Mineralisation of 14C 2,4-dichlorophenol and 14C glucose placed into the same or different hydrological domains as a bacterial inoculant

L. J. Shaw, Y. Beaton, S. Sousa, Lesley Anne Glover, Kenneth Stuart Killham, Andrew Alexander Meharg

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The spatial location of microorganisms in the soil three-dimensional structure with respect to their substrates plays an important role in the persistence and turnover of natural and xenobiotic organic compounds. To study the effect of spatial location oil the mineralisation of C-14-2,4-dichlorophenol (2,4-DCP, 0.15 or 0.31 mumol g(-1)) and C-14-glucose (2.77 mumol g(-1)), columns packed with autoclaved soil aggregates (2-5 mm) were used. Using a chloride tracer of water movement, the existence of 'immobile' water, which was by-passed by preferentially flowing 'mobile' water, was demonstrated. By manipulation of the soil Moisture Content, the substrates were putatively placed to these conceptual hydrological domains (immobile and mobile water). Leaching studies revealed that approximately 1.7 (glucose) and 3.4 (2,4-DCP) times the amount of substrate placed in mobile water was recovered in the first 4 fractions of leachate when compared to substrate placed in immobile water. The marked difference in the breakthrough curves was taken as evidence of Successful substrate placement. The 2,4-DCP degrading bacterium, Burkholderia sp. RASCc2, was inoculated in mobile water (1.8-5.2 x 10(7) cells g(-1) soil) and parameters (asymptote, time at maximum rate, calculated maximum rate) describing the mineralisation kinetics of 2,4-DCP and glucose previously added to immobile or mobile water domains were compared. For glucose, there was no significant effect (P > 0.1) of substrate placement Oil any of the mineralisation parameters. However, substrate placement had a significant effect (P < 0.05) on parameters describing 2.4-DCP mineralisation. In particular, 2,4-DCP added in mobile water was mineralised with a greater maximum rate and with a reduced time at maximum rate when compared to 2,4-DCP added to immobile water. The difference in response between the two test substrates may reflect the importance of sorption in controlling the spatial bioavailability of compounds in soil. (C) 2002 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)531-539
Number of pages8
JournalSoil Biology and Biochemistry
Volume34
DOIs
Publication statusPublished - 2002

Keywords

  • bioavailability
  • sequestration
  • spatial
  • inoculation
  • biodegradation
  • LEGUMINOSARUM BIOVAR TRIFOLII
  • HABITABLE PORE-SPACE
  • ORGANIC-COMPOUNDS
  • SOLUTE TRANSPORT
  • SOIL
  • SORPTION
  • BIODEGRADATION
  • BIOAVAILABILITY
  • SEQUESTRATION
  • PHENANTHRENE

Cite this

Mineralisation of 14C 2,4-dichlorophenol and 14C glucose placed into the same or different hydrological domains as a bacterial inoculant. / Shaw, L. J.; Beaton, Y.; Sousa, S.; Glover, Lesley Anne; Killham, Kenneth Stuart; Meharg, Andrew Alexander.

In: Soil Biology and Biochemistry, Vol. 34, 2002, p. 531-539.

Research output: Contribution to journalArticle

Shaw, L. J. ; Beaton, Y. ; Sousa, S. ; Glover, Lesley Anne ; Killham, Kenneth Stuart ; Meharg, Andrew Alexander. / Mineralisation of 14C 2,4-dichlorophenol and 14C glucose placed into the same or different hydrological domains as a bacterial inoculant. In: Soil Biology and Biochemistry. 2002 ; Vol. 34. pp. 531-539.
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abstract = "The spatial location of microorganisms in the soil three-dimensional structure with respect to their substrates plays an important role in the persistence and turnover of natural and xenobiotic organic compounds. To study the effect of spatial location oil the mineralisation of C-14-2,4-dichlorophenol (2,4-DCP, 0.15 or 0.31 mumol g(-1)) and C-14-glucose (2.77 mumol g(-1)), columns packed with autoclaved soil aggregates (2-5 mm) were used. Using a chloride tracer of water movement, the existence of 'immobile' water, which was by-passed by preferentially flowing 'mobile' water, was demonstrated. By manipulation of the soil Moisture Content, the substrates were putatively placed to these conceptual hydrological domains (immobile and mobile water). Leaching studies revealed that approximately 1.7 (glucose) and 3.4 (2,4-DCP) times the amount of substrate placed in mobile water was recovered in the first 4 fractions of leachate when compared to substrate placed in immobile water. The marked difference in the breakthrough curves was taken as evidence of Successful substrate placement. The 2,4-DCP degrading bacterium, Burkholderia sp. RASCc2, was inoculated in mobile water (1.8-5.2 x 10(7) cells g(-1) soil) and parameters (asymptote, time at maximum rate, calculated maximum rate) describing the mineralisation kinetics of 2,4-DCP and glucose previously added to immobile or mobile water domains were compared. For glucose, there was no significant effect (P > 0.1) of substrate placement Oil any of the mineralisation parameters. However, substrate placement had a significant effect (P < 0.05) on parameters describing 2.4-DCP mineralisation. In particular, 2,4-DCP added in mobile water was mineralised with a greater maximum rate and with a reduced time at maximum rate when compared to 2,4-DCP added to immobile water. The difference in response between the two test substrates may reflect the importance of sorption in controlling the spatial bioavailability of compounds in soil. (C) 2002 Elsevier Science Ltd. All rights reserved.",
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AU - Sousa, S.

