Modelling of fish interleukin-1 and its receptor

G Scapigliati, S Costantini, G Colonna, A Facchiano, F Buonocore, P B Bossu, C Cunningham, J W Holland, C J Secombes

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

This paper presents original data regarding: the three-dimensional modelling of rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax) IL-1beta, the modelling of trout IL-1 receptor type I (IL-1R(1)) and the modelling of trout IL-1beta bound to its receptor. The 3D models of trout and sea bass IL-1beta molecules were predicted by comparison with those already available of human and mouse, and, in both cases, a structure consisting of a beta-trefoil fold was obtained, a motif well conserved during evolution of IL-1beta-related proteins. Moreover, a model for the rainbow trout IL-1 receptor alone and complexed with IL-1beta was predicted and compared to the murine model. Both ligand-receptor complex models were compared with the known crystal structure of the human IL-1beta/IL-IR complex. A cross-interaction of trout IL-1beta with the mouse receptor was also simulated. Such analysis and the predicted interaction energies calculated from the models have helped to explain the different biological efficacies of mammalian and fish IL-1beta molecules in assays based on mammalian target cells. The obtained data are discussed on the basis of evolutionary and applied perspectives. (C) 2003 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)429-441
Number of pages13
JournalDevelopmental and Comparative Immunology
Volume28
DOIs
Publication statusPublished - 2004

Keywords

  • cytokines
  • interleukin-1
  • interleukin-1 receptors
  • rainbow trout
  • sea bass
  • IL-1 modelling
  • IL-1 structure
  • IL-IRI
  • comparative modelling
  • TROUT ONCORHYNCHUS-MYKISS
  • GROWTH-FACTOR-BETA
  • CYPRINUS-CARPIO L
  • NF-KAPPA-B
  • RAINBOW-TROUT
  • MOLECULAR-CLONING
  • SIGNAL-TRANSDUCTION
  • EXPRESSION ANALYSIS
  • KIDNEY LEUKOCYTES
  • CRYSTAL-STRUCTURE

Cite this

Scapigliati, G., Costantini, S., Colonna, G., Facchiano, A., Buonocore, F., Bossu, P. B., ... Secombes, C. J. (2004). Modelling of fish interleukin-1 and its receptor. Developmental and Comparative Immunology, 28, 429-441. https://doi.org/10.1016/j.dci.2003.09.014

Modelling of fish interleukin-1 and its receptor. / Scapigliati, G ; Costantini, S ; Colonna, G ; Facchiano, A ; Buonocore, F ; Bossu, P B ; Cunningham, C ; Holland, J W ; Secombes, C J .

In: Developmental and Comparative Immunology, Vol. 28, 2004, p. 429-441.

Research output: Contribution to journalArticle

Scapigliati, G, Costantini, S, Colonna, G, Facchiano, A, Buonocore, F, Bossu, PB, Cunningham, C, Holland, JW & Secombes, CJ 2004, 'Modelling of fish interleukin-1 and its receptor', Developmental and Comparative Immunology, vol. 28, pp. 429-441. https://doi.org/10.1016/j.dci.2003.09.014
Scapigliati G, Costantini S, Colonna G, Facchiano A, Buonocore F, Bossu PB et al. Modelling of fish interleukin-1 and its receptor. Developmental and Comparative Immunology. 2004;28:429-441. https://doi.org/10.1016/j.dci.2003.09.014
Scapigliati, G ; Costantini, S ; Colonna, G ; Facchiano, A ; Buonocore, F ; Bossu, P B ; Cunningham, C ; Holland, J W ; Secombes, C J . / Modelling of fish interleukin-1 and its receptor. In: Developmental and Comparative Immunology. 2004 ; Vol. 28. pp. 429-441.
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abstract = "This paper presents original data regarding: the three-dimensional modelling of rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax) IL-1beta, the modelling of trout IL-1 receptor type I (IL-1R(1)) and the modelling of trout IL-1beta bound to its receptor. The 3D models of trout and sea bass IL-1beta molecules were predicted by comparison with those already available of human and mouse, and, in both cases, a structure consisting of a beta-trefoil fold was obtained, a motif well conserved during evolution of IL-1beta-related proteins. Moreover, a model for the rainbow trout IL-1 receptor alone and complexed with IL-1beta was predicted and compared to the murine model. Both ligand-receptor complex models were compared with the known crystal structure of the human IL-1beta/IL-IR complex. A cross-interaction of trout IL-1beta with the mouse receptor was also simulated. Such analysis and the predicted interaction energies calculated from the models have helped to explain the different biological efficacies of mammalian and fish IL-1beta molecules in assays based on mammalian target cells. The obtained data are discussed on the basis of evolutionary and applied perspectives. (C) 2003 Elsevier Ltd. All rights reserved.",
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AU - Costantini, S

AU - Colonna, G

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AU - Bossu, P B

AU - Cunningham, C

AU - Holland, J W

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N2 - This paper presents original data regarding: the three-dimensional modelling of rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax) IL-1beta, the modelling of trout IL-1 receptor type I (IL-1R(1)) and the modelling of trout IL-1beta bound to its receptor. The 3D models of trout and sea bass IL-1beta molecules were predicted by comparison with those already available of human and mouse, and, in both cases, a structure consisting of a beta-trefoil fold was obtained, a motif well conserved during evolution of IL-1beta-related proteins. Moreover, a model for the rainbow trout IL-1 receptor alone and complexed with IL-1beta was predicted and compared to the murine model. Both ligand-receptor complex models were compared with the known crystal structure of the human IL-1beta/IL-IR complex. A cross-interaction of trout IL-1beta with the mouse receptor was also simulated. Such analysis and the predicted interaction energies calculated from the models have helped to explain the different biological efficacies of mammalian and fish IL-1beta molecules in assays based on mammalian target cells. The obtained data are discussed on the basis of evolutionary and applied perspectives. (C) 2003 Elsevier Ltd. All rights reserved.

AB - This paper presents original data regarding: the three-dimensional modelling of rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax) IL-1beta, the modelling of trout IL-1 receptor type I (IL-1R(1)) and the modelling of trout IL-1beta bound to its receptor. The 3D models of trout and sea bass IL-1beta molecules were predicted by comparison with those already available of human and mouse, and, in both cases, a structure consisting of a beta-trefoil fold was obtained, a motif well conserved during evolution of IL-1beta-related proteins. Moreover, a model for the rainbow trout IL-1 receptor alone and complexed with IL-1beta was predicted and compared to the murine model. Both ligand-receptor complex models were compared with the known crystal structure of the human IL-1beta/IL-IR complex. A cross-interaction of trout IL-1beta with the mouse receptor was also simulated. Such analysis and the predicted interaction energies calculated from the models have helped to explain the different biological efficacies of mammalian and fish IL-1beta molecules in assays based on mammalian target cells. The obtained data are discussed on the basis of evolutionary and applied perspectives. (C) 2003 Elsevier Ltd. All rights reserved.

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KW - sea bass

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KW - comparative modelling

KW - TROUT ONCORHYNCHUS-MYKISS

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KW - EXPRESSION ANALYSIS

KW - KIDNEY LEUKOCYTES

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