Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging

Hui Zhou, Han Yang, Lin Tang, Yue Wang, Yang Li, Nan Liu, Xiaodong Zeng, Yu Yan, Junzhu Wu, Shizhen Chen, Long Xiao, Yi Yu, Zixin Deng, Hai Deng, Xuechuan Hong (Corresponding Author), Yuling Xiao (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.

Original languageEnglish
Pages (from-to)9448-9454
Number of pages7
JournalJournal of Materials Chemistry A
Volume7
Issue number31
Early online date11 Jul 2019
DOIs
Publication statusPublished - 21 Aug 2019

Keywords

  • CONTRAST AGENT
  • NANOPARTICLES
  • FLUORESCENCE
  • MANGANESE
  • FLUOROPHORES
  • WAVELENGTHS
  • LACTOFERRIN
  • COMPLEXES
  • CHROMIUM
  • COBALT

Cite this

Zhou, H., Yang, H., Tang, L., Wang, Y., Li, Y., Liu, N., ... Xiao, Y. (2019). Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging. Journal of Materials Chemistry A, 7(31), 9448-9454. https://doi.org/10.1039/c9tc01929d

Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging. / Zhou, Hui; Yang, Han; Tang, Lin; Wang, Yue; Li, Yang; Liu, Nan; Zeng, Xiaodong; Yan, Yu; Wu, Junzhu; Chen, Shizhen; Xiao, Long; Yu, Yi; Deng, Zixin; Deng, Hai; Hong, Xuechuan (Corresponding Author); Xiao, Yuling (Corresponding Author).

In: Journal of Materials Chemistry A, Vol. 7, No. 31, 21.08.2019, p. 9448-9454.

Research output: Contribution to journalArticle

Zhou, H, Yang, H, Tang, L, Wang, Y, Li, Y, Liu, N, Zeng, X, Yan, Y, Wu, J, Chen, S, Xiao, L, Yu, Y, Deng, Z, Deng, H, Hong, X & Xiao, Y 2019, 'Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging', Journal of Materials Chemistry A, vol. 7, no. 31, pp. 9448-9454. https://doi.org/10.1039/c9tc01929d
Zhou, Hui ; Yang, Han ; Tang, Lin ; Wang, Yue ; Li, Yang ; Liu, Nan ; Zeng, Xiaodong ; Yan, Yu ; Wu, Junzhu ; Chen, Shizhen ; Xiao, Long ; Yu, Yi ; Deng, Zixin ; Deng, Hai ; Hong, Xuechuan ; Xiao, Yuling. / Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging. In: Journal of Materials Chemistry A. 2019 ; Vol. 7, No. 31. pp. 9448-9454.
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abstract = "The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.",
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note = "Acknowledgements This work was partially supported by grants from the National Natural Science Foundation of China (81773674, 81573383 and 21763002), the NSFHP (2017CFA024, 2017CFB711, and 2016ACA126), the Health Commission of Hubei Province Scientific Research Project (WJ2019M177 and WJ2019M178), the Tibet Autonomous Region Science and Technology Plan Project Key Project (XZ201901-GB-11), the Shenzhen Science and Technology Research Grant (JCYJ20170303170809222) and the Fundamental Research Funds for the Central Universities.",
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T1 - Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging

AU - Zhou, Hui

AU - Yang, Han

AU - Tang, Lin

AU - Wang, Yue

AU - Li, Yang

AU - Liu, Nan

AU - Zeng, Xiaodong

AU - Yan, Yu

AU - Wu, Junzhu

AU - Chen, Shizhen

AU - Xiao, Long

AU - Yu, Yi

AU - Deng, Zixin

AU - Deng, Hai

AU - Hong, Xuechuan

AU - Xiao, Yuling

N1 - Acknowledgements This work was partially supported by grants from the National Natural Science Foundation of China (81773674, 81573383 and 21763002), the NSFHP (2017CFA024, 2017CFB711, and 2016ACA126), the Health Commission of Hubei Province Scientific Research Project (WJ2019M177 and WJ2019M178), the Tibet Autonomous Region Science and Technology Plan Project Key Project (XZ201901-GB-11), the Shenzhen Science and Technology Research Grant (JCYJ20170303170809222) and the Fundamental Research Funds for the Central Universities.

PY - 2019/8/21

Y1 - 2019/8/21

N2 - The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.

AB - The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.

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KW - NANOPARTICLES

KW - FLUORESCENCE

KW - MANGANESE

KW - FLUOROPHORES

KW - WAVELENGTHS

KW - LACTOFERRIN

KW - COMPLEXES

KW - CHROMIUM

KW - COBALT

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U2 - 10.1039/c9tc01929d

DO - 10.1039/c9tc01929d

M3 - Article

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JO - Journal of Materials Chemistry A

JF - Journal of Materials Chemistry A

SN - 2050-7488

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