Modular gene over-expression strategies for Candida albicans

Vitor Cabral, Murielle Chauvel, Arnaud Firon, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Anne Munro, Christophe d'Enfert

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.
Original languageEnglish
Title of host publicationHost-Fungus Interactions
Subtitle of host publicationMethods and Protocols
EditorsAlexandra Brand, Donna MacCallum
Place of PublicationNewYork
PublisherHumana Press
Pages227-244
Number of pages18
Volume2
ISBN (Print)9781617795381
DOIs
Publication statusPublished - 29 Feb 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume845
ISSN (Print)1064-3745

Keywords

  • Gateway cloning
  • Genetic transformation
  • Integrative vectors
  • Over-expression
  • Strain storage
  • TAP tagging
  • Western blotting

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