Moderate maternal vitamin A deficiency alters myogenic regulatory protein expression and perinatal organ growth in the rat

D Downie, C Antipatis, Margaret Inkster Delday, Charlotte Maltin, Alan Arthur Sneddon

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Abstract

Vitamin A deficiency is one of the most common dietary deficiencies in the developing world and is a major health concern where it is associated with increased risk of fetal and infant mortality and morbidity. Early studies in the rat demonstrated that, in addition to respiratory problems, neonates showed evidence of mobility problems in response to moderate vitamin A deficiency. This study investigated whether moderate deficiency of this vitamin plays a role in regulating key skeletal muscle regulatory pathways during development. Thirty female rats were fed vitamin A-moderate (VAM) or vitamin A-sufficient diets from weaning and throughout pregnancy. Fetal and neonatal hindlimb and muscle samples were collected on days 13.5, 15.5, 17.5, and 19.5 of pregnancy and 1 day following birth. Mothers fed the VAM diet had reduced retinol concentrations at all time points studied ( P < 0.01), and neonates had reduced relative lung weights ( P < 0.01). Fetal weight and survival did not differ between groups but neonatal survival was lower in the VAM group where neonates had increased relative heart weights ( P < 0.05). Analysis of myogenic regulatory factor expression and calcineurin signaling in fetuses and neonates demonstrated decreased protein levels of myf5 [50% at 17.5 dg ( P < 0.05)], myogenin [70% at birth (P < 0.001)], and myosin heavy chain fast [ 50% at birth ( P < 0.05)] in response to moderate vitamin A deficiency. Overall, these changes suggest that vitamin A status during pregnancy may have important implications for fetal muscle development and subsequent muscle function in the offspring.

Original languageEnglish
Pages (from-to)R73-R79
Number of pages7
JournalAmerican Journal of Physiology-Regulatory Integrative and Comparative Physiology
Volume288
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • retinoic acid
  • calcineurin
  • skeletal muscle
  • neonate
  • skeletal muscle differentiation
  • gene expression
  • guinea pig
  • fiber type
  • NF-AT
  • pathway
  • slow
  • myod
  • undernutrition

Cite this

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title = "Moderate maternal vitamin A deficiency alters myogenic regulatory protein expression and perinatal organ growth in the rat",
abstract = "Vitamin A deficiency is one of the most common dietary deficiencies in the developing world and is a major health concern where it is associated with increased risk of fetal and infant mortality and morbidity. Early studies in the rat demonstrated that, in addition to respiratory problems, neonates showed evidence of mobility problems in response to moderate vitamin A deficiency. This study investigated whether moderate deficiency of this vitamin plays a role in regulating key skeletal muscle regulatory pathways during development. Thirty female rats were fed vitamin A-moderate (VAM) or vitamin A-sufficient diets from weaning and throughout pregnancy. Fetal and neonatal hindlimb and muscle samples were collected on days 13.5, 15.5, 17.5, and 19.5 of pregnancy and 1 day following birth. Mothers fed the VAM diet had reduced retinol concentrations at all time points studied ( P < 0.01), and neonates had reduced relative lung weights ( P < 0.01). Fetal weight and survival did not differ between groups but neonatal survival was lower in the VAM group where neonates had increased relative heart weights ( P < 0.05). Analysis of myogenic regulatory factor expression and calcineurin signaling in fetuses and neonates demonstrated decreased protein levels of myf5 [50{\%} at 17.5 dg ( P < 0.05)], myogenin [70{\%} at birth (P < 0.001)], and myosin heavy chain fast [ 50{\%} at birth ( P < 0.05)] in response to moderate vitamin A deficiency. Overall, these changes suggest that vitamin A status during pregnancy may have important implications for fetal muscle development and subsequent muscle function in the offspring.",
keywords = "retinoic acid, calcineurin, skeletal muscle, neonate, skeletal muscle differentiation, gene expression, guinea pig, fiber type, NF-AT, pathway, slow, myod, undernutrition",
author = "D Downie and C Antipatis and Delday, {Margaret Inkster} and Charlotte Maltin and Sneddon, {Alan Arthur}",
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T1 - Moderate maternal vitamin A deficiency alters myogenic regulatory protein expression and perinatal organ growth in the rat

