migration. The presence of a sea channel does not confer higher levels of genetic differentiation among colonies over and above distance alone in either species. Differences in genetic population structure were identified between the species, with R pipistrellus showing a wider range of levels of genetic differentiation among colonies and a stronger relationship between genetic and geographical distance than R pygmaeus. Differences in dispersal, mating behaviour, colony size and/or postglacial colonization patterns could contribute to the differences observed. (c) 2007 The Linnean Society of London.",
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T1 - Microsatellite DNA polymorphism confirms reproductive isolation and reveals differences in population genetic structure of cryptic pipistrelle bat species

AU - Racey, Paul Adrian

AU - Barratt, Elizabeth M.

AU - Burland, Tamsin M.

AU - Deaville, Robert

AU - Gotelli, Dada

AU - Jones, Gareth

AU - Piertney, Stuart B.

PY - 2007/3

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N2 - Previous studies have indicated that the common European pipistrelle bat (Pipistrellus pipistrellus) comprises two cryptic species, P pipistrellus and Pipistrellus pygmaeus, which differ in echolocation call frequency and mitochondrial DNA sequence. However, levels of divergence based on nuclear markers have not been examined, and hence the potential for male-mediated gene flow between the species cannot be discounted. Moreover, little is known about population structure and migration patterns in either species. Here, we describe the use of microsatellites to investigate nuclear DNA differentiation between, and the pattern of population genetic structure within, the two cryptic pipistrelle species. In total, 1300 individuals from 82 maternity colonies were sampled across the British Isles and Continental Europe. We show, using multivariate analyses, that colonies of the same species are generally genetically more similar to each other than to those from the other species regardless of geographical location. Our findings support the hypothesis that the species are reproductively isolated. Significant patterns of genetic isolation by distance were identified in both species, indicating that mating may occur before any long-distance autumnal migration. The presence of a sea channel does not confer higher levels of genetic differentiation among colonies over and above distance alone in either species. Differences in genetic population structure were identified between the species, with R pipistrellus showing a wider range of levels of genetic differentiation among colonies and a stronger relationship between genetic and geographical distance than R pygmaeus. Differences in dispersal, mating behaviour, colony size and/or postglacial colonization patterns could contribute to the differences observed. (c) 2007 The Linnean Society of London.

AB - Previous studies have indicated that the common European pipistrelle bat (Pipistrellus pipistrellus) comprises two cryptic species, P pipistrellus and Pipistrellus pygmaeus, which differ in echolocation call frequency and mitochondrial DNA sequence. However, levels of divergence based on nuclear markers have not been examined, and hence the potential for male-mediated gene flow between the species cannot be discounted. Moreover, little is known about population structure and migration patterns in either species. Here, we describe the use of microsatellites to investigate nuclear DNA differentiation between, and the pattern of population genetic structure within, the two cryptic pipistrelle species. In total, 1300 individuals from 82 maternity colonies were sampled across the British Isles and Continental Europe. We show, using multivariate analyses, that colonies of the same species are generally genetically more similar to each other than to those from the other species regardless of geographical location. Our findings support the hypothesis that the species are reproductively isolated. Significant patterns of genetic isolation by distance were identified in both species, indicating that mating may occur before any long-distance autumnal migration. The presence of a sea channel does not confer higher levels of genetic differentiation among colonies over and above distance alone in either species. Differences in genetic population structure were identified between the species, with R pipistrellus showing a wider range of levels of genetic differentiation among colonies and a stronger relationship between genetic and geographical distance than R pygmaeus. Differences in dispersal, mating behaviour, colony size and/or postglacial colonization patterns could contribute to the differences observed. (c) 2007 The Linnean Society of London.

KW - Chiroptera

KW - isolation by distance

KW - microsatellite

KW - migration

KW - 2 phonic types

KW - sex-biased dispersal

KW - myotis myotis

KW - social-calls

KW - chiroptera

KW - vespertilionidae

KW - morphology

KW - mitochondrial

KW - echolocation

KW - regression

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DO - 10.1111/j.1095-8312.2007.00746.x

M3 - Article

VL - 90

SP - 539

EP - 550

JO - Biological Journal of the Linnean Society

JF - Biological Journal of the Linnean Society

SN - 0024-4066

IS - 3

ER -