Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line

M H Grant, S J Duthie, A G Gray, M D Burke

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    129 Citations (Scopus)

    Abstract

    In cultured human hepatoma cells phenolphthalein glucuronidation was increased 3-fold by 2 mM phenobarbitone (PB) in the culture medium but not by 25 microM benz(a)anthracene (BA), while 1-naphthol glucuronidation was not increased by either PB or BA. Ethoxyresorufin O-deethylation (EROD) was increased 15-fold by BA but not by PB, while the O-dealkylations of pentoxyresorufin (PROD) and benzyloxyresorufin (BROD) were increased by either PB or BA. The BROD activity increased by BA was sensitive to inhibition by alpha-naphthoflavone whereas that induced by PB was not. This suggests induction of different cytochrome P-450 isoenzymes. Control Hep G2 cells had similar glucuronide conjugation and cytochrome reductase activities to freshly isolated human adult hepatocytes, but had lower O-dealkylation and elevated microsomal epoxide hydrolase activities.
    Original languageEnglish
    Pages (from-to)4111-6
    Number of pages6
    JournalBiochemical Pharmacology
    Volume37
    Issue number21
    Publication statusPublished - 1988

    Keywords

    • Carcinoma, Hepatocellular
    • Cytochrome P-450 CYP2B1
    • Epoxide Hydrolases
    • Glucuronosyltransferase
    • Humans
    • Liver
    • Liver Neoplasms
    • Mixed Function Oxygenases
    • Oxazines
    • Oxidation-Reduction
    • Oxidoreductases
    • Tumor Cells, Cultured

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