AU - Downie, D

AU - Antipatis, C

AU - Delday, Margaret Inkster

AU - Maltin, Charlotte

AU - Sneddon, Alan Arthur

PY - 2005/1

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N2 - Vitamin A deficiency is one of the most common dietary deficiencies in the developing world and is a major health concern where it is associated with increased risk of fetal and infant mortality and morbidity. Early studies in the rat demonstrated that, in addition to respiratory problems, neonates showed evidence of mobility problems in response to moderate vitamin A deficiency. This study investigated whether moderate deficiency of this vitamin plays a role in regulating key skeletal muscle regulatory pathways during development. Thirty female rats were fed vitamin A-moderate (VAM) or vitamin A-sufficient diets from weaning and throughout pregnancy. Fetal and neonatal hindlimb and muscle samples were collected on days 13.5, 15.5, 17.5, and 19.5 of pregnancy and 1 day following birth. Mothers fed the VAM diet had reduced retinol concentrations at all time points studied ( P < 0.01), and neonates had reduced relative lung weights ( P < 0.01). Fetal weight and survival did not differ between groups but neonatal survival was lower in the VAM group where neonates had increased relative heart weights ( P < 0.05). Analysis of myogenic regulatory factor expression and calcineurin signaling in fetuses and neonates demonstrated decreased protein levels of myf5 [50% at 17.5 dg ( P < 0.05)], myogenin [70% at birth (P < 0.001)], and myosin heavy chain fast [ 50% at birth ( P < 0.05)] in response to moderate vitamin A deficiency. Overall, these changes suggest that vitamin A status during pregnancy may have important implications for fetal muscle development and subsequent muscle function in the offspring.

AB - Vitamin A deficiency is one of the most common dietary deficiencies in the developing world and is a major health concern where it is associated with increased risk of fetal and infant mortality and morbidity. Early studies in the rat demonstrated that, in addition to respiratory problems, neonates showed evidence of mobility problems in response to moderate vitamin A deficiency. This study investigated whether moderate deficiency of this vitamin plays a role in regulating key skeletal muscle regulatory pathways during development. Thirty female rats were fed vitamin A-moderate (VAM) or vitamin A-sufficient diets from weaning and throughout pregnancy. Fetal and neonatal hindlimb and muscle samples were collected on days 13.5, 15.5, 17.5, and 19.5 of pregnancy and 1 day following birth. Mothers fed the VAM diet had reduced retinol concentrations at all time points studied ( P < 0.01), and neonates had reduced relative lung weights ( P < 0.01). Fetal weight and survival did not differ between groups but neonatal survival was lower in the VAM group where neonates had increased relative heart weights ( P < 0.05). Analysis of myogenic regulatory factor expression and calcineurin signaling in fetuses and neonates demonstrated decreased protein levels of myf5 [50% at 17.5 dg ( P < 0.05)], myogenin [70% at birth (P < 0.001)], and myosin heavy chain fast [ 50% at birth ( P < 0.05)] in response to moderate vitamin A deficiency. Overall, these changes suggest that vitamin A status during pregnancy may have important implications for fetal muscle development and subsequent muscle function in the offspring.

KW - retinoic acid

KW - calcineurin

KW - skeletal muscle

KW - neonate

KW - skeletal muscle differentiation

KW - gene expression

KW - guinea pig

KW - fiber type

KW - NF-AT

KW - pathway

KW - slow

KW - myod

KW - undernutrition

U2 - 10.1152/ajpregu.00186.2004

DO - 10.1152/ajpregu.00186.2004

M3 - Article

VL - 288

SP - R73-R79

JO - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 1

ER -