Modular gene over-expression strategies for Candida albicans

Vitor Cabral, Murielle Chauvel, Arnaud Firon, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Anne Munro, Christophe d'Enfert

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.
Original languageEnglish
Title of host publicationHost-Fungus Interactions
Subtitle of host publicationMethods and Protocols
EditorsAlexandra Brand, Donna MacCallum
Place of PublicationNewYork
PublisherHumana Press
Pages227-244
Number of pages18
Volume2
ISBN (Print)9781617795381
DOIs
Publication statusPublished - 29 Feb 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume845
ISSN (Print)1064-3745

Fingerprint

Candida albicans
Gene Expression
Genomics
Genes
Genome
Phenotype

Keywords

  • Gateway cloning
  • Genetic transformation
  • Integrative vectors
  • Over-expression
  • Strain storage
  • TAP tagging
  • Western blotting

Cite this

Cabral, V., Chauvel, M., Firon, A., Nesseir, A., Bachellier-Bassi, S., Chaudhari, Y., ... d'Enfert, C. (2012). Modular gene over-expression strategies for Candida albicans. In A. Brand, & D. MacCallum (Eds.), Host-Fungus Interactions: Methods and Protocols (Vol. 2, pp. 227-244). (Methods in Molecular Biology; Vol. 845). NewYork: Humana Press. https://doi.org/10.1007/978-1-61779-539-8_15

Modular gene over-expression strategies for Candida albicans. / Cabral, Vitor ; Chauvel, Murielle ; Firon, Arnaud; Nesseir, Audrey; Bachellier-Bassi, Sophie; Chaudhari, Yogesh ; Munro, Carol Anne; d'Enfert, Christophe.

Host-Fungus Interactions: Methods and Protocols. ed. / Alexandra Brand; Donna MacCallum. Vol. 2 NewYork : Humana Press, 2012. p. 227-244 (Methods in Molecular Biology; Vol. 845).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cabral, V, Chauvel, M, Firon, A, Nesseir, A, Bachellier-Bassi, S, Chaudhari, Y, Munro, CA & d'Enfert, C 2012, Modular gene over-expression strategies for Candida albicans. in A Brand & D MacCallum (eds), Host-Fungus Interactions: Methods and Protocols. vol. 2, Methods in Molecular Biology, vol. 845, Humana Press, NewYork, pp. 227-244. https://doi.org/10.1007/978-1-61779-539-8_15
Cabral V, Chauvel M, Firon A, Nesseir A, Bachellier-Bassi S, Chaudhari Y et al. Modular gene over-expression strategies for Candida albicans. In Brand A, MacCallum D, editors, Host-Fungus Interactions: Methods and Protocols. Vol. 2. NewYork: Humana Press. 2012. p. 227-244. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-539-8_15
Cabral, Vitor ; Chauvel, Murielle ; Firon, Arnaud ; Nesseir, Audrey ; Bachellier-Bassi, Sophie ; Chaudhari, Yogesh ; Munro, Carol Anne ; d'Enfert, Christophe. / Modular gene over-expression strategies for Candida albicans. Host-Fungus Interactions: Methods and Protocols. editor / Alexandra Brand ; Donna MacCallum. Vol. 2 NewYork : Humana Press, 2012. pp. 227-244 (Methods in Molecular Biology).
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