AU - Glover, Lesley Anne

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AU - Meharg, Andrew Alexander

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N2 - The spatial location of microorganisms in the soil three-dimensional structure with respect to their substrates plays an important role in the persistence and turnover of natural and xenobiotic organic compounds. To study the effect of spatial location oil the mineralisation of C-14-2,4-dichlorophenol (2,4-DCP, 0.15 or 0.31 mumol g(-1)) and C-14-glucose (2.77 mumol g(-1)), columns packed with autoclaved soil aggregates (2-5 mm) were used. Using a chloride tracer of water movement, the existence of 'immobile' water, which was by-passed by preferentially flowing 'mobile' water, was demonstrated. By manipulation of the soil Moisture Content, the substrates were putatively placed to these conceptual hydrological domains (immobile and mobile water). Leaching studies revealed that approximately 1.7 (glucose) and 3.4 (2,4-DCP) times the amount of substrate placed in mobile water was recovered in the first 4 fractions of leachate when compared to substrate placed in immobile water. The marked difference in the breakthrough curves was taken as evidence of Successful substrate placement. The 2,4-DCP degrading bacterium, Burkholderia sp. RASCc2, was inoculated in mobile water (1.8-5.2 x 10(7) cells g(-1) soil) and parameters (asymptote, time at maximum rate, calculated maximum rate) describing the mineralisation kinetics of 2,4-DCP and glucose previously added to immobile or mobile water domains were compared. For glucose, there was no significant effect (P > 0.1) of substrate placement Oil any of the mineralisation parameters. However, substrate placement had a significant effect (P < 0.05) on parameters describing 2.4-DCP mineralisation. In particular, 2,4-DCP added in mobile water was mineralised with a greater maximum rate and with a reduced time at maximum rate when compared to 2,4-DCP added to immobile water. The difference in response between the two test substrates may reflect the importance of sorption in controlling the spatial bioavailability of compounds in soil. (C) 2002 Elsevier Science Ltd. All rights reserved.

AB - The spatial location of microorganisms in the soil three-dimensional structure with respect to their substrates plays an important role in the persistence and turnover of natural and xenobiotic organic compounds. To study the effect of spatial location oil the mineralisation of C-14-2,4-dichlorophenol (2,4-DCP, 0.15 or 0.31 mumol g(-1)) and C-14-glucose (2.77 mumol g(-1)), columns packed with autoclaved soil aggregates (2-5 mm) were used. Using a chloride tracer of water movement, the existence of 'immobile' water, which was by-passed by preferentially flowing 'mobile' water, was demonstrated. By manipulation of the soil Moisture Content, the substrates were putatively placed to these conceptual hydrological domains (immobile and mobile water). Leaching studies revealed that approximately 1.7 (glucose) and 3.4 (2,4-DCP) times the amount of substrate placed in mobile water was recovered in the first 4 fractions of leachate when compared to substrate placed in immobile water. The marked difference in the breakthrough curves was taken as evidence of Successful substrate placement. The 2,4-DCP degrading bacterium, Burkholderia sp. RASCc2, was inoculated in mobile water (1.8-5.2 x 10(7) cells g(-1) soil) and parameters (asymptote, time at maximum rate, calculated maximum rate) describing the mineralisation kinetics of 2,4-DCP and glucose previously added to immobile or mobile water domains were compared. For glucose, there was no significant effect (P > 0.1) of substrate placement Oil any of the mineralisation parameters. However, substrate placement had a significant effect (P < 0.05) on parameters describing 2.4-DCP mineralisation. In particular, 2,4-DCP added in mobile water was mineralised with a greater maximum rate and with a reduced time at maximum rate when compared to 2,4-DCP added to immobile water. The difference in response between the two test substrates may reflect the importance of sorption in controlling the spatial bioavailability of compounds in soil. (C) 2002 Elsevier Science Ltd. All rights reserved.

KW - bioavailability

KW - sequestration

KW - spatial

KW - inoculation

KW - biodegradation

KW - LEGUMINOSARUM BIOVAR TRIFOLII

KW - HABITABLE PORE-SPACE

KW - ORGANIC-COMPOUNDS

KW - SOLUTE TRANSPORT

KW - SOIL

KW - SORPTION

KW - BIODEGRADATION

KW - BIOAVAILABILITY

KW - SEQUESTRATION

KW - PHENANTHRENE

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DO - 10.1016/S0038-0717(01)00212-7

M3 - Article

VL - 34

SP - 531

EP - 539

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -